Team:Marburg SYNMIKRO

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== '''<html>&#160;</html>Recombinator:''' <br/> <html><sup>&#160;</sup></html>An intelligent Genetically Engineered Slot Machine (iGESM) ==
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The vertebrate immune system produces billions of different antibodies. This diversity is generated by random VDJ-recombination of a limited number of antibody subfragments. This inspired us to construct an automatic recombination system in <i>E. coli</i> that generates large numbers of novel proteins by combinatorial fusion of functional domains. We used the site-specific DNA recombinase Gin of bacteriophage Mu that depends on the presence of a DNA enhancer element for efficient recombination. This allowed us to construct a system, called “The Recombinator”, which automatically shuts down after successful recombination. We visualized the randomizing function of our genetically engineered slot machine by combining fluorescent colors with cellular localization domains. By scaling up the number of recombination modules and functional domains our system will be able to generate a multitude of new proteins. We envision that “The Recombinator” will serve as a tool to create novel enzymatic activities for innovative drug design, environmental detoxification and metabolic engineering.
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<h3 style="z-index:1000;" >Team mixer</h3><a style="float:right; position: relative; top:-25px; z-index:1000;" href="https://2012.igem.org/Team:Marburg_SYNMIKRO/TeamProfile">[Team members]</a>
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<a href="https://twitter.com/iGESM_Marburg" class="twitter-follow-button" data-show-count="false" data-lang="de">@iGESM_Marburg folgen</a>
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{|align="justify"
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:Marburg_SYNMIKRO_logo.png|200px|right|frame]]
 
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|-
 
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|
 
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:Marburg_SYNMIKRO_team.png|right|frame|Your team picture]]
 
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|-
 
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|
 
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|align="center"|[[Team:Marburg_SYNMIKRO | Team Marburg_SYNMIKRO]]
 
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|}
 
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<!--- The Mission, Experiments --->
 
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!align="center"|[[Team:Marburg_SYNMIKRO|Home]]
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!align="center"|[[Team:Marburg_SYNMIKRO/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Marburg_SYNMIKRO Official Team Profile]
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float:left;
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!align="center"|[[Team:Marburg_SYNMIKRO/Project|Project]]
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position:relative;
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!align="center"|[[Team:Marburg_SYNMIKRO/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Marburg_SYNMIKRO/Modeling|Modeling]]
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}
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!align="center"|[[Team:Marburg_SYNMIKRO/Notebook|Notebook]]
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!align="center"|[[Team:Marburg_SYNMIKRO/Safety|Safety]]
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!align="center"|[[Team:Marburg_SYNMIKRO/Attributions|Attributions]]
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"<b>Daniel Kleinsorge</b><br><br>text",
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"<b>Oliver Schauer</b><br><br>text",
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"<b>Prof. Michael Bölker</b><br><br>text",
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"<b>Marian Vogt</b><br><br>text",
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"<b>Agnieszka Zarzycka</b><br><br>text",
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"<b>Oliver Leicht</b><br><br>text",
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"<b>Margarita Derbenev</b><br><br>text",
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"<b>Elisabeth Ignatz</b><br><br>text",
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"<b>Julian Pechstein</b><br><br>text",
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"<b>Johannes Schuh</b><br><br>text",
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"<b>Max Diesner</b><br><br>text",
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"<b>Svetlana Uscherenko</b><br><br>text"
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);
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<br>
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<a onclick="random(); " class="btn"><span class="blink">Recombine!</span></a>
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== Bacterial combinatorics ==
 +
 
 +
As proof-of-principle of our recombinator system we created combinatorial fusions of bacterial intracellular localization domains with different fluorescent proteins. We expected to generate a mixture of bacteria that glow in different colors and at different positions in all possible combinations.
 +
<br/>
 +
<br/>
 +
[[File:Syn_MR_bunte_Maus.png|center|420px]]
 +
 
 +
Here, we have dealt only with few modules, but with additional recombination modules, the number of possible combinations raises exponentially.
 +
<html>
 +
</div>
 +
<div class="conboxb">
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</html>
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[[File:Syn_MR_Logo.png|link=http://www.uni-marburg.de|left|400px]][[File:Syn_MR_synmikro_farbe.gif|link=http://www.synmikro.com/en/|right|400px]]
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<html>
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Latest revision as of 13:25, 23 September 2013

Main Page

 Recombinator:
 An intelligent Genetically Engineered Slot Machine (iGESM)

The vertebrate immune system produces billions of different antibodies. This diversity is generated by random VDJ-recombination of a limited number of antibody subfragments. This inspired us to construct an automatic recombination system in E. coli that generates large numbers of novel proteins by combinatorial fusion of functional domains. We used the site-specific DNA recombinase Gin of bacteriophage Mu that depends on the presence of a DNA enhancer element for efficient recombination. This allowed us to construct a system, called “The Recombinator”, which automatically shuts down after successful recombination. We visualized the randomizing function of our genetically engineered slot machine by combining fluorescent colors with cellular localization domains. By scaling up the number of recombination modules and functional domains our system will be able to generate a multitude of new proteins. We envision that “The Recombinator” will serve as a tool to create novel enzymatic activities for innovative drug design, environmental detoxification and metabolic engineering.

Team mixer

[Team members]

Bacterial combinatorics

As proof-of-principle of our recombinator system we created combinatorial fusions of bacterial intracellular localization domains with different fluorescent proteins. We expected to generate a mixture of bacteria that glow in different colors and at different positions in all possible combinations.

Syn MR bunte Maus.png

Here, we have dealt only with few modules, but with additional recombination modules, the number of possible combinations raises exponentially.

Syn MR Logo.png
400px

Retrieved from "http://2012.igem.org/Team:Marburg_SYNMIKRO"