Team:LMU-Munich/Attributions

From 2012.igem.org

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<p align="justify">Here we attribute work done by others and ourselves. We distinguish work done by the team from work done by others, including the Mascher Work Group (our host lab), LMU lab groups, advisors, instructors, graduate students, and postgraduate masters students.</p>
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==Attributions==
 
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On this page, our team attributes work done by others and ourselves. We distinguish work done by the team from work done by others, including the Mascher Work Group (our host lab), LMU lab groups, advisors, instructors, graduate students, and postgraduate masters students.
 
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|<b>Things we have received from the Mascher Work Group:</b>
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|<b>Materials we have received from the Mascher research group:</b>
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| - ''Bacillus subtilis'' WT strain W168
| - ''Bacillus subtilis'' WT strain W168
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| - gDNA from ''B. subtilis'' W168
| - gDNA from ''B. subtilis'' W168
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| - Several plasmids (pDG148, pDG1716, pAC6, pDG1662, [plasmids as templates for resistance cassettes])
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| - gDNA from ''B. lincheniformes''
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| <p align="justify">- Several plasmids Plasmids for  [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks <b>''Bacillus''</b> <b>B</b>io<b>B</b>rick<b>B</b>ox]: pDG148, pDG1716, pAC6, pDG1662, pAH328, pXT, pCSlux103, pAX01, plasmids as templates for resistance cassettes for [https://static.igem.org/mediawiki/2012/7/7a/LMU-Munich_2012_Long-Flanking_Homology_PCR.pdf gene knockouts]: pGEMcat, pDG780, pDG647, pDG1726, pDG1513</p>
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| - Several primers
| - Several primers
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| - All [https://2012.igem.org/Team:LMU-Munich/Lab_Notebook/Protocols main protocols] how to work with ''B. subtilis''
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|<b>Services we have received from Dr. Andreas Brachmann:</b>
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| - Sequencing service
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|<b>Work we have received from others:</b>
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|<b>Support we have received from others:</b>
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| - Introduction to fluorescence microscopy by the lab of [http://www.bacteriology.bio.lmu.de/ Marc Brahmkamp]
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| <p align="justify">- Introduction to fluorescence microscopy by the lab of [http://www.bacteriology.bio.lmu.de/ Marc Bramkamp] and some microscopy protocols. All pictures were taken by team members.</p>
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| - [http://jb.asm.org/content/193/16/4075 Imamura ''et al.'']: We received plasmids from his work with the spore crust proteins that we can use as a positive control. The plasmids include ''cotZ-gfp'' and ''cgeA-rfp'' fusion proteins and a ''gfp'', that is produced inside the spore.
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| <p align="justify">- [http://jb.asm.org/content/193/16/4075 Imamura ''et al.'']: We received plasmids from his work with the spore crust proteins that we can use as a positive control. The plasmids include ''cotZ-gfp'' and ''cgeA-rfp'' fusion proteins and a ''gfp'', that is produced inside the spore.</p>
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| - Mathias Plichta helped us with the technical implementation of the website
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| - We used the experience and some materials from the last years LMU-Munich iGEM team for the highschool practical course.
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| <p align="justify">- We used the experience and some materials from last year's LMU-Munich iGEM team for our highschool practical course.</p>
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| -  
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| - Sequencing from [http://www.gi.bio.lmu.de/sequencing Dr. Andreas Brachmann]
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|<b>Work we have done ourselves:</b>
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| - Promoter evaluations: Korinna Kraft
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| <p align="justify">- We used plasmids from the [http://www.bgsc.org/ Bacillus Genetic Stock Center] as template for our [[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|new backbone vectors]].</p>
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| - Construction of our new BioBricks
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| <p align="justify">- gDNA from ''E. coli'' K12 from [http://www.mikrobiologie.biologie.uni-muenchen.de/forschung/ag_k_jung/index.html AG K. Jung]</p>
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| - pRouf<sub>CGU</sub>-lacZ and pURyhB from [http://www.mfpl.ac.at/mfpl-group/group/blaesi.html AG Udo Bläsi]
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'''Project Navigation'''
 
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====Project Navigation====
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{| width="100%" align="center" style="text-align:center;"
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|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
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|[[Team:LMU-Munich/Bacillus_Introduction|<font size="2">'''''Bacillus'''''<BR>Intro</font>]]
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|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="2" face="verdana">'''''Bacillus'''''<BR>'''B'''io'''B'''rick'''B'''ox</font>]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction">
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|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="2" face="verdana">'''Sporo'''beads</font>]]
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<img src="https://static.igem.org/mediawiki/2012/8/8d/Bacilluss_Intro.png" height=70"/>
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|[[Team:LMU-Munich/Germination_Stop|<font size="2" face="verdana">'''Germination'''<BR>STOP</font>]]
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<a href="https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks"><font size="1" face="verdana">Bacillus<BR>BioBrickBOX</font></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins"><font size="1" face="verdana">SporeCoat<BR>FusionProteins</font></a>
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop">
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<a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop"><font size="1" face="verdana">Germination<BR>STOP</font></a>
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{{:Team:LMU-Munich/Templates/Page Footer}}
{{:Team:LMU-Munich/Templates/Page Footer}}

Latest revision as of 13:50, 26 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU sarstedt solutions.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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