Team:MIT/ResultsOverview

From 2012.igem.org

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The MIT iGEM team sought to unify aspects from the fields of DNA computing and synthetic biology in one project. We built upon an existing in vitro method of nucleic acid computation, designing new components and extending strand displacement to in vivo applications.<br><br> Over the past few months we have:
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The 2012 MIT iGEM team sought to unify aspects from the fields of DNA computing and synthetic biology in one project. We built upon an existing in vitro method of nucleic acid computation, designing new components and extending strand displacement to in vivo applications.
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<br>
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<br> Over the past few months we have:
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<li> established the viability of RNA as an alternative processing medium to DNA
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<li> established the <a href = "https://2012.igem.org/Team:MIT/TheKeyReaction">viability of RNA</a> as an alternative processing medium to DNA
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<li> demonstrated RNA strand displacement <i>in vivo</i> and <i>in vitro</i>
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<li> demonstrated RNA strand displacement <a href = "https://2012.igem.org/Team:MIT/TheKeyReaction#exciting"><i>in vivo</i></a> and <a href = "https://2012.igem.org/Team:MIT/TheKeyReaction#SDbio"><i>in vitro</i></a>
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<li> successfully delivered DNA and RNA into mammalian cells
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<li><a href = "https://2012.igem.org/Team:MIT/TheKeyReaction#NADbio">delivered DNA and RNA</a> into mammalian cells successfully
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<li> designed, modeled, and tested a DNA-based NOT gate, compatible with strand displacement circuits
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<li> designed, modeled, and tested a <a href = "https://2012.igem.org/Team:MIT/NOTGate#m2bio">DNA-based NOT gate</a>, compatible with strand displacement circuits
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<li> designed, modeled, and tested a modular mRNA sensor along with an inverting sensor, both compatible with strand displacement circuits
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<li> designed, modeled, and tested a <a href = "https://2012.igem.org/Team:MIT/Sensing">modular mRNA sensor</a> along with an inverting sensor, both compatible with strand displacement circuits
<li> designed, modeled, and tested various forms of regulating gene expression at the RNA level, compatible with strand displacement circuits, using:
<li> designed, modeled, and tested various forms of regulating gene expression at the RNA level, compatible with strand displacement circuits, using:
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<li> miRNA-induced RNAi knockdown
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<li> <a href = "https://2012.igem.org/Team:MIT/Actuation#m6bio">miRNA-induced RNAi knockdown</a>
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<li> Decoy & TuD mediated double-repression systems
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<li> <a href = "https://2012.igem.org/Team:MIT/Actuation#m40bio">Decoy & TuD</a> mediated double-repression systems
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<li> self-cleaving Hammerhead ribozymes
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<li> self-cleaving <a href = "https://2012.igem.org/Team:MIT/CircuitProduction#HHs_productionbio">Hammerhead ribozymes</a>
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<li> characterized various new MammoBlock parts for transcribing short pieces of RNA in mammalian cells.
<li> characterized various new MammoBlock parts for transcribing short pieces of RNA in mammalian cells.
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<li> physically submitted 22 of our best <a href="https://2012.igem.org/Team:MIT/ResultsBiobricks">standard parts</a> to the Registry of Biological Parts
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<td><a href="https://2012.igem.org/Team:MIT/NOTGate#NOTgate_modelbio">
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<img src = "https://static.igem.org/mediawiki/2012/e/e5/MIT-2012-computationalmodelling.png" width = "300"></img>
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<img src = "https://static.igem.org/mediawiki/2012/e/e5/MIT-2012-computationalmodelling.png" width = "250"></img></a>
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In addition to our experimental results, we programmed computational models to inform our design decisions. We used Visual DSD to design a strand displacement <a href = "https://2012.igem.org/Team:MIT/ResultsProcessing#model">NOT gate</a>, and we used Visual GEC to design the <a href = "https://2012.igem.org/Team:MIT/ResultsSensing#sensing2bio">mRNA sensor</a> and an <a href = "https://2012.igem.org/Team:MIT/ResultsSensing#sensing2bio">inverted mRNA sensor</a>. Check it out!
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In addition to our experimental results, we programmed computational models to inform our design decisions. We used Visual DSD to design a strand displacement <a href = "https://2012.igem.org/Team:MIT/NOTGate#NOTgate_modelbio">NOT gate</a>, and we used Visual GEC to design the <a href = "https://2012.igem.org/Team:MIT/Sensing#sensing2bio">mRNA sensor</a> and an <a href = "https://2012.igem.org/Team:MIT/Sensing#sensing2bio">inverted mRNA sensor</a>.
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Latest revision as of 02:34, 28 September 2013

iGEM 2012

Overview

  • Results Overview

Circuit Production

  • Short RNA Production
  • Circuit Production: Hammerhead Ribozymes

NOT Gate

  • Design
  • Modeling
  • In Vitro Results

Sensing

  • Design
  • Modeling
  • In Vitro Results

Actuation

  • TuD and Decoy RNAs
  • Modulating Hammerheads

The Key Reaction

  • Design
  • Nucleic Acid Delivery
  • Experimental Strand Displacement

Our BioBricks

  • Favorites
  • All BioBricks

Attributions

  • Attributions
The 2012 MIT iGEM team sought to unify aspects from the fields of DNA computing and synthetic biology in one project. We built upon an existing in vitro method of nucleic acid computation, designing new components and extending strand displacement to in vivo applications.

Over the past few months we have:
  • established the viability of RNA as an alternative processing medium to DNA
  • demonstrated RNA strand displacement in vivo and in vitro
  • delivered DNA and RNA into mammalian cells successfully
  • designed, modeled, and tested a DNA-based NOT gate, compatible with strand displacement circuits
  • designed, modeled, and tested a modular mRNA sensor along with an inverting sensor, both compatible with strand displacement circuits
  • designed, modeled, and tested various forms of regulating gene expression at the RNA level, compatible with strand displacement circuits, using:
  • characterized various new MammoBlock parts for transcribing short pieces of RNA in mammalian cells.
  • physically submitted 22 of our best standard parts to the Registry of Biological Parts
In addition to our experimental results, we programmed computational models to inform our design decisions. We used Visual DSD to design a strand displacement NOT gate, and we used Visual GEC to design the mRNA sensor and an inverted mRNA sensor.

Retrieved from "http://2012.igem.org/Team:MIT/ResultsOverview"