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Team:Trieste/parts
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<tr> | <tr> | ||
| - | <th>Name</th> | + | <th> Name</th> |
| - | <th>Seq 5'--> 3'</th> | + | <th> Seq 5'--> 3'</th> |
| - | <th>Notes</th> | + | <th> PCR Notes</th> |
</tr> | </tr> | ||
</thead> | </thead> | ||
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<tr><td>T5EcoFw</td> <td>CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT</td> <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | <tr><td>T5EcoFw</td> <td>CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT</td> <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
<tr><td>T5XhoRev</td> <td>TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA</td> <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | <tr><td>T5XhoRev</td> <td>TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA</td> <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta1/2FW</td> <td>ATACTACGACGGTAAATGGT</td> <td>95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta1/2REV</td> <td>TACATCAGTATCGGTAGCAT</td> <td>95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta7/8FW</td> <td>GACCAAGCGATAACCGGATG</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta7/8REV</td> <td>GTGAGATGATGGCCACGATT</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta9/10FW</td> <td>GCGAGGTAACCTCGAACATG</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
| + | <tr><td>Muta9/10REV</td> <td>CGGCGTATCGATAATTCACG</td> <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
Latest revision as of 12:03, 26 October 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Parts Overview
More
List of the Submitted Parts
Click on the code of each part to have more information about the Biobrick, its construction, the results obtained and the relative link to the Registry.
| Name | Type | Description | ||
| BBa_K875001 | Regulatory | T5 Cumate Operator | ||
| BBa_K875002 | Regulatory | T5 Lac Operator | ||
| BBa_K875003 | Composit | CymR | ||
| BBa_K875004 | Composit | T5LacO-LPP-OmpA-scFv | ||
| BBa_K875005 | Composit | T5LacO-LPP-OmpA-SIP | ||
| BBa_K875006 | Composit | T5LacO-LPP-PelB-scFv | ||
| BBa_K875007 | Composit | T5 LacO-LPP-PelB-SIP | ||
| BBa_K875008 | Coding | IPTG inducible Tse2 toxin | ||
| BBa_K875009 | Coding | LL 37 - Cathelicidin | ||
| BBa_K875020 | Composit | β-Glucosidase |
Primers
| Name | Seq 5'--> 3' | PCR Notes |
|---|---|---|
| Vr | ATTACCGCCTTTGAGTGAGC | 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞ |
| Vf2 | TGCCACCTGACGTCTAAGAA | 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞ |
| GlmSTn7Fw | GCATGTGGAAGAGGTGATTG | 95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| GlmSTn7Rev | ACTTTATTGTTCGCCCACA | 95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| B0015XhoRev | ACTCGAGATTACTAGTTATAAACGCAGAAAGGCCCACCC | 95° 5' | 30x (93° 30'' | 58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| B0015EcoFw | CGAATTCATCAGATCTCCAGGCATCAAATAAAACGAAAGG | 95° 5' | 30x (93° 30'' |58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| T5EcoFw | CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT | 95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| T5XhoRev | TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA | 95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta1/2FW | ATACTACGACGGTAAATGGT | 95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta1/2REV | TACATCAGTATCGGTAGCAT | 95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta7/8FW | GACCAAGCGATAACCGGATG | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta7/8REV | GTGAGATGATGGCCACGATT | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta9/10FW | GCGAGGTAACCTCGAACATG | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
| Muta9/10REV | CGGCGTATCGATAATTCACG | 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞ |
