Team:LMU-Munich/Bacillus BioBricks

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[[File:Bacillus BioBrick Box banner.resized WORDS.JPG|620px|link=]]
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==Bacillus BioBricks==
 
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We will create a toolbox of Bacillus BioBricks to contribute to the registry.
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[[Image:BacillusBioBrickBox.png|100px|right|link=]]
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'''We would like to introduce ''Bacillus'' to the world of iGEM!!!'''
 
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[[Image:LMU-Munich_Introduce_Bacillus.jpg|400px|left]]
 
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This <b>B</b>acillus <b>B</b>io<b>B</b>rick <b>B</b>ox contains compatible vectors and useful promoters.
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=='''B<sup>4</sup>''' - 22 core parts for ''Bacillus subtilis''==
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<br>
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<p align="justify">A major goal of our iGEM project is to [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction introduce ''B. subtilis''] as a new chassis for BioBrick-based synthetic biology. For that purpose, we created a toolbox of <i>Bacillus</i> BioBricks to contribute to the registry to make it accessible to many more future iGEM-teams and the entire public microbiology domain! This ''<b>Bacillus'' B</b>io<b>B</b>rick <b>B</b>ox ('''B<sup>4</sup>''') contains the following ''Bacillus'' specific parts:</p>
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Here is a list of all the vectors we cloned and used. Please note that pMAD is not in a BioBrick standard, but it is a useful tool to knock out genes inBacillus subtilis.
 
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For the use of our vectors, please see our [Team:LMU-Munich/Lab_Notebook/Protocols Protocols] page. A general introduction to Bacillus subtilis and its integrative vectors can be found [[Team:LMU-Munich/Bacillus Introduction|here]].
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<div class="box">
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{| width="100%"
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{-
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks/Vectors|Vectors]]'''
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|name
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks/Promoters|Promoters]]'''
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|E. coli res.
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks/Reporters|Reporters]]'''
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|B. subt. res.
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks/Tags|Affinity tags]]
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|insertion locus
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|description
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|derived from
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|reference
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|pSB<sub>Bs</sub>1C-<i>lacZ</i>
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|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
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|Amp
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|[[File:LMU PromoterIconBC.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters]]
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|Cam
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|[[File:LMU Reporter.png|60px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters]]
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|amyE
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|[[File:Proteinaffinitytagbutton.png|50px|link=Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags]]
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|with <i>lacZ</i> reporter gene
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|pAC6
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|?
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|-
|-
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|valign="top"|<font size="2">
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pSB<sub>''Bs''</sub>1C<br>
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pSB<sub>''Bs''</sub>4S<br>
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pSB<sub>''Bs''</sub>2E<br>
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pSB<sub>''Bs''</sub>1C-''lac''Z<br>
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pSB<sub>''Bs''</sub>3C-''lux''ABCDE<br>
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pSB<sub>''Bs''</sub>4S-P<sub>''xyl''</sub><br>
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pSB<sub>''Bs''</sub>0K-P<sub>''spac''</sub><br>
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'''Sporo'''vector</font>
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|valign="top"|<font size="2">
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Anderson<br>
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P<sub>''liaG''</sub><br>
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P<sub>''veg''</sub><br>
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P<sub>''lepA''</sub><br>
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P<sub>''liaI''</sub><br>
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''xylR''-P<sub>''xyl''</sub></font>
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|valign="top"|<font size="2">
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''gfp''<br>
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''mkate2''<br>
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''lacZ''<br>
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''luc+''<br></font>
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|valign="top"|<font size="2">
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Flag<br>His<br>cMyc<br>Strep<br>HA</font>
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|-
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|}
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</div>
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[[File:NEXT.png|right|80px|link=Team:LMU-Munich/Germination_Stop]]  [[File:BACK.png|left|80px|link=Team:LMU-Munich/Data/differentiation_tour]]
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<p align="justify">Since ''Bacillus subtilis'' is not an organism commonly used in iGEM, please check out our [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction Introduction] to learn more about it.</p>
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<div class="box">
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==''Bacillus'' Vectors  [[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]==
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{| "width=100%" style="text-align:center;" style="align:right"|
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|<p align="justify">We have generated a suite of BioBrick-compatible vectors: three empty insertional backbones with different antibiotic resistances and integration loci, two reporter and two expression vectors.</p>
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|[[File:LMU-Munich-PSBBs1C.png|200px|right|link=Team:LMU-Munich/Bacillus BioBricks/Vectors]]
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|-
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Bacillus BioBricks/Vectors]]
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|}
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</div>
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<div class="box">
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==''Bacillus'' Promoters  [[File:LMU PromoterIconBC.png|100px]]==
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{| "width=100%" style="align:right"|
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|
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<p align="justify">To provide a set of promoters of different strength we characterized several promoters in ''Bacillus subtilis''. Both constitutive and inducible promoters are covered.</p>
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|
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[[File:Promoters overview.png|200px|right|link=Team:LMU-Munich/Bacillus BioBricks/Promoters]]
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|-
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Bacillus BioBricks/Promoters]]
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|}
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</div>
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<div class="box">
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==''Bacillus'' Reporters  [[File:LMU Reporter.png|50px]]==
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{| "width=100%" style="align:right"|
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|
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<p align="justify">We designed and codon-optimized a set of reporters that are commonly used in ''B. subtilis''.</p>
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|
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[[File:LMU GFP.jpg|200px|right|link=Team:LMU-Munich/Bacillus BioBricks/Reporters]]
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|-
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Bacillus BioBricks/Reporters]]
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|}
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</div>
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<div class="box">
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==Affinity Tags  [[File:Proteinaffinitytagbutton.png|50px]]==
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{| "width=100%" style="align:right"|
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|
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<p align="justify">We synthesized 5 affinity tags for protein purification. They all are designed in Freiburg standard with an optimized ribosome binding site upstream. We have not yet tested our tags.</p>
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|-
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Bacillus BioBricks/Tags]]
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|}
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</div>
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<br>
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<br>
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<br>
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<br>
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<div class="box">
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====Project Navigation====
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{| width="100%" align="center" style="text-align:center;"
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|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
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|-
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|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
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|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
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|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
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|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
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|}
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Latest revision as of 16:41, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

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