Team:Penn/Parts

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<b><div class="name" align="center">Parts Submitted</div></b><br>
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<b><div class="name">INPNC-MCS (Multiple Cloning Site): Winner of "Best BioBrick, Engineered" at Americas East</div></b>
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This is a template page. READ THESE INSTRUCTIONS.
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A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p>
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<b><div class="name">ClyA</div></b>
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a>  </p>
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<b><div class="name">His-ClyA</div></b>
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You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified.   PLEASE keep all of your pages within your teams namespace.
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<p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811001"> BBa_K811001 </a></p>
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<b><div class="name">ClyA-His</div></b>
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<p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811002"> BBa_K811002 </a></p>
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<b><div class="name">INPNC</div></b>
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<p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811003"> BBa_K811003 </a></p>
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<b><div class="name">INPNC-HA</div></b>
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<p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811004"> BBa_K811004 </a></p>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:Penn|Home]]
 
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!align="center"|[[Team:Penn/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Penn Official Team Profile]
 
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!align="center"|[[Team:Penn/Project|Project]]
 
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!align="center"|[[Team:Penn/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Penn/Modeling|Modeling]]
 
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!align="center"|[[Team:Penn/Notebook|Notebook]]
 
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!align="center"|[[Team:Penn/Safety|Safety]]
 
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!align="center"|[[Team:Penn/Attributions|Attributions]]
 
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
 
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Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
 
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<groupparts>iGEM012 Penn</groupparts>
 

Latest revision as of 03:59, 27 October 2012

Penn 2012 iGEM Wiki

Image Map

Parts Submitted

INPNC-MCS (Multiple Cloning Site): Winner of "Best BioBrick, Engineered" at Americas East
 A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See BBa_K811005


ClyA

Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See BBa_K811000


His-ClyA

A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See BBa_K811001


ClyA-His

A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See BBa_K811002


INPNC

A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See BBa_K811003


INPNC-HA

A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See BBa_K811004


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