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      <p align="justify">With the valuable collaboration of the team  iGEM-Alberta 2008, we link the the light-oxygen dependent regulation systems  with the butanol production pathway and its electron-transfer-flavoprotein  alpha and betha polypeptides, this constructions were sent by Dr. Douglas  Ridgway. (Reference: <a href="https://2008.igem.org/Team:The_University_of_Alberta/Butanerd" target="_blank">Butanerd</a>)</p>
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      <p align="justify"><em>Clostridium</em><em> acetobutylicum</em> is an anaerobic,  spore-forming bacterium with the ability to ferment starch and sugars into  solvents. In the past, it has been used for industrial production  of acetone and butanol, until cheap crude oil  rendered petrochemical synthesis more economically feasible. Both economic and  environmental aspects have caused the pendulum to swing back again. Molecular  biology has allowed a detailed understanding of genes and enzymes, required for  solventogenesis.(1)<br />
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        Taking advantage of the metabolic versatility of <em>R. sphearoides</em> and <em>R. palustris</em> and their ability to fix Carbon dioxide, we aim to  introduce the following constructions into these both bacteria so that the  fixed carbon dioxide becomes into butanol.</p>
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      <p align="justify">We already constructed  the following circuits, ready for testing in <em>R. Palustris</em></p>
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      <p>&nbsp;</p>
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      <p align="justify">We have to link these  constructions into the vector pRK415, and conjugate <em>R. sphearoides</em> and <em>R. palutris</em>, to finally test our system and to produce butanol.</p>
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      <h1>References</h1>
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      <p><strong>&nbsp;</strong></p>
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      <h1 align="justify">1.- <strong>Fermentative butanol  production: bulk chemical and biofuel</strong>. Ann N Y Acad Sci., Dürre  P. 2008 Mar;1125:353-62.</h1>
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      <h1>2.-Cong T. Trinh,<strong> Elucidating and reprogramming  Escherichia coli metabolisms for obligate anaerobic n-butanol and isobutanol  production</strong> Appl Microbiol Biotechnol DOI  10.1007/s00253-012-4197-7</h1>
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      <p align="justify">3.-Masayuki Inui &amp; Masako Suda &amp; Sakurako Kimura &amp; Kaori  Yasuda &amp; Hiroaki Suzuki &amp; Hiroshi Toda &amp; Shogo Yamamoto &amp;  Shohei Okino &amp; Nobuaki Suzuki &amp; Hideaki Yukawa<strong> Expression  of <em>Clostridium acetobutylicum</em> butanol synthetic genes in <em>Escherichia coli</em> </strong>Appl Microbiol  Biotechnol (2008) 77:1305–1316</p>
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        4.-Wolfram Andersch, Hubert Bahl, and Gerhard Gottschalk Eur J <strong>Level  of Enzymes Involved in Acetate, Butyrate, Acetone and Butanol Formation by <em>Clostridium  acetobutylicum </em></strong>Appl Microbiol Biotechnol (1983) 18:327-332</p>
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        5.-Hubert Bahl, Wolfram Andersch, and  Gerhard Gottschalk European J <strong>Continuous  Production of Acetone and Butanol by <em>Clostridium acetobutylicum </em>in a  Two-Stage Phosphate Limited Chemostat</strong> Appl Microbiol Biotechnol (1982) 15:201-205 </p>
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