Team:LMU-Munich/Germination Stop

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== '''Germination'''STOP ==
 
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== '''Germination'''STOP ==
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<p align="justify">The goal of this module was to yield [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads] which are safe (unable to germinate) and consistently functional (maintain their spore shape and structure throughout time). To achieve this, we sought to remove the germination capability of our spores, while keeping their necessary structural functions intact.</p>  
<p align="justify">The goal of this module was to yield [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads] which are safe (unable to germinate) and consistently functional (maintain their spore shape and structure throughout time). To achieve this, we sought to remove the germination capability of our spores, while keeping their necessary structural functions intact.</p>  
[[File:GerminationSTOP_cycleIIii.jpg|620px|center]]
[[File:GerminationSTOP_cycleIIii.jpg|620px|center]]
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[[File:NEXT.png|right|80px|link=Team:LMU-Munich/safetytour]] [[File:BACK.png|left|80px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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==Gene Knockouts==
==Gene Knockouts==
{| "width=100%" style="text-align:center;" style="align:right"|
{| "width=100%" style="text-align:center;" style="align:right"|
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|<p align="justify">We picked important germination genes and knocked them out. Subsequently we combined the single mutants up to quadruple mutants and prevented successfully germination.</p>  
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|<p align="justify">We picked important germination genes and knocked them out. Subsequently we combined the single mutants into quadruple mutants and successfully prevented germination.</p>  
|[[File:LMU Germination STop plate.png|200px|right|link=Team:LMU-Munich/Germination_Stop/Knockout]]
|[[File:LMU Germination STop plate.png|200px|right|link=Team:LMU-Munich/Germination_Stop/Knockout]]
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=='''Suicide'''switch==
=='''Suicide'''switch==
{| "width=100%" style="text-align:center;" style="align:right"|
{| "width=100%" style="text-align:center;" style="align:right"|
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|<p align="justify">If germination knockout fail, we invented the suicide switch. In case spores still germinate, the production of a toxin leads to immediate cell death.</p>  
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|<p align="justify">In case of germination gene knockout failure, we invented the suicide switch. If spores still germinate, the production of a toxin leads to immediate cell death.</p>  
|[[File:LMU SuicideSwitch grafik.png|200px|right|link=Team:LMU-Munich/Germination_Stop/SuicideSwitch]]
|[[File:LMU SuicideSwitch grafik.png|200px|right|link=Team:LMU-Munich/Germination_Stop/SuicideSwitch]]
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====Project Navigation====
====Project Navigation====

Latest revision as of 16:42, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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