Team:LMU-Munich/Bacillus BioBricks

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Latest revision as of 16:41, 26 October 2012

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

[ more news ]

Bacillus BioBrick Box banner.resized WORDS.JPG

B⁴ - 22 core parts for Bacillus subtilis

A major goal of our iGEM project is to introduce B. subtilis as a new chassis for BioBrick-based synthetic biology. For that purpose, we created a toolbox of Bacillus BioBricks to contribute to the registry to make it accessible to many more future iGEM-teams and the entire public microbiology domain! This Bacillus BioBrick Box (B⁴) contains the following Bacillus specific parts:

Vectors	Promoters	Reporters	Affinity tags

pSB_Bs1C pSB_Bs4S pSB_Bs2E pSB_Bs1C-lacZ pSB_Bs3C-luxABCDE pSB_Bs4S-P_xyl pSB_Bs0K-P_spac Sporovector	Anderson P_liaG P_veg P_lepA P_liaI xylR-P_xyl	gfp mkate2 lacZ luc+	Flag His cMyc Strep HA

Since Bacillus subtilis is not an organism commonly used in iGEM, please check out our Introduction to learn more about it.

Bacillus Vectors


We have generated a suite of BioBrick-compatible vectors: three empty insertional backbones with different antibiotic resistances and integration loci, two reporter and two expression vectors.

Bacillus Promoters


To provide a set of promoters of different strength we characterized several promoters in Bacillus subtilis. Both constitutive and inducible promoters are covered.

Bacillus Reporters


We designed and codon-optimized a set of reporters that are commonly used in B. subtilis.

Affinity Tags


We synthesized 5 affinity tags for protein purification. They all are designed in Freiburg standard with an optimized ribosome binding site upstream. We have not yet tested our tags.

Project Navigation


Bacillus Intro	Bacillus BioBrickBox	Sporobeads	Germination STOP

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks"

@@ Line 1: / Line 1: @@
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-{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_culture_tubes.jpg|3}}
+{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_culture_tubes.resized.jpg|3}}
+[[File:Bacillus BioBrick Box banner.resized WORDS.JPG|620px|link=]]
-==Bacillus BioBricks==
-We will create a toolbox of Bacillus BioBricks to contribute to the registry.
+[[Image:BacillusBioBrickBox.png|100px|right|link=]]
-'''We would like to introduce ''Bacillus'' to the world of iGEM!!!'''
-[[Image:LMU-Munich_Introduce_Bacillus.jpg|400px|left]]
+=='''B<sup>4</sup>''' - 22 core parts for ''Bacillus subtilis''==
+<br>
+<p align="justify">A major goal of our iGEM project is to [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction introduce ''B. subtilis''] as a new chassis for BioBrick-based synthetic biology. For that purpose, we created a toolbox of <i>Bacillus</i> BioBricks to contribute to the registry to make it accessible to many more future iGEM-teams and the entire public microbiology domain! This ''<b>Bacillus'' B</b>io<b>B</b>rick <b>B</b>ox ('''B<sup>4</sup>''') contains the following ''Bacillus'' specific parts:</p>
+<div class="box">
+{| width="100%"
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks/Vectors|Vectors]]'''
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks/Promoters|Promoters]]'''
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks/Reporters|Reporters]]'''
+| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks/Tags|Affinity tags]]
+|-
+|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
+|[[File:LMU PromoterIconBC.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters]]
+|[[File:LMU Reporter.png|60px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters]]
+|[[File:Proteinaffinitytagbutton.png|50px|link=Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags]]
+|-
+|valign="top"|<font size="2">
+pSB<sub>''Bs''</sub>1C<br>
+pSB<sub>''Bs''</sub>4S<br>
+pSB<sub>''Bs''</sub>2E<br>
+pSB<sub>''Bs''</sub>1C-''lac''Z<br>
+pSB<sub>''Bs''</sub>3C-''lux''ABCDE<br>
+pSB<sub>''Bs''</sub>4S-P<sub>''xyl''</sub><br>
+pSB<sub>''Bs''</sub>0K-P<sub>''spac''</sub><br>
+'''Sporo'''vector</font>
+|valign="top"|<font size="2">
+Anderson<br>
+P<sub>''liaG''</sub><br>
+P<sub>''veg''</sub><br>
+P<sub>''lepA''</sub><br>
+P<sub>''liaI''</sub><br>
+''xylR''-P<sub>''xyl''</sub></font>
+|valign="top"|<font size="2">
+''gfp''<br>
+''mkate2''<br>
+''lacZ''<br>
+''luc+''<br></font>
+|valign="top"|<font size="2">
+Flag<br>His<br>cMyc<br>Strep<br>HA</font>
+|-
+|}
+</div>
+[[File:NEXT.png|right|80px|link=Team:LMU-Munich/Germination_Stop]]  [[File:BACK.png|left|80px|link=Team:LMU-Munich/Data/differentiation_tour]]
+<p align="justify">Since ''Bacillus subtilis'' is not an organism commonly used in iGEM, please check out our [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction Introduction] to learn more about it.</p>
+<div class="box">
+==''Bacillus'' Vectors   [[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]==
+{| "width=100%" style="text-align:center;" style="align:right"|
+|<p align="justify">We have generated a suite of BioBrick-compatible vectors: three empty insertional backbones with different antibiotic resistances and integration loci, two reporter and two expression vectors.</p>
+|[[File:LMU-Munich-PSBBs1C.png|200px|right|link=Team:LMU-Munich/Bacillus BioBricks/Vectors]]
+|-
+! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Bacillus BioBricks/Vectors]]
+|}
+</div>
+<div class="box">
+==''Bacillus'' Promoters   [[File:LMU PromoterIconBC.png|100px]]==
+{| "width=100%" style="align:right"|
+|
+<p align="justify">To provide a set of promoters of different strength we characterized several promoters in ''Bacillus subtilis''. Both constitutive and inducible promoters are covered.</p>
+|
+[[File:Promoters overview.png|200px|right|link=Team:LMU-Munich/Bacillus BioBricks/Promoters]]
+|-
+! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Bacillus BioBricks/Promoters]]
+|}
+</div>
+<div class="box">
+==''Bacillus'' Reporters   [[File:LMU Reporter.png|50px]]==
+{| "width=100%" style="align:right"|
+|
+<p align="justify">We designed and codon-optimized a set of reporters that are commonly used in ''B. subtilis''.</p>
+|
+[[File:LMU GFP.jpg|200px|right|link=Team:LMU-Munich/Bacillus BioBricks/Reporters]]
+|-
+! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Bacillus BioBricks/Reporters]]
+|}
+</div>
+<div class="box">
+==Affinity Tags  [[File:Proteinaffinitytagbutton.png|50px]]==
+{| "width=100%" style="align:right"|
+|
+<p align="justify">We synthesized 5 affinity tags for protein purification. They all are designed in Freiburg standard with an optimized ribosome binding site upstream. We have not yet tested our tags.</p>
+|-
+! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Bacillus BioBricks/Tags]]
+|}
+</div>
+<br>
+<br>
+<br>
+<br>
+<div class="box">
+====Project Navigation====
+{| width="100%" align="center" style="text-align:center;"
+|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
+|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
+|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
+|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
+|-
+|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
+|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
+|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
+|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
+|}
+</div>
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