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Team:Cornell/Notebook/Wetlab
From 2012.igem.org
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=June= | =June= | ||
| - | ==June 28th, Wednesday== | + | ==June 10th-16th== |
| + | ===June 13th, Wednesday=== | ||
| + | *Set up [[Team:Cornell/Notebook/Phusion_PCR|PCR]] for four fragments of the nah operon as well as the pBMT-1 backbone, using primers designed to mutate cut-sites concurrently with Gibson Assembly. Also set up a PCR for the entire nah operon. Due to length of the fragments, a longer extension time was chosen. | ||
| + | *If Gibson Assembly fails, but we are able to PCR the entire 10kb nah operon, an alternate method of mutation using three sequential site-directed mutageneses will be pursued. | ||
| + | **''Work done by: Caleb, Claire, Dylan, Spencer, Steven, and Swati'' | ||
| + | |||
| + | ===June 14th, Thursday=== | ||
| + | *PCR only amplified nah1, nah3, and nah4 - longer fragments (nah2, the full nah operon, and pBMT-1) were not identified on the PCR product gel. Set up another [[Team:Cornell/Notebook/Phusion_PCR|PCR]] with shorter extension time. | ||
| + | |||
| + | ===June 15th, Friday=== | ||
| + | *PCR of four out of five products visible on gel. Set up PCR of pBMT-1, final fragment required for Gibson Assembly of the nah operon. | ||
| + | |||
| + | ==June 17th-23rd== | ||
| + | |||
| + | ==June 24th-30th== | ||
| + | |||
| + | |||
| + | ===June 28th, Wednesday=== | ||
[[Image:2012_6_28_StevenArchieGelLoading.jpg|thumb|right|Stephen and Archie Gel purifying our arsR construct]] | [[Image:2012_6_28_StevenArchieGelLoading.jpg|thumb|right|Stephen and Archie Gel purifying our arsR construct]] | ||
*Gel purified arsR construct | *Gel purified arsR construct | ||
Revision as of 02:30, 18 July 2012
| Home | Team | Project | Parts | Modeling | Notebook | Protocols | Safety | Attributions |
|---|
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June
June 10th-16th
June 13th, Wednesday
- Set up PCR for four fragments of the nah operon as well as the pBMT-1 backbone, using primers designed to mutate cut-sites concurrently with Gibson Assembly. Also set up a PCR for the entire nah operon. Due to length of the fragments, a longer extension time was chosen.
- If Gibson Assembly fails, but we are able to PCR the entire 10kb nah operon, an alternate method of mutation using three sequential site-directed mutageneses will be pursued.
- Work done by: Caleb, Claire, Dylan, Spencer, Steven, and Swati
June 14th, Thursday
- PCR only amplified nah1, nah3, and nah4 - longer fragments (nah2, the full nah operon, and pBMT-1) were not identified on the PCR product gel. Set up another PCR with shorter extension time.
June 15th, Friday
- PCR of four out of five products visible on gel. Set up PCR of pBMT-1, final fragment required for Gibson Assembly of the nah operon.
June 17th-23rd
June 24th-30th
June 28th, Wednesday
- Gel purified arsR construct
July
=August=
