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Team:Penn/LightActivatedOverview
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<b><div class="name" align="center">Objectives</div></b> | <b><div class="name" align="center">Objectives</div></b> | ||
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| - | <p style="color:black;text-indent:30px;">In order to develop a module for light activated cell lysis, we had to implement two | + | <p style="color:black;text-indent:30px;">In order to develop a module for light activated cell lysis, we had to implement two elements: |
| - | <ul><li> | + | <ul style="font-size:20px"><li><b>1) A light-activation system that can express a gene of interest</li> |
| - | <li> | + | <li>2) A cytotoxic protein that can be expressed as our therapeutic drug to lyse cancer sells</li> |
</ul> | </ul> | ||
</div> | </div> | ||
Revision as of 23:02, 26 October 2012
Light-Activated Cell Lysis
Objectives
In order to develop a module for light activated cell lysis, we had to implement two elements:
- 1) A light-activation system that can express a gene of interest
- 2) A cytotoxic protein that can be expressed as our therapeutic drug to lyse cancer sells
YF1/FixJ Characterization
After reading many papers to select an appropriate light-sensing system to use, we selected the YF1/FixJ blue light system. We had also considered the red light sensor Cph8 but ultimately decided on YF1/FixJ because of its high on/off ratio of gene expression and also because of its availability to us (we were fortunate enough to come across the YF1/FixJ system in the form of the pDawn plasmid from the Moglich lab in Germany).
