Team:Uppsala University/Results

From 2012.igem.org

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<h2>sRNA gene silencing</h2>
<h2>sRNA gene silencing</h2>
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<div id="desc">
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<p>We have developed a modular screening system and protocol for finding silencing sRNA:s against arbitrary genes. Using this, we have found a strongly silencing sRNA:s against a clinical antibiotic gene and lowered the minimary inhibatory concentration five-fold in resistant bacteria.  
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<p>We have developed a modular screening system and protocol for finding silencing sRNAs against arbitrary genes. Using this, we have found strongly silencing sRNAs against a clinical antibiotic gene and lowered the minimal inhibatory concentration ten-fold in resistant bacteria.  
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<h2>New standard backbone series</h2>
<h2>New standard backbone series</h2>
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<p>We have constructed a range of new standard low copy backbones, and variants with built-in lacIq repression for tight control of toxic genes, thermosensitivity and FRT sites for removing resistance cassettes. This work was induced as it turned out that the common registry pSB4 backbones all have a faulty copy number regulation, while we needed low copy backbones for out project.</p>
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<p>We have constructed a range of new standard low copy backbones, and variants with built-in lacIq repression for tight control of toxic genes, thermosensitivity and FRT sites for removing resistance cassettes. This work was as it turned out that the common registry pSB4 backbones all have faulty copy number regulation, while we needed low copy backbones for out project.</p>
<p id="more"><a href="/Team:Uppsala_University/Backbones">Read more...</a></p>
<p id="more"><a href="/Team:Uppsala_University/Backbones">Read more...</a></p>
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<h2>Chromoproteins</h2>
<h2>Chromoproteins</h2>
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<p id="second">Proteins with an visible intrinsic color are the simplest possible reporters i molecular biology. Most iGEM:ers are familiar with the Red Flourescent Protein (RFP), but there are many other colors aviable among all organism of the world. We have characterized and submitted new chromoproteins, allowing multiplexed colorful reporters. </p>
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<p id="second">Proteins with a visible intrinsic color are the simplest possible reporters i molecular biology. Most iGEMers are familiar with the Red Flourescent Protein (RFP), but there are many other colors available among the organisms of the world. We have characterized and submitted new chromoproteins, allowing multiplexed colorful reporters. </p>
<p id="more"><a href="/Team:Uppsala_University/Chromoproteins">Read more...</a></div>
<p id="more"><a href="/Team:Uppsala_University/Chromoproteins">Read more...</a></div>
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<h2>Scarless gene deletion</h2>
<h2>Scarless gene deletion</h2>
<div id="desc">
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<p id="second">In our project we have extensivly used recombineering onto the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides  easy and reliable recombineering, and the possibility of scarless gene deletion. </p>
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<p id="second">In our project we have extensivly used homology recombineering into the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides  easy and reliable recombineering, and the possibility of scarless gene deletion. </p>
<p id="more"><a href="/Team:Uppsala_University/Catsac">Read more...</a></div>
<p id="more"><a href="/Team:Uppsala_University/Catsac">Read more...</a></div>
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Latest revision as of 02:25, 27 October 2012

Team Uppsala University – iGEM 2012



sRNA gene silencing

We have developed a modular screening system and protocol for finding silencing sRNAs against arbitrary genes. Using this, we have found strongly silencing sRNAs against a clinical antibiotic gene and lowered the minimal inhibatory concentration ten-fold in resistant bacteria.

Read more...

New standard backbone series

We have constructed a range of new standard low copy backbones, and variants with built-in lacIq repression for tight control of toxic genes, thermosensitivity and FRT sites for removing resistance cassettes. This work was as it turned out that the common registry pSB4 backbones all have faulty copy number regulation, while we needed low copy backbones for out project.

Read more...
 

Promoter characterization

We have characterized promoter strengths of eight popular promoters with Fluorescense Activated Cell Sorting (FACS), putting syntetic and wild-type promoters on the same scale.

Read more...

Chromoproteins

Proteins with a visible intrinsic color are the simplest possible reporters i molecular biology. Most iGEMers are familiar with the Red Flourescent Protein (RFP), but there are many other colors available among the organisms of the world. We have characterized and submitted new chromoproteins, allowing multiplexed colorful reporters.

Read more...

Scarless gene deletion

In our project we have extensivly used homology recombineering into the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides easy and reliable recombineering, and the possibility of scarless gene deletion.

Read more...


Sponsors






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