Team:Uppsala University/Results

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<a href="#dna">Plasmid removal</a> | <a href="#transcriptional">Gene networks</a> | <a href="#translational">Silencing sRNA:d</a> | <a href="#vector">Delivery</a> | <a href="#sideprojects">Side projects</a>
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<a href="https://2012.igem.org/Team:Uppsala_University/Translational">sRNA Genes Silencing</a> | <a href="https://2012.igem.org/Team:Uppsala_University/Backbones">New standard backbone series</a> | <a href="https://2012.igem.org/Team:Uppsala_University/Promoters">Promoter characterization</a> | <a href="https://2012.igem.org/Team:Uppsala_University/Chromoproteins">Chromoproteins</a> | <a href="https://2012.igem.org/Team:Uppsala_University/catsac">Scarless Gene Deletion</a>
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<h2>sRNA gene silencing</h2>
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<p>We have developed a modular screening system and protocol for finding silencing sRNAs against arbitrary genes. Using this, we have found strongly silencing sRNAs against a clinical antibiotic gene and lowered the minimal inhibatory concentration ten-fold in resistant bacteria.
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<tr><td class="subtext"><h2>DNA level - Plasmid removal</h2></td>
 
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<h2>New standard backbone series</h2>
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<p>We have constructed a range of new standard low copy backbones, and variants with built-in lacIq repression for tight control of toxic genes, thermosensitivity and FRT sites for removing resistance cassettes. This work was as it turned out that the common registry pSB4 backbones all have faulty copy number regulation, while we needed low copy backbones for out project.</p>
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<p id="more"><a href="/Team:Uppsala_University/Backbones">Read more...</a></p>
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<tr><td class="subtext"><h2>Transcriptional level - Targeting gene networks</h2></td>
 
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<h2>Promoter characterization</h2>
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<p id="second">We have characterized promoter strengths of eight popular promoters with Fluorescense Activated Cell Sorting (FACS), putting syntetic and wild-type promoters on the same scale. </p>
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<p id="more"><a href="/Team:Uppsala_University/Promoters">Read more...</a></div>
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<p id="second">Proteins with a visible intrinsic color are the simplest possible reporters i molecular biology. Most iGEMers are familiar with the Red Flourescent Protein (RFP), but there are many other colors available among the organisms of the world. We have characterized and submitted new chromoproteins, allowing multiplexed colorful reporters. </p>
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<p id="second">In our project we have extensivly used homology recombineering into the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides  easy and reliable recombineering, and the possibility of scarless gene deletion. </p>
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<p><b>New standard backbones</b></p>
 
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<p>We have constructed a range of new standard backbones, especially low copy backbones and backbones with built-in lacIq repression for tight control of toxic genes. This work was induced as it turned out that the common registry pSB4 backbones all have a faulty copy number regulation, while we needed low copy backbones for out project. <a href="http://https://2012.igem.org/Team:Uppsala_University/Backbones"> Read more...</a></p>
 
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<p>In our project we have extensivly used recombineering onto the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides very easy and reliable recombineering and the possibility of scarless gene deletion. <a href="http://https://2012.igem.org/Team:Uppsala_University/Catsac"> Read more...</a></p>
 
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Latest revision as of 02:25, 27 October 2012

Team Uppsala University – iGEM 2012



sRNA gene silencing

We have developed a modular screening system and protocol for finding silencing sRNAs against arbitrary genes. Using this, we have found strongly silencing sRNAs against a clinical antibiotic gene and lowered the minimal inhibatory concentration ten-fold in resistant bacteria.

Read more...

New standard backbone series

We have constructed a range of new standard low copy backbones, and variants with built-in lacIq repression for tight control of toxic genes, thermosensitivity and FRT sites for removing resistance cassettes. This work was as it turned out that the common registry pSB4 backbones all have faulty copy number regulation, while we needed low copy backbones for out project.

Read more...
 

Promoter characterization

We have characterized promoter strengths of eight popular promoters with Fluorescense Activated Cell Sorting (FACS), putting syntetic and wild-type promoters on the same scale.

Read more...

Chromoproteins

Proteins with a visible intrinsic color are the simplest possible reporters i molecular biology. Most iGEMers are familiar with the Red Flourescent Protein (RFP), but there are many other colors available among the organisms of the world. We have characterized and submitted new chromoproteins, allowing multiplexed colorful reporters.

Read more...

Scarless gene deletion

In our project we have extensivly used homology recombineering into the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides easy and reliable recombineering, and the possibility of scarless gene deletion.

Read more...


Sponsors






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