From 2012.igem.org
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| - | {{:Team:Cornell/Navbar}}
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| - | {{:Team:Cornell/TOC|right}}
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| - | =June=
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| - | ==June 10th-16th==
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| - | ===June 13th, Wednesday===
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| - | *Set up [[Team:Cornell/Notebook/Phusion_PCR|PCR]] for four fragments of the nah operon as well as the pBMT-1 backbone, using primers designed to mutate cut-sites concurrently with Gibson Assembly. Also set up a PCR for the entire nah operon. Due to length of the fragments, a longer extension time was chosen.
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| - | *If Gibson Assembly fails, but we are able to PCR the entire 10kb nah operon, an alternate method of mutation using three sequential site-directed mutageneses will be pursued.
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| - | **''Work done by: Caleb, Claire, Dylan, Spencer, Steven, and Swati''
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| - | ===June 14th, Thursday===
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| - | *PCR only amplified nah1, nah3, and nah4 - longer fragments (nah2, the full nah operon, and pBMT-1) were not identified on the PCR product gel. Set up another [[Team:Cornell/Notebook/Phusion_PCR|PCR]] with shorter extension time.
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| - | ===June 15th, Friday===
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| - | *PCR of four out of five products visible on gel. Set up PCR of pBMT-1, final fragment required for Gibson Assembly of the nah operon.
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| - | ==June 17th-23rd==
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| - | ==June 24th-30th==
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| - | ===June 28th, Wednesday===
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| - | [[Image:2012_6_28_StevenArchieGelLoading.jpg|thumb|right|Stephen and Archie Gel purifying our arsR construct]]
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| - | *Gel purified arsR construct
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| - | =July=
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| - | =August=
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Latest revision as of 06:40, 26 October 2012
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