Team:Lyon-INSA/results

From 2012.igem.org

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Every year industries spend billions of dollars to get rid of the biofilms which develop in their installations. Indeed biofims are reponsible for :<br/><br/>
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A new generation of solution against biofilms in industry <br/>
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<li>Food spoilage or poisoning in the food industry<br/>
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<li>Pathogens' persistence and dispersal in health industry<br/>
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<li>Corrosion and diameter reduction of pipelines<br/><br/>
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Reduction of harmful chemical reagents in cleaning processes<br/><br/>
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At the moment, industries have some solutions at their disposal to limit the biofilm development but they have generaly an environmental impact and/or an important cost.<br/><br/>  
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Reduction of cleaning costs for industrialists <br/><br/>
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Biofilms are responsible for billions of dollars in production losses and treatment costs in the industry every year:<br/>
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Thus, we develop a new generation of solution against biofilms in industry, which allows to reduce : <br/><br/>
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food spoilage or poisoning in the food industry ;<br/>
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pathogens' persistence and dispersal in health industry ;<br/><br/>
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Assuming that the environment is over-saturated with harmful chemicals such as biocides, whose long-term health effects still have to be elucidated, there is a great need for novel solutions to reduce detrimental biofilm effects.<br/><br/>
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<li>The use of harmful chemical reagents in cleaning processes<br/>
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<li>The cleaning costs for industrialists<br/>
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<h2>PROJECT STRATEGY </h2>
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<h2><strong>PROJECT STRATEGY</strong> </h2>
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Engineered bacterial "torpedos" capable of infiltrating, destroying biofilms (<strong>KILL</strong>) and protecting the cleaned surfaces by either a surfactant coating (<strong>COAT </strong> option),  
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Our solution is based on engineered bacterial "torpedos" capable of infiltrating, destroying biofilms (<strong>KILL</strong>) and protecting the cleaned surfaces by either a surfactant coating (<strong>COAT</strong> option), or by establishment of a positive biofilm (<strong> STICK </strong> option).<br/>
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or by establishment of a positive biofilm (<strong> STICK </strong> option).<br/>
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<center><img src="https://static.igem.org/mediawiki/2012/c/cb/ModelBs.jpg" width = 450px/></center><br/>
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This tunneling activity of <i>Bacillus subtilis</i> swimmer cells enables to :
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<li>Facilitate the delivery of the active substances within the biofilm.</li>
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<li>Destroy the inacessible biofilms in pipes.</li><br/>
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<li>Inacessible biofilms in pipes should be destroyed </li>
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<li>Delivery of active substance within the biofilm should be facilitated by the tunneling activity of <i> Bacillus </i> swimmer cells</li>
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Moreover <i>Bacillus</i> strains are non pathogenic and do not cause equipment deteroriation by corrosion.
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<i>Bacillus</i> strains are:
 
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<li>non pathogenic </li>
 
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<li>do not cause equipment deteroriation by corrosion</li>
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To sum up, this biocide-alternative strategy provides a money-saving and an environementally friendly solution for the control of unwanted biofilm.<br/><br/>
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This biocide-alternative strategy provides an money-saving and environementally friendly solution for the control of unwanted biofilm<br/><br/>
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<strong> LYSOSTAPHIN</strong>  part BBa_K802000<br/><br/>
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<strong> LYSOSTAPHIN</strong>  part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802000" target="_blank"><font color="#664499"><b> BBa_K802000</b></font></a> <br/><br/>
<center><img src="https://static.igem.org/mediawiki/2012/f/f3/Lyso.jpg" width = 650px/></center><br/>
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<strong> DISPERSIN</strong>  part BBa_K802001<br/><br/>
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<strong> DISPERSIN</strong>  part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802001" target="_blank"><font color="#664499"><b> BBa_K802001</b></font></a> <br/><br/>
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<strong> SURFACTIN + BIOFILM</strong>  part BBa_K802009<br/><br/>
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<strong> SURFACTIN + BIOFILM</strong>  part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K802009" target="_blank"><font color="#664499"><b> BBa_K802009</b></font></a><br/><br/>
<center><img src="https://static.igem.org/mediawiki/2012/3/31/Coatstick.jpg" width = 650px/></center><br/>
<center><img src="https://static.igem.org/mediawiki/2012/3/31/Coatstick.jpg" width = 650px/></center><br/>

Latest revision as of 21:31, 26 October 2012

Context and Objectives



Every year industries spend billions of dollars to get rid of the biofilms which develop in their installations. Indeed biofims are reponsible for :

  • Food spoilage or poisoning in the food industry
  • Pathogens' persistence and dispersal in health industry
  • Corrosion and diameter reduction of pipelines

    At the moment, industries have some solutions at their disposal to limit the biofilm development but they have generaly an environmental impact and/or an important cost.

    Thus, we develop a new generation of solution against biofilms in industry, which allows to reduce :

  • The use of harmful chemical reagents in cleaning processes
  • The cleaning costs for industrialists





    • Click on the title to show/hide the text.

    PROJECT STRATEGY


    Our solution is based on engineered bacterial "torpedos" capable of infiltrating, destroying biofilms (KILL) and protecting the cleaned surfaces by either a surfactant coating (COAT option), or by establishment of a positive biofilm ( STICK option).



    This tunneling activity of Bacillus subtilis swimmer cells enables to :
  • Facilitate the delivery of the active substances within the biofilm.
  • Destroy the inacessible biofilms in pipes.

    • Moreover Bacillus strains are non pathogenic and do not cause equipment deteroriation by corrosion.

    To sum up, this biocide-alternative strategy provides a money-saving and an environementally friendly solution for the control of unwanted biofilm.

    KILL : DESTROY BIOFILM


    LYSOSTAPHIN part BBa_K802000





    DISPERSIN part BBa_K802001



    COMBINED EFFECT


    COAT or STICK : AVOID NEW BIOFILM FORMATION


    SURFACTIN + BIOFILM part BBa_K802009



    AVOID NEW BIOFILM FORMATION

    NEW RESULTS



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