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Team:SJTU-BioX-Shanghai
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<a href="/Team:SJTU-BioX-Shanghai/Project/project2.2"><div id="entry-cover1"> | <a href="/Team:SJTU-BioX-Shanghai/Project/project2.2"><div id="entry-cover1"> | ||
- | <p>Membrane Accelerator could help substrates flow by decreasing distance that intermediates have to travel between enzymes. Besides, restricted reaction space on membrane could increase the local products | + | <p>Membrane Accelerator could help substrates flow by decreasing distance that intermediates have to travel between enzymes. Besides, restricted reaction space on membrane could increase the local products concen- tration near membrane, thus to facilitate the exportation of final products </p> |
- | <p>We successfully increased the yield of fatty acids by 24 fold through building a fatty-acid-producing | + | <p>We successfully increased the yield of fatty acids by 24 fold through building a fatty-acid-producing Mem- brane Accelerator.</p> |
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<a href="/Team:SJTU-BioX-Shanghai/Project/project2.3"><div id="entry-cover2"> | <a href="/Team:SJTU-BioX-Shanghai/Project/project2.3"><div id="entry-cover2"> | ||
- | <p>Previous synthetic scaffold | + | <p>Previous synthetic scaffold con- trolling metabolic flux all focused on biosynthesis. Now we propose a new direction in the application of syn- thetic scaffold: accelerating biodegradation pathways. </p> |
- | <p>Natural biodegradation is a very slow process but indispensible in environment restoration. Membrane Accelerator is expected to increase rate of | + | <p>Natural biodegradation is a very slow process but indispensible in environment restoration. Membrane Accelerator is expected to increase rate of biodegrada- tion pathway sharply due to its multiple privileges.</p> |
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<a href="/Team:SJTU-BioX-Shanghai/Project/project2.1"><div id="entry-cover3"> | <a href="/Team:SJTU-BioX-Shanghai/Project/project2.1"><div id="entry-cover3"> | ||
- | <p>Dynamically and artificially regulating the direction of biochemical pathway has remained a challenge to all. Based on Membrane Scaffold, we achieved this goal through controlling aggregation state of crucial enzymes in branched reactions. This novel device is called Membrane Rudder.</p> | + | <p>Dynamically and artificially regulating the direction of biochemical pathway has remained a challenge to all. Based on Membrane Scaffold, we achieved this goal through controlling aggregation state of crucial enzymes in branched reactions with light. This novel device is called Membrane Rudder.</p> |
<p>We then further connected the post-translational control system (Membrane Rudder) to genetic circuits and expanded its potential signal pool.</p> | <p>We then further connected the post-translational control system (Membrane Rudder) to genetic circuits and expanded its potential signal pool.</p> | ||
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- | <p><strong>< | + | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"> The honors we have won:</font></strong> Regional Winner, Best New BioBrick Part or Device Engineered and Gold Medal at Asia Jamboree; Advanced to World Championship.</p> |
- | <p><strong>< | + | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"> The breakthrough we made:</font></strong> Redefinition of scaffold in Synthetic Biology by recruiting <i>E.coli’</i>s inner membrane as a natural two-dimensional scaffold. </p> |
- | <p><strong>< | + | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"> The system we built:</font></strong> 6 membrane proteins orderly organized on the inner membrane of <i>E.coli</i>, the feasibility of which has been confirmed by fluorescence complementation assay and biosynthesis experiment.</p> |
- | <p><strong>< | + | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"> Device 1 we created – Membrane Accelerator:</font></strong> A universal tool that serves to accelerate biochemical reactions in <i>E.coli</i>; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type <i>E.coli </i>and 9 fold compared to <i>E.coli</i> overexpressing cytoplasmic enzymes.</p> |
- | <p><strong>< | + | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"> Device 2 we created – Membrane Rudder:</font></strong> A universal tool used to dynamically and artificially control biochemical reactions in <i>E.coli</i>; the direction of Violacein and Deoxyviolacein synthetic pathway was successfully switched through changing blue light signal. </p> |
- | <p><strong>< | + | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"> New direction we proposed:</font></strong> The application of scaffold system in accelerating biodegradation pathway by using Membrane Accelerator.</p> |
- | <p><strong>< | + | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"> Parts we submitted:</font></strong> 42 well-characterized parts that could either be used directly or serve as a universal tool readily for potential scientific or engineering use.</p> |
- | <p><strong>< | + | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"> A club we established – BioCraft:</font></strong> The headquarter of our human practice programs, having come a long way in propagandizing Synthetic Biology and iGEM. Warmly-received activities have been held in and outside the campus. Several celebrities in different fields have shown support for us, laying a cornerstone for our future development.</p> |
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<img src="/wiki/images/b/ba/12SJTU_home_Team.jpg"></img> | <img src="/wiki/images/b/ba/12SJTU_home_Team.jpg"></img> | ||
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- | < | + | <iframe width="480" height="400" src="http://www.youtube.com/embed/PkE9MtApxAE" frameborder="0" allowfullscreen></iframe> |
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Latest revision as of 03:58, 27 October 2012
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