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Team:Tokyo Tech/Experiment/PHB2
From 2012.igem.org
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We made P(3HB) sheets. To make the sheets, we cultured <I>E.coli</I> JM109 in erlenmeyer flasks at 37°C for 72h. Contact angle is an indicator to represent the strength of the water-repellent. The angle shows the physical properties, especially surface tension. | We made P(3HB) sheets. To make the sheets, we cultured <I>E.coli</I> JM109 in erlenmeyer flasks at 37°C for 72h. Contact angle is an indicator to represent the strength of the water-repellent. The angle shows the physical properties, especially surface tension. | ||
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[[File:tokyotech PHB6.png|600px|thumb|center|Fig2-2-4-4-1, P(3HB) sheet]] | [[File:tokyotech PHB6.png|600px|thumb|center|Fig2-2-4-4-1, P(3HB) sheet]] | ||
==Protocol== | ==Protocol== | ||
| - | [[https://2012.igem.org/Team:Tokyo_Tech/Projects/PHAs/index.htm# | + | [[https://2012.igem.org/Team:Tokyo_Tech/Projects/PHAs/index.htm#Application Go to the P(3HB) production page "Application"]] |
Latest revision as of 19:56, 26 October 2012
Making purified P(3HB) sheets
We made P(3HB) sheets. To make the sheets, we cultured E.coli JM109 in erlenmeyer flasks at 37°C for 72h. Contact angle is an indicator to represent the strength of the water-repellent. The angle shows the physical properties, especially surface tension. When contact angle of sheets is larger than 90°, from Young equation, the sheets would have more strong water-repellent by increasing real surface area. Contact angle of P(3HB) sheets is about 100° from literature data. [Protocol]
Protocol
[Go to the P(3HB) production page "Application"]
1. Move dried cells into an airtight container.
2. Add chloroform, at rate of 2mg dried cells for 1 ml chloroform.
3. Incubate and stir the chloroform solution for more than 72 hrs at 20~25℃.
4. Filter the chloroform solution.
5. Concentrate by evaporation.
6. Dropwise the solution in methanol.
7. Filter the polymer in methanol, and dry the polymer.
8. Add a little chloroform to dissolve the polymer.
9. Poured the chloroform solution into a Petri dish.
10. Dry at room temperature.
