Revision as of 15:25, 26 October 2012

Team Trieste iGEM 2012

Last month

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Suicide System

We tried to complete the construct, cloning the LL 37 construct inside the Holin ones, but unfortunately we didn't obtain positive colonies.

Antibody

The last month we tested the our antibodies. First, we tried to prove that the antibodies fused with LPP-OmpA fragment are actually localized into the outer membrane. To this end, we performed the FACS analisis, but we soon abandoned this approach because it was non suitable for our purpose. Then we performed an immunofloresence assay on the recombinant bacteria. This technique was more successfull. In order to test the functionality of Ab, we did ELISA test.

Cumate-Switch Regulation

CymR
We tried several times to clone the single J23100-CymR-B0015 Eco/Pst into the plasmid K510012 but we didn't have positive colonies.

Contact us

For other information, write to:

igem2012@gmail.com

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 		    	<h2 class="notebook_title">Antibody</h2>
-The hybridoma producing a new neutralizing scFv against norovirus arrived. We extracted its mRNA and recuperated its cDNA. We cloned VL and VH fragments into a sequencing plasmid and now we are waiting to the sequences to arrive. Our hope is to compare two scFv and determine which binds better norovirus VLPs.
+The last month we tested the our antibodies. First, we tried to prove that the antibodies fused with LPP-OmpA fragment are actually localized into the outer membrane. To this end, we performed the FACS analisis, but we soon abandoned this approach because it was non suitable for our purpose. Then we performed an immunofloresence assay on the recombinant bacteria. This technique was more successfull. In order to test the functionality of Ab, we did ELISA test.
 		    </div>
 		    <div id="chassis" class="notebook_section">

Team:Trieste/notebooklast

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