Team:Berkeley/Project/Localization/PromoterLibrary

From 2012.igem.org

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<div class="col12" id = "spacer4"> </div> </div>
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<div class="row">
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<div class="col1" align = "justify">
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<div class="col1">
<a name="Promoter Optimization"></a>
<a name="Promoter Optimization"></a>
     <img src="https://static.igem.org/mediawiki/2012/2/2f/Project1_subheader3.jpg" width="980">
     <img src="https://static.igem.org/mediawiki/2012/2/2f/Project1_subheader3.jpg" width="980">
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</div> </div>
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<div class="col12" id = "spacer4"> </div>
<div class="row">
<div class="row">
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<div class="col12" id = "spacer4"> </div>
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<div class="col12" id = "spacer4"> </div> </div>
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<h2> Narrow pixel brightness range (25-1000): </h2>
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<h2> Wide pixel brightness range (0-4095): </h2>
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<div>
<div>
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<table class="altrowstable" id="alternatecolor" align="center">
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<table class="altrowstable" id="alternatecolor2" align="center">
<tr>
<tr>
<th></th>
<th></th>
<th> Vacuolar Membrane </th>
<th> Vacuolar Membrane </th>
<th> Actin </th>
<th> Actin </th>
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<th> Cellular Periphery </th>
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<th> Plasma Membrane</th>
<th> Nucleus </th>
<th> Nucleus </th>
</tr>
</tr>
<tr>
<tr>
<td> pTDH3 </td>
<td> pTDH3 </td>
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<td> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td>
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<td width="205px"> <a href="https://static.igem.org/mediawiki/2012/6/65/YRC020_nrw_TDH3_ZRC1.jpg"> <img src="https://static.igem.org/mediawiki/2012/6/65/YRC020_nrw_TDH3_ZRC1.jpg" width="200" > </td>
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<td>Our yeast cells did not grow. We believe that the over expression of the protein killed the cell. </td>
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<td width="205px"> This strain of yeast cells did not grow; the over expression of the protein killed the cells. </td>
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<td> <img src="https://2012.igem.org/File:YRC030_minmax_TDH3_CP.jpg" width="200"> </td>
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<td width="205px"> <a href="https://static.igem.org/mediawiki/2012/b/bc/YRC030_nrw_TDH3_CP.jpg"> <img src="https://static.igem.org/mediawiki/2012/b/bc/YRC030_nrw_TDH3_CP.jpg" width="200" > </td>
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<td>The nucleus was extremely bright with pRPL18B already, so we did not need to test with stronger promoter. </td>
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<td width="205px"> The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter. </td>
</tr>
</tr>
<tr>
<tr>
<td> pTEF1 </td>
<td> pTEF1 </td>
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<td>row 2</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/6/66/YRC021_nrw_TEF1_ZRC1.jpg">
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<td> Our yeast cells did not grow. We believe that the over expression of the protein killed the cell. </td>
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<img src="https://static.igem.org/mediawiki/2012/6/66/YRC021_nrw_TEF1_ZRC1.jpg" width="200" > </td>
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<td>row 2</td>
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<td> This strain of yeast cells did not grow; the over expression of the protein killed the cells. </td>
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<td>row 2</td>
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<td><a href="https://static.igem.org/mediawiki/2012/4/42/YRC031_nrw_TEF1_CP.jpg"> <img src="https://static.igem.org/mediawiki/2012/4/42/YRC031_nrw_TEF1_CP.jpg" width="200" > </td>
 +
<td><a href="https://static.igem.org/mediawiki/2012/d/dc/YRC036_nrw_TEF1_Nucleus.jpg"> <img src="https://static.igem.org/mediawiki/2012/d/dc/YRC036_nrw_TEF1_Nucleus.jpg" width="200" > </td>
</tr>
</tr>
 +
<tr>
<tr>
<td> pRPL18B </td>
<td> pRPL18B </td>
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<td>row 3</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/b/b9/YVY015_nrw_RPL18B_ZRC1.jpg" >
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<td> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td>
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<img src="https://static.igem.org/mediawiki/2012/b/b9/YVY015_nrw_RPL18B_ZRC1.jpg" width="200" >  </td>
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<td>row 3</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/0/05/YTC03_nrw_RPL18B_Actin.jpg" >
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<td>row 3</td>
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<img src="https://static.igem.org/mediawiki/2012/0/05/YTC03_nrw_RPL18B_Actin.jpg" width="200" > </td>
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<td> <a href="https://static.igem.org/mediawiki/2012/5/59/YAJ12_nrw_RPL18B_CP.jpg">
 +
<img src="https://static.igem.org/mediawiki/2012/5/59/YAJ12_nrw_RPL18B_CP.jpg" width="200" >  </td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/2/23/YMY09_nrw_RPL18B_Nucleus.jpg" >
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<img src="https://static.igem.org/mediawiki/2012/2/23/YMY09_nrw_RPL18B_Nucleus.jpg" width="200" > </td>
</tr>
</tr>
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<tr>
<tr>
<td> pRNR2 </td>
<td> pRNR2 </td>
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<td>row 4</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/5/56/YRC022_nrw_RNR2_ZRC1.jpg">
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<td>row 4</td>
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<img src="https://static.igem.org/mediawiki/2012/5/56/YRC022_nrw_RNR2_ZRC1.jpg" width="200" >  </td>
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<td> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td>
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<td> <a href="https://static.igem.org/mediawiki/2012/4/41/YRC027_nrw_RNR2_Actin.jpg" >
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<td>row 4</td>
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<img src="https://static.igem.org/mediawiki/2012/4/41/YRC027_nrw_RNR2_Actin.jpg" width="200" ></td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/d/db/YRC032_nrw_RNR2_CP.jpg">
 +
<img src="https://static.igem.org/mediawiki/2012/d/db/YRC032_nrw_RNR2_CP.jpg" width="200" >   </td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/c/ce/YRC037_nrw_RNR2_Nucleus.jpg" >
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<img src="https://static.igem.org/mediawiki/2012/c/ce/YRC037_nrw_RNR2_Nucleus.jpg" width="200"> </td>
</tr>
</tr>
 +
<tr>
<tr>
<td> pREV1 </td>
<td> pREV1 </td>
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<td>row 5</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/f/fd/YRC023_nrw_REV1_ZRC1.jpg" >
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<td>row 5</td>
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<img src="https://static.igem.org/mediawiki/2012/f/fd/YRC023_nrw_REV1_ZRC1.jpg" width="200" >  </td>
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<td>row 5</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/a/a6/YRC028_nrw_REV1_Actin.jpg">
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<td> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td>
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<img src="https://static.igem.org/mediawiki/2012/a/a6/YRC028_nrw_REV1_Actin.jpg" width="200" ></td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/5/5f/YRC033_nrw_REV1_CP.jpg" >
 +
<img src="https://static.igem.org/mediawiki/2012/5/5f/YRC033_nrw_REV1_CP.jpg" width="200" > </td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/b/b8/YRC038_nrw_REV1_Nucleus.jpg">
 +
<img src="https://static.igem.org/mediawiki/2012/b/b8/YRC038_nrw_REV1_Nucleus.jpg" width="200" >   </td>
</tr>  
</tr>  
</table>
</table>
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</div>
</div>
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<div class="col12" id = "spacer4"> </div>
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<div class="row">
 +
<div class="col12" id = "spacer4"> </div> </div>
-
 
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<h2> Wide pixel brightness range (0-4095): </h2>
-
<h2> Narrow pixel brightness range (25-100): </h2>
+
-
 
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<div>
<div>
-
<table class="altrowstable" id="alternatecolor" align="center">
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<table class="altrowstable" id="alternatecolor1" align="center">
<tr>
<tr>
<th></th>
<th></th>
<th> Vacuolar Membrane </th>
<th> Vacuolar Membrane </th>
<th> Actin </th>
<th> Actin </th>
-
<th> Cellular Periphery </th>
+
<th> Plasma Membrane </th>
<th> Nucleus </th>
<th> Nucleus </th>
</tr>
</tr>
 +
<tr>
<tr>
<td> pTDH3 </td>
<td> pTDH3 </td>
-
<td> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td>
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<td width="205px"> <a href="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" >
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<td>row 1</td>
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<img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td>
-
<td>row 1</td>
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<td width="205px"> This strain of yeast cells did not grow; the over expression of the protein killed the cells. </td>
-
<td>row 1</td>
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<td width="205px"> <a href="https://static.igem.org/mediawiki/2012/9/97/YRC030_minmax_TDH3_CP.jpg" >
 +
<img src="https://static.igem.org/mediawiki/2012/9/97/YRC030_minmax_TDH3_CP.jpg" width="200" > </td>
 +
<td width="205px"> The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter. </td>
</tr>
</tr>
 +
<tr>
<tr>
<td> pTEF1 </td>
<td> pTEF1 </td>
-
<td>row 2</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/6/6b/YRC021_minmax_TEF1_ZRC1.jpg" >
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<td> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td>
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<img src="https://static.igem.org/mediawiki/2012/6/6b/YRC021_minmax_TEF1_ZRC1.jpg" width="200" > </td>
-
<td>row 2</td>
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<td> This strain of yeast cells did not grow; the over expression of the protein killed the cells. </td>
-
<td>row 2</td>
+
<td> <a href="https://static.igem.org/mediawiki/2012/e/e6/YRC031_minmax_TEF1_CP.jpg" >
 +
<img src="https://static.igem.org/mediawiki/2012/e/e6/YRC031_minmax_TEF1_CP.jpg" width="200" > </td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/d/da/YRC036_minmax_TEF1_Nucleus.jpg" >
 +
<img src="https://static.igem.org/mediawiki/2012/d/da/YRC036_minmax_TEF1_Nucleus.jpg" width="200" > </td>
</tr>
</tr>
 +
<tr>
<tr>
<td> pRPL18B </td>
<td> pRPL18B </td>
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<td>row 3</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/6/62/YVY015_minmax_RPL18B_ZRC1.jpg" >
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<td> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td>
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<img src="https://static.igem.org/mediawiki/2012/6/62/YVY015_minmax_RPL18B_ZRC1.jpg" width="200" >  </td>
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<td>row 3</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/7/73/YTC03_minmax_RPL18B_Actin.jpg" >
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<td>row 3</td>
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<img src="https://static.igem.org/mediawiki/2012/7/73/YTC03_minmax_RPL18B_Actin.jpg" width="200" > </td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/4/42/YAJ12_minmax_RPL18B_CP.jpg" >
 +
<img src="https://static.igem.org/mediawiki/2012/4/42/YAJ12_minmax_RPL18B_CP.jpg" width="200" >  </td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/e/eb/YMY09_minmax_RPL18B_Nucleus.jpg" >
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<img src="https://static.igem.org/mediawiki/2012/e/eb/YMY09_minmax_RPL18B_Nucleus.jpg" width="200">  </td>
</tr>
</tr>
 +
<tr>
<tr>
<td> pRNR2 </td>
<td> pRNR2 </td>
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<td>row 4</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/8/8e/YRC022_minmax_RNR2_ZRC1.jpg">
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<td>row 4</td>
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<img src="https://static.igem.org/mediawiki/2012/8/8e/YRC022_minmax_RNR2_ZRC1.jpg" width="200" >  </td>
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<td> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td>
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<td> <a href="https://static.igem.org/mediawiki/2012/a/a9/YRC027_minmax_RNR2_Actin.jpg">
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<td>row 4</td>
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<img src="https://static.igem.org/mediawiki/2012/a/a9/YRC027_minmax_RNR2_Actin.jpg" width="200" >  </td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/5/59/YRC032_minmax_RNR2_CP.jpg" >
 +
<img src="https://static.igem.org/mediawiki/2012/5/59/YRC032_minmax_RNR2_CP.jpg" width="200" > </td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/6/67/YRC037_minmax_RNR2_Nucleus.jpg" >
 +
<img src="https://static.igem.org/mediawiki/2012/6/67/YRC037_minmax_RNR2_Nucleus.jpg" width="200" >  </td>
</tr>
</tr>
 +
<tr>
<tr>
<td> pREV1 </td>
<td> pREV1 </td>
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<td>row 5</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/5/5e/YRC023_minmax_REV1_ZRC1.jpg" >
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<td>row 5</td>
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<img src="https://static.igem.org/mediawiki/2012/5/5e/YRC023_minmax_REV1_ZRC1.jpg" width="200" >  </td>
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<td>row 5</td>
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<td> <a href="https://static.igem.org/mediawiki/2012/2/2e/YRC028_minmax_REV1_Actin.jpg" >
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<td> <img src="https://static.igem.org/mediawiki/2012/3/37/YRC020_minmax_TDH3_ZRC1.jpg" width="200" > </td>
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<img src="https://static.igem.org/mediawiki/2012/2/2e/YRC028_minmax_REV1_Actin.jpg" width="200" >  </td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/c/c0/YRC033_minmax_REV1_CP.jpg" >
 +
<img src="https://static.igem.org/mediawiki/2012/c/c0/YRC033_minmax_REV1_CP.jpg" width="200" >  </td>
 +
<td> <a href="https://static.igem.org/mediawiki/2012/c/cc/YRC038_minmax_REV1_Nucleus.jpg" >
 +
<img src="https://static.igem.org/mediawiki/2012/c/cc/YRC038_minmax_REV1_Nucleus.jpg" width="200" > </td>
</tr>  
</tr>  
</table>
</table>
</div>
</div>
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</body>

Latest revision as of 18:24, 26 October 2012

header
iGEM Berkeley iGEMBerkeley iGEMBerkeley

Narrow pixel brightness range (25-1000):

Vacuolar Membrane Actin Plasma Membrane Nucleus
pTDH3 This strain of yeast cells did not grow; the over expression of the protein killed the cells. The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter.
pTEF1 This strain of yeast cells did not grow; the over expression of the protein killed the cells.
pRPL18B
pRNR2
pREV1

Wide pixel brightness range (0-4095):

Vacuolar Membrane Actin Plasma Membrane Nucleus
pTDH3 This strain of yeast cells did not grow; the over expression of the protein killed the cells. The RPL18B promoter already made the nucleus very bright, so we did not need a stronger promoter.
pTEF1 This strain of yeast cells did not grow; the over expression of the protein killed the cells.
pRPL18B
pRNR2
pREV1

Retrieved from "http://2012.igem.org/Team:Berkeley/Project/Localization/PromoterLibrary"