Team:LMU-Munich/Germination Stop

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==Germination Stop==
 
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The goal of our project '''BEAD'''zillus is the production of ''Bacillus subtilis'' spores which display novel proteins on their outer coat. These novel proteins could be used in a variety of filtration systems, such as binding viruses out of blood, metals out of drinking water, or plastic molecules out of the marine environment. However, before introducing our filtering-spore “beads” into the environment, we must ensure that we are not simultaneously introducing a GMO capable of reproducing on its own.  
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[[File:GerminationSTOP.png|100px|right|link=]]
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Spores of ''B. subtilis'' are capable of surviving radiation, high heat, freezing, high pressure, and a variety of chemicals. When eventually presented with the right conditions, they can then germinate into vegetative ''B. subtilis'' cells. Thus, simply exposing the spores to harsh conditions does not guarantee that they will never germinate.
 
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Our goal is to remove the germination capability, while keeping the necessary structural functions of the spores intact.
 
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We have chosen genes involved in germination, and will knock them out to yield a ''B. subtilis'' strain which produces spores incapable of germination. This will be checked with germination assays.
 
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Further, we will investigate the possibility of using a redundant toxin system to immediately kill off any spores which somehow germinate.
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== '''Germination'''STOP ==
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<br>
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<p align="justify">The goal of this module was to yield [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads] which are safe (unable to germinate) and consistently functional (maintain their spore shape and structure throughout time). To achieve this, we sought to remove the germination capability of our spores, while keeping their necessary structural functions intact.</p>
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'''Project Navigation'''
 
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{|
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[[File:GerminationSTOP_cycleIIii.jpg|620px|center]]
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|<font color="#FFFFFF">+--+--+--+--+--+--+--</font>
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<html>
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
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<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=70"/>
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</a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction"><font size="1" face="verdana">Bacillus<BR>Intro</font></a>
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|<html>
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[[File:NEXT.png|right|80px|link=Team:LMU-Munich/safetytour]] [[File:BACK.png|left|80px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks">
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<img src="https://static.igem.org/mediawiki/2012/7/78/BacillusBioBrickBox.png" height=70"/>
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks"><font size="1" face="verdana">Bacillus<BR>BioBrickBOX</font></a>
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</html>
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|<html>
 
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins">
 
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<img src="https://static.igem.org/mediawiki/2012/c/c1/SporeCoat.png" height=70"/>
 
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins"><font size="1" face="verdana">SporeCoat<BR>FusionProteins</font></a>
 
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</html>
 
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|<html>
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
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<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=70"/>
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop"><font size="1" face="verdana">Germination<BR>STOP</font></a>
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</html>
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<div class="box">
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==Gene Knockouts==
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{| "width=100%" style="text-align:center;" style="align:right"|
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|<p align="justify">We picked important germination genes and knocked them out. Subsequently we combined the single mutants into quadruple mutants and successfully prevented germination.</p>
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|[[File:LMU Germination STop plate.png|200px|right|link=Team:LMU-Munich/Germination_Stop/Knockout]]
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|-
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Germination_Stop/Knockout]]
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|}
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</div>
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<div class="box">
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=='''Suicide'''switch==
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{| "width=100%" style="text-align:center;" style="align:right"|
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|<p align="justify">In case of germination gene knockout failure, we invented the suicide switch. If spores still germinate, the production of a toxin leads to immediate cell death.</p>
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|[[File:LMU SuicideSwitch grafik.png|200px|right|link=Team:LMU-Munich/Germination_Stop/SuicideSwitch]]
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|-
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Germination_Stop/SuicideSwitch]]
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|}
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</div>
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<br>
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<br>
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<br>
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<br>
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<div class="box">
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====Project Navigation====
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{| width="100%" align="center" style="text-align:center;"
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|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
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|-
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|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
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|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
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|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
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|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
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|}
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Latest revision as of 16:42, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU streaked plate.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

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