Team:WashU/Week8

From 2012.igem.org

(Difference between revisions)
Line 154: Line 154:
<td>UGTCs2: digest with X and P</td>
<td>UGTCs2: digest with X and P</td>
</tr>
</tr>
 +
 +
<tr>
 +
<td>2: digest with P</td>
 +
</tr>
 +
 +
<tr>
 +
<td>2: digest with B</td>
 +
</tr>
 +
</tbody>
</tbody>

Revision as of 21:45, 16 July 2012



Monday, July 16

We ran multiple digests today, following the standard biobrick assembly protocol.

Digestions
Z and C: digest with P and E
Ligation 2: digest with X and P
Ligation 2: digest with X
Z and C: digest with E
ZCD: digest with X and P
UGTCs2: digest with X and P
2: digest with P
2: digest with B

The gel of these digests can be shown below. [INSERT GEL PICTURE HERE]

In addition, we ran several PCRs today. Following the NEB protocol, we ran four PCRs to amplify the CCD plasmid (PUT PROPER NAME HERE) and also tried four colony PCRs, using colonies 2, 4, 13 and ______.

To enable amplification of our plasmid PSL2131, PsbA2, and CS42S, we designed primers to multiply the aforementioned DNA pieces.
Tuesday, July 17


Wednesday, July 18


Thursday, July 19


Friday, July 20


Back to Weekly Log

Retrieved from "http://2012.igem.org/Team:WashU/Week8"