"
Team/CINVESTAV-IPN-UNAM MX/Oxigen Response.htm
From 2012.igem.org
| (5 intermediate revisions not shown) | |||
| Line 5: | Line 5: | ||
<style type="text/css"> | <style type="text/css"> | ||
| + | |||
| + | #top-section { | ||
| + | position: relative; | ||
| + | height: 20px; | ||
| + | width: 975px; | ||
| + | margin: 0 auto 0 auto; | ||
| + | border-left: hidden; | ||
| + | border-right: hidden; | ||
| + | border-bottom: hidden; | ||
| + | } | ||
* { | * { | ||
| Line 289: | Line 299: | ||
<div id="context"> | <div id="context"> | ||
| - | <h1><em>Oxygen Control System: PrrA/PrrB two component regulation system! </em></h1 | + | <h1><em>Oxygen Control System: PrrA/PrrB two <br /><br />component regulation system! </em></h1> |
| - | + | <p>This regulatory system is able to sense oxygen concentration and send a response, under high oxygen tension, the system remains inactive, when oxygen concentration decreases PrrB (Histidine sensor kinase) turns active through an autophosphorylation with help of PrrC, which receive the signal from electron transport chain. Then PrrB transfer a phosphate group to PrrA that directly binds promoter and recruits RNA polymerase to start GFP transcription. | |
| - | <p> | + | |
| - | through an autophosphorylation | + | |
| - | phosphate group to PrrA | + | (See the next video for a visual explanation).</p> |
| - | transcription. (See the next video for a visual explanation).</p> | + | |
<div align="center"><iframe width="480" height="360" src="http://www.youtube.com/embed/pgp6DzpNyA0" frameborder="0" allowfullscreen></iframe></div><br> | <div align="center"><iframe width="480" height="360" src="http://www.youtube.com/embed/pgp6DzpNyA0" frameborder="0" allowfullscreen></iframe></div><br> | ||
<p id="text2">Our biobricks </p> | <p id="text2">Our biobricks </p> | ||
| Line 304: | Line 313: | ||
<p align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Rodo05.jpg" alt="rodo05" width="450" height="249"></p> | <p align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Rodo05.jpg" alt="rodo05" width="450" height="249"></p> | ||
<p id="text2">We were inspired in:</p> | <p id="text2">We were inspired in:</p> | ||
| - | <p>This system is inspired in PrrBCA two component system from | + | <p>This system is inspired in PrrBCA two component system from <em>R. sphaeroides</em>, which is a master regulator involved in expression of |
approximately 850 genes, >20% of the genome (Kaplan & Eraso 2005) This system coordinately controls genes involved in the complex switch | approximately 850 genes, >20% of the genome (Kaplan & Eraso 2005) This system coordinately controls genes involved in the complex switch | ||
between aerobic and anaerobic conditions and the optimum use of reducing power. It also regulates gene expression involved in | between aerobic and anaerobic conditions and the optimum use of reducing power. It also regulates gene expression involved in | ||
| Line 313: | Line 322: | ||
<p id="refe">References<br> | <p id="refe">References<br> | ||
1. | 1. | ||
| - | Kaplan S, Eraso J, Roh JH. (2005). Interacting regulatory networks in the facultative | + | Kaplan S, Eraso J, Roh JH. (2005). <strong>Interacting regulatory networks in the facultative |
| - | photosynthetic bacterium, Rhodobacter sphaeroides 2.4.1. Biochem. Soc. Trans. 33:51–55<br> | + | photosynthetic bacterium, <em>Rhodobacter sphaeroides</em> 2.4.1.</strong> Biochem. Soc. Trans. 33:51–55<br> |
2. | 2. | ||
| - | Zeilstra-Ryalls JH, Kaplan S. (2004). Oxygen intervention in the regulation of gene xpression: the photosynthetic | + | Zeilstra-Ryalls JH, Kaplan S. (2004). <strong>Oxygen intervention in the regulation of gene xpression: the photosynthetic |
| - | bacterial paradigm. Cell. Mol. Life Sci. 61:417–36<br> | + | bacterial paradigm.</strong> Cell. Mol. Life Sci. 61:417–36<br> |
3. | 3. | ||
| - | Eraso JM, Kaplan S (2009) Regulation of gene expression by PrrA in Rhodobacter sphaeroides 2.4.1: role of | + | Eraso JM, Kaplan S (2009) <strong>Regulation of gene expression by PrrA in <em>Rhodobacter sphaeroides</em> 2.4.1: role of |
| - | polyamines and DNA topology. J Bacteriol 2009, 191(13):4341-4352. | + | polyamines and DNA topology.</strong> J Bacteriol 2009, 191(13):4341-4352. |
</p> | </p> | ||
| Line 342: | Line 351: | ||
<!-- end #page --> | <!-- end #page --> | ||
<div id="piedepagina"> | <div id="piedepagina"> | ||
| - | <p align="center"> Rhodofactory 2012 </p> | + | <p align="center"> <strong>Rhodofactory 2012</strong> </p> |
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/8/8a/Icytdf.png" alt="icytdf" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/8/8a/Icytdf.png" alt="icytdf" width="90" height="82" /></div> |
</div> | </div> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| Line 351: | Line 360: | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fb/Bio.png" alt="bio" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fb/Bio.png" alt="bio" width="80" height="97" /></div> |
</div> | </div> | ||
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png" alt="fermentAS" width="82" height="45" /></div> | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png" alt="fermentAS" width="82" height="45" /></div> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/a/a3/Cinestav.png" alt="cinestav" width="74" height=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/a/a3/Cinestav.png" alt="cinestav" width="74" height="91" /></div> |
</div> | </div> | ||
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div> | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width="83" height="93" /></div> |
</div> | </div> | ||
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/c/c9/Gto.png" alt="gto" width="84" height="46" /></div> | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/c/c9/Gto.png" alt="gto" width="84" height="46" /></div> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/Qimica.png" alt="quimica" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/Qimica.png" alt="quimica" width="80" height="72" /></div> |
</div> | </div> | ||
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/7/73/Valaner.png" alt="valaner" width="84" height="48" /></div> | <div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/7/73/Valaner.png" alt="valaner" width="84" height="48" /></div> | ||
<div id="sponsors"> | <div id="sponsors"> | ||
| - | <div align="center"><img src="https://static.igem.org/mediawiki/2012/7/79/Ipn.png" alt="ipn" width=" | + | <div align="center"><img src="https://static.igem.org/mediawiki/2012/7/79/Ipn.png" alt="ipn" width="70" height="96" /></div> |
</div> | </div> | ||
Latest revision as of 00:22, 27 October 2012
Oxygen Control System: PrrA/PrrB two
component regulation system!
This regulatory system is able to sense oxygen concentration and send a response, under high oxygen tension, the system remains inactive, when oxygen concentration decreases PrrB (Histidine sensor kinase) turns active through an autophosphorylation with help of PrrC, which receive the signal from electron transport chain. Then PrrB transfer a phosphate group to PrrA that directly binds promoter and recruits RNA polymerase to start GFP transcription. (See the next video for a visual explanation).
Our biobricks
The first biobrick consists in the complete oxygen dependent system, PrrA, PrrB and PrrC, each one with a Ribosome Binding Site, under a Medium strength promoter (J23104), this first biobrick also it has the PrrA dependent promoter and GFP as a reporter gene
The second biobrick is just the PrrA dependent promoter and GFP as a reporter gene.
We were inspired in:
This system is inspired in PrrBCA two component system from R. sphaeroides, which is a master regulator involved in expression of approximately 850 genes, >20% of the genome (Kaplan & Eraso 2005) This system coordinately controls genes involved in the complex switch between aerobic and anaerobic conditions and the optimum use of reducing power. It also regulates gene expression involved in photosynthesis, carbon dioxide fixation, nitrogen fixation, hydrogen uptake, aerotaxis, denitrification, electron transport, aerobic and anaerobic respiration, and heme biosynthesis, and others. Thus emphasizing its global role (Elsen et.al 2004, Kaplan & Eraso 2005, Zeilstra-Ryalls & Kaplan 2004).
References
1.
Kaplan S, Eraso J, Roh JH. (2005). Interacting regulatory networks in the facultative
photosynthetic bacterium, Rhodobacter sphaeroides 2.4.1. Biochem. Soc. Trans. 33:51–55
2.
Zeilstra-Ryalls JH, Kaplan S. (2004). Oxygen intervention in the regulation of gene xpression: the photosynthetic
bacterial paradigm. Cell. Mol. Life Sci. 61:417–36
3.
Eraso JM, Kaplan S (2009) Regulation of gene expression by PrrA in Rhodobacter sphaeroides 2.4.1: role of
polyamines and DNA topology. J Bacteriol 2009, 191(13):4341-4352.
Rhodofactory 2012
