Team:Trieste/parts

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<h2>List of the Submitted Parts </h2>
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<groupparts>"iGEM12 Trieste</groupparts>
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<h3>Click on the code of each part to have more information about the Biobrick, its construction, the results obtained and the relative link to the Registry.</h3>
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</br>
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<center>
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<table>
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    <tbody>
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<tr>
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    <td><strong>Name</strong></td>
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                                    <td></td>
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    <td><strong>Type</strong></td>
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                                    <td></td>
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    <td><strong>Description</strong></td>
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                                </tr>
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<tr>
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    <td><a href="https://2012.igem.org/Team:Trieste/parts/1">BBa_K875001</a></td>  
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                                    <td></td>
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    <td>Regulatory</td>
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                                    <td></td>
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    <td>T5 Cumate Operator</td>
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                                </tr>
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<tr>
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    <td><a href="https://2012.igem.org/Team:Trieste/parts/2">BBa_K875002</a></td>
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                                    <td></td>
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    <td>Regulatory</td>
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                                    <td></td>
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    <td>T5 Lac Operator</td>
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                                </tr>
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<tr>
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    <td><a href="https://2012.igem.org/Team:Trieste/parts/3">BBa_K875003</a></td>
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                                    <td></td>
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    <td>Composit</td>
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                                    <td></td>
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    <td>CymR</td>
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                                </tr>
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<tr>
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    <td><a href="https://2012.igem.org/Team:Trieste/parts/4">BBa_K875004</a></td>
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                                    <td></td>
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    <td>Composit</td>
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                                    <td></td>
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    <td>T5LacO-LPP-OmpA-scFv</td>
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                                </tr>
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<tr>
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    <td><a href="https://2012.igem.org/Team:Trieste/parts/5">BBa_K875005</a></td>
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                                    <td></td>
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    <td>Composit</td>
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                                    <td></td>
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    <td>T5LacO-LPP-OmpA-SIP</td>
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                                </tr>
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<tr>
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    <td><a href="https://2012.igem.org/Team:Trieste/parts/6">BBa_K875006</a></td>
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                                    <td></td>
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    <td>Composit</td>
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                                    <td></td>
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    <td>T5LacO-LPP-PelB-scFv</td>
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                                </tr>
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<tr>
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    <td><a href="https://2012.igem.org/Team:Trieste/parts/7">BBa_K875007</a></td>
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                                    <td></td>
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    <td>Composit</td>
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                                    <td></td>
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    <td>T5 LacO-LPP-PelB-SIP</td>
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                                </tr>
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<tr>
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    <td><a href="https://2012.igem.org/Team:Trieste/parts/8">BBa_K875008</a></td>
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                                    <td></td>
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    <td>Coding</td>
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                                    <td></td>
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    <td>IPTG inducible Tse2 toxin</td>
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                                </tr>
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<tr>
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    <td><a href="https://2012.igem.org/Team:Trieste/parts/9">BBa_K875009</a></td>
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                                    <td></td>
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    <td>Coding</td>
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                                    <td></td>
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    <td>LL 37 - Cathelicidin</td>
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                                </tr>
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<tr>
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    <td><a href="https://2012.igem.org/Team:Trieste/parts/10">BBa_K875020</a></td>
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                                    <td></td>
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    <td>Composit</td>
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                                    <td></td>
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    <td>β-Glucosidase</td>
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                                </tr>
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                            </tbody>
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                        </table>   
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</center>
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<br/>
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<h2>Primers </h2>
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<table>
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<thead>
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<tr>
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<th>  Name</th>
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<th>  Seq 5'--> 3'</th>
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<th>  PCR Notes</th>
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</tr>
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</thead>
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<tbody>
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<tr><td>Vr</td>              <td>ATTACCGCCTTTGAGTGAGC</td>      <td>95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞</td></tr>
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<tr><td>Vf2</td>              <td>TGCCACCTGACGTCTAAGAA</td>    <td> 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞</td></tr>
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<tr><td>GlmSTn7Fw</td>        <td>GCATGTGGAAGAGGTGATTG</td>      <td>95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr>
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<tr><td>GlmSTn7Rev</td>      <td>ACTTTATTGTTCGCCCACA</td>            <td>95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr>
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<tr><td>B0015XhoRev</td>      <td>ACTCGAGATTACTAGTTATAAACGCAGAAAGGCCCACCC</td>    <td>95° 5' | 30x (93° 30'' | 58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr>
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<tr><td>B0015EcoFw</td>      <td>CGAATTCATCAGATCTCCAGGCATCAAATAAAACGAAAGG</td>  <td>95° 5' | 30x (93° 30'' |58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td> </tr>
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<tr><td>T5EcoFw</td>          <td>CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT</td>  <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr>
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<tr><td>T5XhoRev</td>        <td>TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA</td>  <td>95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr>
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              <tr><td>Muta1/2FW</td>        <td>ATACTACGACGGTAAATGGT</td>  <td>95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr>
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              <tr><td>Muta1/2REV</td>        <td>TACATCAGTATCGGTAGCAT</td>  <td>95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr>
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              <tr><td>Muta7/8FW</td>        <td>GACCAAGCGATAACCGGATG</td>  <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr>
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              <tr><td>Muta7/8REV</td>        <td>GTGAGATGATGGCCACGATT</td>  <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr>
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              <tr><td>Muta9/10FW</td>      <td>GCGAGGTAACCTCGAACATG</td>  <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr>
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              <tr><td>Muta9/10REV</td>      <td>CGGCGTATCGATAATTCACG</td>  <td>95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞</td></tr>
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</tbody>
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</table>
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    <!--  ******************** CONTENUTO QUI ****************** -->  
    <!--  ******************** CONTENUTO QUI ****************** -->  
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             <ul id="sub_menu">
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             <ul id="sub_menu"><strong>
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                 <li><a href="#">Sotto menu 1</a></li>
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                 <li class="select"><a href="https://2012.igem.org/Team:Trieste/parts">Overwiev</a></li>
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                    <li class="select"><a href="#">Sotto menu 2</a></li>
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                <li><a href="https://2012.igem.org/Team:Trieste/parts/1">BBa_K875001 - Cumate Op</a></li>
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                    <li><a href="#">Sotto menu 3</a></li>
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                <li><a href="https://2012.igem.org/Team:Trieste/parts/2">BBa_K875002 - Lac OP</a></li>
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                    <li><a href="#">Sotto menu 4</a></li>
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                <li><a href="https://2012.igem.org/Team:Trieste/parts/3">BBa_K875003 - CymR</a></li>
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                    <li><a href="#">Sotto menu 5</a></li>
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                <li><a href="https://2012.igem.org/Team:Trieste/parts/4">BBa_K875004 - OmpA scFv</a></li>
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                <li><a href="https://2012.igem.org/Team:Trieste/parts/5">BBa_K875005 - OmpA SIP</a></li>
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                <li><a href="https://2012.igem.org/Team:Trieste/parts/6">BBa_K875006 - PelB scFv</a></li>
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                <li><a href="https://2012.igem.org/Team:Trieste/parts/7">BBa_K875007 - PelB SIP </a></li>
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                <li><a href="https://2012.igem.org/Team:Trieste/parts/8">BBa_K875008 - Tse2 Toxin</a></li>
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                <li><a href="https://2012.igem.org/Team:Trieste/parts/9">BBa_K875009 - LL 37</a></li>
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                <li><a href="https://2012.igem.org/Team:Trieste/parts/10">BBa_K875020 - Glucosidase</a></li>
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</strong>
                 </ul>
                 </ul>
                 <img src="https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg" alt="Team iGEM 2012" id="igem_team" />
                 <img src="https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg" alt="Team iGEM 2012" id="igem_team" />
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Latest revision as of 12:03, 26 October 2012

Parts Overview

More

List of the Submitted Parts

Click on the code of each part to have more information about the Biobrick, its construction, the results obtained and the relative link to the Registry.



Name Type Description
BBa_K875001 Regulatory T5 Cumate Operator
BBa_K875002 Regulatory T5 Lac Operator
BBa_K875003 Composit CymR
BBa_K875004 Composit T5LacO-LPP-OmpA-scFv
BBa_K875005 Composit T5LacO-LPP-OmpA-SIP
BBa_K875006 Composit T5LacO-LPP-PelB-scFv
BBa_K875007 Composit T5 LacO-LPP-PelB-SIP
BBa_K875008 Coding IPTG inducible Tse2 toxin
BBa_K875009 Coding LL 37 - Cathelicidin
BBa_K875020 Composit β-Glucosidase

Primers

Name Seq 5'--> 3' PCR Notes
Vr ATTACCGCCTTTGAGTGAGC 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞
Vf2 TGCCACCTGACGTCTAAGAA 95° 5' | 30x ( 93° 30" | 50° 30" | 72° 30-120") | 72° 7' | 4° ∞
GlmSTn7Fw GCATGTGGAAGAGGTGATTG 95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
GlmSTn7Rev ACTTTATTGTTCGCCCACA 95° 5' | 30x (93° 30'' | 53.8° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
B0015XhoRev ACTCGAGATTACTAGTTATAAACGCAGAAAGGCCCACCC 95° 5' | 30x (93° 30'' | 58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
B0015EcoFw CGAATTCATCAGATCTCCAGGCATCAAATAAAACGAAAGG 95° 5' | 30x (93° 30'' |58° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
T5EcoFw CGAATTCATGAGATCTAAATCATAAAAAATTTATTTGCT 95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
T5XhoRev TCTCGAGTTAGGATCCTTTCTCCTCTTTCTCTAGTAATA 95° 5' | 30x (93° 30'' | 52° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
Muta1/2FW ATACTACGACGGTAAATGGT 95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
Muta1/2REV TACATCAGTATCGGTAGCAT 95° 5' | 30x (93° 30'' | 45° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
Muta7/8FW GACCAAGCGATAACCGGATG 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
Muta7/8REV GTGAGATGATGGCCACGATT 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
Muta9/10FW GCGAGGTAACCTCGAACATG 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
Muta9/10REV CGGCGTATCGATAATTCACG 95° 5' | 30x (93° 30'' | 50° 30'' | 72° 30-120'' ) | 72° 7' | 4° ∞
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