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<li class="active"><a href="home.htm">Home</a></li>
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<li><a href="rhodofactory.htm">Rhodofactory</a></li>
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<a href="/Team/CINVESTAV-IPN-UNAM_MX/home.htm">Home<span class="flecha">&#9660;</span></a>
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<a href="#">Rhodofactory<span class="flecha">&#9660;</span></a>
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<h1><a href="#"></a></h1>  
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<h2><span></span></h2>
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<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Overview.htm">Overview<span class="flecha">&#9660;</span></a></li>
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</div>
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<!-- end #header -->
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            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Light Response.htm">Light Response<span class="flecha">&#9660;</span></a></li>
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            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Oxigen Response.htm">Oxigen Response<span class="flecha">&#9660;</span></a></li>
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<h1><em>Rhodofactory, controlling genetic expression: an oxygen <br /><br />and light response!</em></h1>
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<img src="https://static.igem.org/mediawiki/2012/e/ec/Mascota.jpg" width="290" height="287" class="left">
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</ul>
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<p>The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light,
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</li>
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darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking
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<li>
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advantage of this, our project aims to build two genetic control systems based on <em> Rhodobacter sphaeroides </em>
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<a href="">Results<span class="flecha">&#9660;</span></a>
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photosynthesis cluster regulation. The first one is a light dependent system controlled by two
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<ul>
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proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is
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<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Biobricks.htm">Biobricks<span class="flecha">&#9660;</span></a></li>
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an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on
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R. palustris chassis, using a cassete in which a reporter (GFP) is regulated by external conditions
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            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Notebook.htm">Notebook<span class="flecha">&#9660;</span></a></li>
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that activate or repress its expresion. Once we have characterized the functionality of these
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networks, our perspective is to develop a Rhodofactory, it means to control the produccion of
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            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/palustris response.htm">R. palustris response<span class="flecha">&#9660;</span></a></li>
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differents metabolites, such as biodiesel and butanol, using simple signals.</p>
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            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/sphaeroides response.htm">R. sphaeroides response<span class="flecha">&#9660;</span></a></li>
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<p align="center"> <strong>Rhodofactory 2012</strong></p>
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<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png" alt="fermentAS" width="82" height="45" /></div>
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      <p>The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on <em>R. sphaeroides</em> photosynthesis regulation. </p>
+
</div>
-
      <p>The first one is a light dependent system controlled by two proteins that works as an antirepresor/repressor mechanism, and the second one is an oxygen dependent two-component system. We tested these devices on <em>R. palustris</em>. We built a genetic circuit that activate or repress GFP expression in response to external conditions.</p>
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div>
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       <p> Once we characterize the functionality of these networks, we want to create a Rhodofactory to use our systems to control production butanol using simple signals. </p>
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Latest revision as of 00:20, 27 October 2012

Rho

Rhodofactory, controlling genetic expression: an oxygen

and light response!

The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on Rhodobacter sphaeroides photosynthesis cluster regulation. The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on R. palustris chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion. Once we have characterized the functionality of these networks, our perspective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals.

 

Rhodofactory 2012

icytdf
osli
bio
fermentAS
cinestav
genscript
unam
gto
quimica
valaner
ipn

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