Team:LMU-Munich/Application

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=Application=
 
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<p align="justify">There are many possible applications for our [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads] as it is possible to modify their outermost protein layer. This way they could be used as beads with special functions. To easily create any kind of '''Sporo'''bead we designed a [https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks/Sporovector '''Sporo'''vector], where you just have to insert your gene of choice. Since there are so many possible applications we picked three examplary ideas for future '''Sporo'''beads: </p>
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What are the potential '''advantages''' of using ''B. subtilis'' spores as functional bioparticals?
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<br>'''Kumamolisin'''-'''Sporo'''beads: the solution for carefree enjoyment of everyday meals
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World wide one out of [http://www.enriquecastro.net/index.php/term/,9da4ab975b545ba0ae53646c58a5a265aa5d535892a89b979fa4b1a49297a261a260555c5a.xhtml 3350] people cannot eat meals that contain wheat products and other foods with traces of gluten. Kumamolisin is an [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590087 enzyme] that cleaves peptides and was produced by the iGEM-Team from the University of [https://2011.igem.org/Team:Washington Washington]] last year. The substrate includes a specific sequence of amino acids which causes celiac disease in sensitive people when they consume food containing gluten. Our beads could carry Kumamolisin and offer a protected passage through the stomach, so that the enzyme can work properly where it is needed in the intestines. The [https://2012.igem.org/Team:LMU-Munich/Germination_Stop <b>Germination</b>STOP] we put in place in our spores would ensure a correct dosage. This project is a pharmaceutical application and therefore would have to fulfill the laws for pharmaceuticals. This includes several verification steps of non-toxicity and efficacy.
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'''CPX'''-'''Sporo'''beads: rescuing marine life
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The excessive use of disposable plastic and the lack of universal recycling programs has led to the pollution of the world's oceans. In the ocean, large pieces of Polystyrene litter are ground by sea currents into very small pieces, so called plastik plankton, that are consumed by fish, filter feeders, and other organisms living in the oceans. Such plastic uptake can lead to poisoning, sterility and death. The [http://partsregistry.org/wiki/index.php/Part:BBa_I728500 CPX-peptide] generated by MIT (2007) can bind to Polystyrene. CPX-'''Sporo'''beads in huge filter boxes could be put into place to mechanically filter microscopic plastic particles, like polystyrene plankton, out of the water. To prevent the beads from being released into the sea and the plastic to be kept the '''Sporo'''beads could be attached to membranes in the boxes. The spores would then not only express CPX but also a membrane binding protein on their surface.
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'''TALE'''-'''Sporo'''beads: easy and cheap detection of GMOs
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<br>They are:
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Since 1990, green biotechnology releases many transgenic plants into the environment by selling genetically modified seeds. Thus organic farmers need to prove today, that their products meet the requirements for organic crops. Usually they pay laboratories to attest the lack of contamination with genetically modified crops. The new tools of molecular biology, TAL effectors, combined with our Sporobeads could be an easy and cheap solution for organic agriculture. Farmers could in future use our kit with TALE-''lacZ''-'''Sporo'''beads to detect genetically modified crops themselves. As spores are stable and safe vehicles, they could be send by mail without any considerations. The kit would be suitable for use outside of laboraty. The protocol for this could work as follows:
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<br>The DNA extracted from plants with solutions provided by the kit, is immobilized and fixed on a nitrocellulose membrane. This membrane is then washed, incubated with '''Sporo'''beads in solution and washed again. With addition of the substrate X-Gal, the ''lacZ'' of bound '''Sporo'''beads will catalyze the reaction so that a blue staining appears. If no such DNA is present, the spores will not bind and no blue color will appear.</p>
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|[[File:BoxChecked.png|40px|left]]
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|Very stable even under severe environmental conditions
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In general, ''B. subtilis'' spores are suitable for high-scale applications: they are
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*easy and cheap to produce in large quantities ([http://www.alibaba.com/product-free/118791994/Bacillus_subtilis_spores_HU58_100_pure.html offer])
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|[[File:BoxChecked.png|40px|left]]
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*very stable in several extreme environment conditions
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|Easy and cheap to produce in large quantities (check out the link to this commercial [http://www.alibaba.com/product-free/118791994/Bacillus_subtilis_spores_HU58_100_pure.html offer])
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*[https://2012.igem.org/Team:LMU-Munich/Project_Safety unable] to proliferate and therefore most likely not treated as genetically modified organisms by law
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*part of human nutrition in [http://en.wikipedia.org/wiki/Bacillus_subtilis_R0179 food supplements] and generally regarded as safe
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|[[File:BoxChecked.png|40px|left]]
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|Part of human nutrition in terms of [http://en.wikipedia.org/wiki/Bacillus_subtilis_R0179 food supplements] and  [http://www.fda.gov/Food/FoodIngredientsPackaging/GenerallyRecognizedasSafeGRAS/default.htm generally regarded as safe]
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|colspan="2"|Moreover, our '''Sporo'''beads are:
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|[https://2012.igem.org/Team:LMU-Munich/Project_Safety Unable] to proliferate and therefore most likely not treated as genetically modified organisms by the law
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<p align="justify">Our [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads] offer a wide variety of applications within the laboratory, and around the world. '''Sporo'''beads can be used for filtration and protein screening.</p>
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====Filtration====
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{| width="100%" style="text-align:center;"|
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|<p align="justify">Further information for the first possible application for our '''Sporo'''beads.</p>
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|[[File:LMU Sporofilter.png|right|150px|link=Team:LMU-Munich/Application/Filtration]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Application/Filtration]]
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====Protein Screening====
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{| width="100%" style="text-align:center;"|
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|<p align="justify">Further information for the possible application of protein screening.</p>
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|[[File:protein_libraries.jpg|right|150px|link=Team:LMU-Munich/Application/Protein Screening]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Application/Protein Screening]]
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====Further Applications====
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{| width="100%" style="text-align:center;"|
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|<p align="justify">More information for further possible application with our diverse '''Sporo'''beads.</p>
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|[[File:LMU Sporo diversity.png|right|150px|link=Team:LMU-Munich/Application/Further Applications]]
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Application/Further Applications]]
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====Project Navigation====
====Project Navigation====

Latest revision as of 23:24, 25 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo2.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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