Team:WHU-China

From 2012.igem.org

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(47 intermediate revisions not shown)
Line 15: Line 15:
Home : [
Home : [
{
{
-
title : "<strong>What's new</strong>",
+
title : "<strong>What's New</strong>",
-
picArray : [new picObj("http://partsregistry.org/wiki/images/f/ff/%E5%85%A8%E6%99%AF%E5%8A%A0%E6%AD%BB%E4%BA%A11.png", "Panorama of the Whole Project Scheme", "Four novel devices, Fatty Acid Degradation, Cellulose Synthesis, Colonization and Death to ensure high efficiency orchestrate calorie absorption and bacteria survival and make you slim!", "https://2012.igem.org/Team:WHU-China/Project?catalog=0"),
+
picArray : [
-
new picObj("https://static.igem.org/mediawiki/2012/d/d9/A_picture_of_Pcar.png", "Pcar: Promoter of CRP As a Repressor", "By inserting CRP-binding site to overlap with the -10 region of a constitutive promoter, Pcar is able to be induced by glucose, while CRP serves as a repressor rather than an activator.", "https://2012.igem.org/Team:WHU-China/Project?catalog=2#Pcar"),
+
new picObj("https://static.igem.org/mediawiki/2012/e/e3/What%27s_new_first.png","Overall Project", "Compared with traditional methods of losing weight, our probiotic-- E.coslim has three advantages: monitoring, high efficiency and long term efficiency.", "https://2012.igem.org/Team:WHU-China/Project?catalog=0"),
-
new picObj("https://static.igem.org/mediawiki/2012/3/37/Pfadr_picture.png", "PfadR: Synthesized promoter regulated solely by fatty acid", "With tandem FadR binding site, PfadR is able to be repressed solely by fatty acid.", "https://2012.igem.org/Team:WHU-China/Project?catalog=2#PfadR"),
+
new picObj("https://static.igem.org/mediawiki/2012/f/ff/Promoter_design.png", "Monitoring", "With two novel synthetic promoters, Pcar and PfadR, our E.coslim is able to sense, respond to the high calorie molecules and therefore monitor the health condition in human intestine.", "https://2012.igem.org/Team:WHU-China/Project?catalog=2#Pcar"),
-
           new picObj("https://static.igem.org/mediawiki/2012/0/09/%E8%83%96%E5%AD%90%E7%98%A6%E5%AD%90.png", "Long term efficiency of the E.coslim", "When viewed at macroscopic way, the E.coslim may have an advantage of long term efficiency,by regulating the human gut microbiota.And we have certified this by mathematical model", "https://2012.igem.org/Team:WHU-China/Project?catalog=2#PfadR")
+
new picObj("http://partsregistry.org/wiki/images/f/ff/%E5%85%A8%E6%99%AF%E5%8A%A0%E6%AD%BB%E4%BA%A11.png", "Becoming highly efficient-- Panorama of the All Devices", "Four novel devices-- Fatty Acid Degradation, Cellulose Synthesis, Colonization and Death were designed to ensure high efficiency respond to high- calorie molecule, making people slim!", "https://2012.igem.org/Team:WHU-China/Project?catalog=3"),
 +
           new picObj("https://static.igem.org/mediawiki/2012/0/09/%E8%83%96%E5%AD%90%E7%98%A6%E5%AD%90.png", "Long term efficiency of the E.coslim", "By mathematical model, we showed that when viewed at a macroscopic level, the E. coslim may achieve long term efficiency by modulating the composition of gut microbiota.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=2")
]
]
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new picObj("https://static.igem.org/mediawiki/igem.org/8/82/Pathway_FattyAcid.png", "Fatty Acid Degradation", "Feel free to eat meat since the fat is all degraded by our E.coslim!", "https://2012.igem.org/Team:WHU-China/Project?catalog=3"),
new picObj("https://static.igem.org/mediawiki/igem.org/8/82/Pathway_FattyAcid.png", "Fatty Acid Degradation", "Feel free to eat meat since the fat is all degraded by our E.coslim!", "https://2012.igem.org/Team:WHU-China/Project?catalog=3"),
new picObj("https://static.igem.org/mediawiki/igem.org/1/18/Pathway_Cellulose.png", "Cellulose Synthesis", "Transforming glucose into cellulose, the high-sugar food you eat will be turned into vegetables!", "https://2012.igem.org/Team:WHU-China/Project?catalog=4"),
new picObj("https://static.igem.org/mediawiki/igem.org/1/18/Pathway_Cellulose.png", "Cellulose Synthesis", "Transforming glucose into cellulose, the high-sugar food you eat will be turned into vegetables!", "https://2012.igem.org/Team:WHU-China/Project?catalog=4"),
-
new picObj("https://static.igem.org/mediawiki/2012/e/ea/Device_three.png", "Colonization", "The magic molecule c-di-GMP enables E.coslim colonizing in the gut to better fulfill its magic function!", "https://2012.igem.org/Team:WHU-China/Project?catalog=5")
+
new picObj("https://static.igem.org/mediawiki/2012/e/ea/Device_three.png", "Colonization", "The magic molecule c-di-GMP enables E.coslim colonizing in the gut to better fulfill its magic function!", "https://2012.igem.org/Team:WHU-China/Project?catalog=5"),
 +
new picObj("https://static.igem.org/mediawiki/2012/b/b6/DEATH.png", "Death", "We plan to use xylose inducing death device to hlep people terminate the effect of E. coslim at will as well as prevent horizontal gene transfer", "https://2012.igem.org/Team:WHU-China/Project?catalog=6")
]
]
},
},
 +
{
{
title : "<strong>Future Perspective</strong>",
title : "<strong>Future Perspective</strong>",
picArray : [
picArray : [
-
new picObj("https://static.igem.org/mediawiki/2012/a/ab/%E5%8F%8C%E8%83%B6%E5%9B%8A2.jpg", "Two Capsule Design", "Capsule I, the E.coslim, makes you slim, while Capsule II, xylose, terminates the effects of the former.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=0"),
+
new picObj("https://static.igem.org/mediawiki/2012/a/ab/%E5%8F%8C%E8%83%B6%E5%9B%8A2.jpg", "Two Capsule Design", "Capsule I contains the E.coslim, making people slim, while Capsule II contains xylose, terminating the effects of the Capsule I.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=0"),
new picObj("https://static.igem.org/mediawiki/2012/d/d2/Fpbody2.jpg", "In vitro experiment in a simulated intestine", "The gut microbiota and E.coslim will be inoculated in a simulated intestine, then the concentration measurement of each component will tell us whether E.coslim works as desired.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=0"),
new picObj("https://static.igem.org/mediawiki/2012/d/d2/Fpbody2.jpg", "In vitro experiment in a simulated intestine", "The gut microbiota and E.coslim will be inoculated in a simulated intestine, then the concentration measurement of each component will tell us whether E.coslim works as desired.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=0"),
-
new picObj("https://static.igem.org/mediawiki/2012/8/85/%E5%B0%8F%E9%BC%A0%E5%AE%9E%E9%AA%8C02.jpg", "In vivo experiment in mice", "E.coslim will also be inoculated into mice and the culture-independent microbiome analysis will tell us the real situation in vivo.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=0")
+
new picObj("https://static.igem.org/mediawiki/2012/8/85/%E5%B0%8F%E9%BC%A0%E5%AE%9E%E9%AA%8C02.jpg", "In vivo experiment in mice", "E.coslim will also be inoculated into mice and the microbiome analysis will tell us the real situation in vivo.", "https://2012.igem.org/Team:WHU-China/Modeling?catalog=0")
]
]
},
},
 +
 +
 +
{
{
-
title : "<strong>Achievement</strong>",
+
title : "<strong>Achievements</strong>",
picArray : [
picArray : [
 +
new picObj("https://static.igem.org/mediawiki/2012/d/d1/Achievements_device_xia.png", "Devices", "This year, we got solid results in every device we designed. In all 66 new biobricks we submitted, 39 of them have been proved to be effective. Our project also foucused on the cutting edge science such as c-di-GMP and gut microbiota ", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=0"),
 +
 +
new picObj("https://static.igem.org/mediawiki/2012/c/ca/Achievements_1.png", "Biobicks we have improved", "In our project, we have improved the function of some biobricks,such as BBa_J54222, BBa_K188011 and BBa_R0051. ", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=0"),
 +
 +
new picObj("https://static.igem.org/mediawiki/2012/f/f5/Achievements_2.png", "Novel promoter we designed", "As the existing promoter can not meet our demand, we designed two novel synthetic promoters Pcar and PfadR to achieve the goal of sensing and responding solely to high- calorie molecules. In addition, we also set up a standard for promoter design.", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=0"),
 +
 +
new picObj("https://static.igem.org/mediawiki/2012/2/27/Methods_we_improve.png", "Methods we improved", "In our project, we improved some traditional methods to measure the comsumption of fatty acids and production of cellulose. Also we developed a software named FANCY to detect the fluorescence intensity of single cell. ", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=0"),
 +
 +
new picObj("https://static.igem.org/mediawiki/2012/7/79/Human_practice_xia.png", "Human Practice", "We have helped HUST iGEM team debug their project and mathematical model. We also did a survey on GMOs and publicized iGEM to the public.", "https://2012.igem.org/Team:WHU-China/Notebooks?catalog=0"),
]
]
Line 56: Line 71:
Team : [
Team : [
{
{
 +
title : '<strong>Team Introduction</strong>',
 +
dir : []
 +
},
 +
                {
title : '<strong>Members</strong>',
title : '<strong>Members</strong>',
dir : [
dir : [
{
{
-
title : 'aaaa',
+
title : 'Jiawei Huang',
-
href : '#aaaa'
+
href : '#Jiawei Huang'
 +
},
 +
{
 +
title : 'Kuanwei Sheng',
 +
href : '#Kuanwei Sheng'
 +
},
 +
{
 +
title : 'Xian Xia',
 +
href : '#Xian Xia'
 +
},
 +
{
 +
title : 'Long Mei',
 +
href : '#Long Mei'
 +
},
 +
{
 +
title : 'Fan Cheng',
 +
href : '#Fan Cheng'
 +
},
 +
{
 +
title : 'Wenxiong Zhou',
 +
href : '#Wenxiong Zhou'
 +
},
 +
{
 +
title : 'Boxiang Wang',
 +
href : '#Boxiang Wang'
 +
},
 +
{
 +
title : 'Tong Wang',
 +
href : '#Tong Wang'
 +
},
 +
{
 +
title : 'Jiani Hu',
 +
href : '#Jiani Hu'
 +
},
 +
{
 +
title : 'Shiwei Gao',
 +
href : '#Shiwei Gao'
 +
},
 +
{
 +
title : 'Jing Zhang',
 +
href : '#Jing Zhang'
 +
},
 +
                        {
 +
title : 'Yuan Feng',
 +
href : '#Yuan Feng'
 +
},
 +
 
 +
{
 +
title : 'Pengcheng Zhou',
 +
href : '#PengchengZhou'
 +
},
 +
{
 +
title : 'Chang Liu',
 +
href : '#Chang Liu'
 +
},
 +
{
 +
title : 'Min Ye',
 +
href : '#Min Ye'
 +
},
 +
{
 +
title : 'Xuepeng Huang',
 +
href : '#Xuepeng Huang'
 +
},
 +
{
 +
title : 'Yancheng Liu',
 +
href : '#Yancheng Liu'
 +
},
 +
{
 +
title : 'Cheng Zhao',
 +
href : '#Cheng Zhao'
 +
},
 +
{
 +
title : 'Chao Huang',
 +
href : '#Chao Huang'
 +
},
 +
                        {
 +
title : 'Yutian Zhang',
 +
href : '#Yutian Zhang'
 +
},
 +
 
 +
{
 +
title : 'Wei Hong',
 +
href : '#Wei Hong'
 +
},
 +
{
 +
title : 'Liuyi Hu',
 +
href : '#Liuyi Hu'
 +
                                                },
 +
{
 +
title : 'Jin Liu',
 +
href : '#Jin Liu'
}
}
-
]
+
      ]
},
},
{
{
Line 68: Line 177:
dir : [
dir : [
{
{
-
title : 'bbbb',
+
title : 'Jianbo Wang',
-
href : '#bbbb'
+
href : '#Jianbo Wang'
-
}
+
},
 +
                                                {
 +
                                                        title : 'Zhixiong Xie',
 +
                                                        href : '#Zhixiong Xie'
 +
                                                },
 +
                                                {
 +
                                                        title : 'Yu Chen',
 +
                                                        href : '#Yu Chen'
 +
                                                }
]
]
},
},
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},
},
{
{
-
title : '<strong>Acknow-</br>ledgements</strong>',
+
title : '<strong>Acknow-</br>ledgements & Attributions</strong>',
-
dir : []
+
dir : [
-
},
+
    {
 +
title : 'Acknowledgements',
 +
href : '#Acknowledgements'
 +
},
 +
                        {
 +
                            title : 'Attributions',
 +
                            href : '#Attributions'
 +
                        },
 +
]
 +
}
],
],
-
Project : [
+
Project: [{
-
{
+
title: '<strong>Description</strong>',
-
title : '<strong>Description</strong>',
+
dir: []
-
dir : [
+
-
+
-
]
+
},
},
{
{
-
title : '<strong>Background</strong>',
+
title: '<strong>Background</strong>',
-
dir : [
+
dir: [
-
{
+
 
-
title : 'bbbb',
+
-
href : '#bbbb'
+
-
}
+
]
]
},
},
{
{
-
title : '<strong>Promoter Design</strong>',
+
title: '<strong>Promoter Design</strong>',
-
dir : []
+
dir: [{
 +
title: 'Introduction',
 +
href: '#Introduction'
 +
},{
 +
title: 'Indirect Pathway Design',
 +
href: '#Indirect Pathway Design'
 +
},
 +
{
 +
title: 'Direct Regulatory Promoter Design',
 +
href: '#Direct Regulatory Promoter Design'
 +
},
 +
{
 +
title: '<i>Pcar</i>',
 +
href: '#Pcar'
 +
},
 +
{
 +
title: '<i>PfadR</i>',
 +
href: '#PfadR'
 +
},
 +
{
 +
title: 'Standard',
 +
href: '#Standard'
 +
}]
},
},
{
{
-
title : '<strong>Device I: Fatty Acid Degradation</strong>',
+
title: '<strong>Device I: Fatty Acid Degradation</strong>',
-
dir : []
+
dir: [
 +
{
 +
    title: 'Purpose',
 +
href: '#Purpose'
 +
},
 +
{
 +
    title: 'Outline',
 +
    href: '#Outline'
 +
},
 +
{
 +
    title: 'Progress',
 +
    href: '#Progress'
 +
},
 +
{
 +
    title: 'Experimental Procedure',
 +
    href: '#Experimental Procedure'
 +
},
 +
{
 +
    title: 'Results',
 +
    href: '#Results'
 +
}
 +
]
},
},
{
{
-
title : '<strong>Device II: Cellulose Synthesis</strong>',
+
title: '<strong>Device II: Cellulose Synthesis</strong>',
-
dir : []
+
dir: [{
 +
    title: 'Outline',
 +
href: '#Outline1'
 +
},
 +
{
 +
    title: 'Design',
 +
href: '#Design1'
 +
},
 +
{
 +
    title: 'Progress',
 +
href: '#Progress1'
 +
},
 +
{
 +
    title: 'Results',
 +
href: '#Results1'
 +
},{
 +
    title: 'Discussion',
 +
href: '#Discussion1'
 +
},]
},
},
{
{
-
title : '<strong>Device III: Colonization</strong>',
+
title: '<strong>Device III: Colonization</strong>',
-
dir : []
+
dir: [
 +
{
 +
    title: 'Purpose',
 +
href: '#Purpose2'
 +
},
 +
{
 +
    title: 'Outline',
 +
    href: '#Outline2'
 +
},
 +
{
 +
    title: 'Experimental Procedure',
 +
    href: '#Experimental Procedure2'
 +
},
 +
{
 +
    title: 'Results',
 +
    href: '#Results2'
 +
}
 +
]
},
},
-
],
+
{
 +
title: '<strong>Device IV: Death</strong>',
 +
dir: [{
 +
    title: 'Outline',
 +
    href: '#Outline4'
 +
},
 +
{
 +
    title: 'Description',
 +
    href: '#Description'
 +
},
 +
 +
{
 +
    title: 'Prevention of HGT',
 +
    href: '#Prevention of HGT'
 +
}]
 +
},
 +
 
 +
],
Modeling : [
Modeling : [
{
{
-
title : '<strong>Future Perspectives</strong>',
+
title : '<strong>Future Perspective</strong>',
dir : []
dir : []
},
},
Line 134: Line 349:
dir : [
dir : [
{
{
-
title : 'aaaa',
+
title : 'The Ordinary Differential Equations of the Model',
-
href : '#aaaa'
+
href : '#The Ordinary Differential Equations of the Model'
 +
},
 +
{
 +
title : 'Analysis on the Steady State of the ODE',
 +
href : '#Analysis on the Steady State of the ODE'
 +
},
 +
{
 +
title : 'Parameter Screening',
 +
href : '#Parameter Screening'
 +
},
 +
{
 +
title : 'Conclusion',
 +
href : '#Conclusion'
}
}
]
]
Line 143: Line 370:
dir : [
dir : [
{
{
-
title : 'bbbb',
+
title : 'Gut Microbiota Regulation',
-
href : '#bbbb'
+
href : '#Gut Microbiota Regulation'
 +
},
 +
{
 +
title : 'Situation Before the Establishment of <i>E. coslim</i> Flora',
 +
href : '#Situation Before the Establishment of <i>E. coslim</i> Flora'
 +
},
 +
{
 +
title : 'Situation After the Establishment of <i>E. coslim</i> Flora',
 +
href : '#Situation After the Establishment of <i>E. coslim</i> Flora'
 +
},
 +
{
 +
    title : 'Conclusion',
 +
href : '#Conclusion1'
}
}
]
]
 +
},
},
{
{
title : '<strong>Software Tool: FANCY</strong>',
title : '<strong>Software Tool: FANCY</strong>',
-
dir : []
+
dir : [
 +
{
 +
title : 'Abstract',
 +
href : '#Abstract'
 +
},
 +
{
 +
title : 'Protocol',
 +
href : '#Protocol'
 +
},
 +
{
 +
title : 'Performance Evaluation',
 +
href : '#Performance Evaluation'
 +
},
 +
{
 +
title : 'Source Code',
 +
href : '#Source Code'
 +
}
 +
                                              ]
}
}
],
],
Notebooks : [
Notebooks : [
 +
                {
 +
title : '<strong>Achievements</strong>',
 +
dir : []
 +
},
{
{
title : '<strong>Parts</strong>',
title : '<strong>Parts</strong>',
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dir : [
dir : [
{
{
-
title : 'aaaa',
+
title : 'Brief Answers to Safety Questions',
-
href : '#aaaa'
+
href : '#Brief Answers to Safety Questions'
 +
},
 +
{
 +
    title : 'General Safety Issues',
 +
href : '#General Safety Issues'
 +
},
 +
{
 +
    title : 'Safety Considerations of Our Biobrick parts and Our Project',
 +
href : '#Safety Considerations of Our Biobrick parts and Our Project'
 +
},
 +
{
 +
    title : 'Safety Management and Practice',
 +
href : '#Safety Management and Practice'
 +
},
 +
{
 +
    title : 'Biosafety and Publicity',
 +
href : '#Biosafety and Publicity'
}
}
]
]
Line 171: Line 448:
dir : [
dir : [
{
{
-
title : 'bbbb',
+
title : 'Protocol for Fluorescence Measurement',
-
href : '#bbbb'
+
href : '#Protocol for fluorescence measurement'
 +
},
 +
{
 +
title : 'Protocol of Cupric-Soap Reaction',
 +
href : '#Protocols of Cupric-Soap Reaction'
 +
},
 +
{
 +
title : 'Krebs-Ringer Phosphate（KRPD Buffer Solution）',
 +
href : '#Krebs-Ringer Phosphate（KRPD Buffer Solution）'
 +
},
 +
                        {
 +
title : 'Cellulose Detection',
 +
href : '#Cellulose Detection'
 +
},
 +
 +
{
 +
title : '<i>In vitro</i> Experiment',
 +
href : '#In vitro Experiment'
 +
},
 +
                        {
 +
title : 'Plate Assay',
 +
href : '#Plate Assay'
 +
},
 +
{
 +
title : 'Materials and Methods of SDS-PAGE',
 +
href : '#Materials and Methods of SDS-PAGE'
}
}
]
]
Line 181: Line 483:
},
},
                                 {
                                 {
-
                                         title : '<strong>Brain-</br>storming</strong>',
+
                                         title : '<strong>Brainstorming</strong>',
                                         dir : []
                                         dir : []
                                 },
                                 },
{
{
title : '<strong>Gallery</strong>',
title : '<strong>Gallery</strong>',
 +
dir : [
 +
    {
 +
title : 'Team',
 +
href : '#team'
 +
},
 +
{
 +
title : 'Presentation',
 +
href : '#presentation'
 +
},
 +
{
 +
title : 'Human Practice',
 +
href : '#human practice'
 +
},
 +
{
 +
title : 'Doodle',
 +
href : '#doodle'
 +
},
 +
{
 +
title : 'Funny Pictures',
 +
href : '#funny pictures'
 +
}]},
 +
                {
 +
title : '<strong>A Letter from Fancy</strong>',
dir : []
dir : []
 +
 +
}
}
],
],
Line 192: Line 519:
{
{
title : '<strong>Outline</strong>',
title : '<strong>Outline</strong>',
-
dir : [
+
                                    dir : []
-
{
+
-
title : 'aaaa',
+
-
href : '#aaaa'
+
-
}
+
-
]
+
},
},
{
{
title : '<strong>Questionaire Survey</strong>',
title : '<strong>Questionaire Survey</strong>',
-
dir : [
+
    dir : [{
-
{
+
title : 'Background',
-
title : 'bbbb',
+
href : '#Background'
-
href : '#bbbb'
+
},{
-
}
+
title : 'Methods',
-
]
+
href : '#Methods'
 +
},{
 +
title : 'Results',
 +
href : '#Results'
 +
},{
 +
title : 'Discussion',
 +
href : '#Discussion'
 +
}]
},
},
{
{
title : '<strong>Publicity</strong>',
title : '<strong>Publicity</strong>',
-
dir : []
+
    dir : []
}
}
-
]
+
] };
-
};
+
//*************************自定义数据部分*************************
//*************************自定义数据部分*************************
</script>
</script>

Latest revision as of 04:01, 27 October 2012

  •

                Retrieved from "http://2012.igem.org/Team:WHU-China"