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Team:Nevada/Protocols
From 2012.igem.org
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[[Team:Nevada/Notebook|Back to Notebook]] . [[Team:Nevada/Recipes|View Recipes]]<br/><br/> | [[Team:Nevada/Notebook|Back to Notebook]] . [[Team:Nevada/Recipes|View Recipes]]<br/><br/> | ||
===Phusion PCR=== | ===Phusion PCR=== | ||
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===Qiagen Miniprep kit=== | ===Qiagen Miniprep kit=== | ||
www.qiagen.com/hb/qiaprepminiprep<br/> | www.qiagen.com/hb/qiaprepminiprep<br/> | ||
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Revision as of 18:31, 12 July 2012
Phusion PCR
Thermocycling conditions:
Initial Denaturation: 98°C for 30 seconds
25-35 cycles:
- 98°C for 10 seconds
- 55°C, 60°C, 65°C for 15 seconds
- 72°C for 15 seconds
Final Extension: 72°C for 5 minutes
Qiagen Miniprep kit
www.qiagen.com/hb/qiaprepminiprep
