Diego.marcos (Talk | contribs) (DNA concestration) |
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{{:Team:EPF-Lausanne/Template/LabPresence|Sowmya, Diego, Sander}} | {{:Team:EPF-Lausanne/Template/LabPresence|Sowmya, Diego, Sander}} | ||
| - | Removal of remaining salts: Added 500ul of TE to the dried DNA pellet. Put in incubator for 1h30 to dissolve the pellet. Centrifuged at 10'000 rpm for 10min (in eppendorfs). Put the supernatent into another eppendorf and put in storage. | + | Removal of remaining salts: Added 500ul of TE to the dried DNA pellet. Put in incubator for 1h30 to dissolve the pellet. Centrifuged at 10'000 rpm for 10min (in eppendorfs). Put the supernatent into another eppendorf and put in storage, in a yellow box in the cell lab fridge. |
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| + | Quantifying the amount of DNA: | ||
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| + | * RO plasmid: 407.2 ng/µl | ||
| + | * LovTAP plasmid: 294.5 ng/µl | ||
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| + | For both the 260/280 ratio was 1.865 and 260/230 was 2.315, | ||
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{{:Team:EPF-Lausanne/Template/Footer}} | {{:Team:EPF-Lausanne/Template/Footer}} | ||
Removal of remaining salts: Added 500ul of TE to the dried DNA pellet. Put in incubator for 1h30 to dissolve the pellet. Centrifuged at 10'000 rpm for 10min (in eppendorfs). Put the supernatent into another eppendorf and put in storage, in a yellow box in the cell lab fridge.
Quantifying the amount of DNA:
For both the 260/280 ratio was 1.865 and 260/230 was 2.315,
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