Team:LMU-Munich/Data/crustpromoters

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<p align="justify">The figure below shows the result of the evaluation of the three promoters (P<sub>''cgeA''</sub>, P<sub>''cotV''</sub>, P<sub>''cotYZ''</sub>) for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 BBa_K823025]. For each promoter two independent clones were evaluated three times. To link the promoter activities to the growth phase, the optical density (OD<sub>600</sub>) was measured throughout the whole experiment. </p>
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<p align="justify">The figure below shows the result of the evaluation of the three promoters (P<sub>''cgeA''</sub>, P<sub>''cotV''</sub>, P<sub>''cotYZ''</sub>) for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 pSB<sub>''Bs''</sub>3C-<i>lux</i>ABCDE]. For each promoter two independent clones were evaluated three times. To link the promoter activities to the growth phase, the optical density (OD<sub>600</sub>) was measured throughout the whole experiment. </p>
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<font color="#000000"; size="2"><p align="justify">Fig. 1:The result of the measurement of the three promoters P<sub>''cgeA''</sub>, P<sub>''cotV''</sub> and P<sub>''cotYZ''</sub> fused to the lux operon. The left vertical axis displays the luminescence divided by the OD<sub>600</sub> and the left one shows the OD<sub>600</sub>. On the horizontal axis the time is illustrated. The continuous lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.</p></font>
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<font color="#000000"; size="2"><p align="justify">Fig. 1: The result of the measurement of the three promoters P<sub>''cgeA''</sub>, P<sub>''cotV''</sub> and P<sub>''cotYZ''</sub> fused to the lux operon. The left vertical axis displays the luminescence divided by the OD<sub>600</sub> and the left one shows the OD<sub>600</sub>. On the horizontal axis the time is illustrated. The continuous lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.</p></font>
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<p align="justify">The native promoters of ''cotZ'', P<sub>''cotYZ''</sub> and  P<sub>''cotV''</sub>, show a clear signal late in stationary phase, as expected. P<sub>''cotYZ''</sub> is activated two hours earlier than P<sub>''cotV''</sub> and both promoters are active for about 12 hours. In contrast, P<sub>''cgeA''</sub> does not show any activity. </p>
<p align="justify">The native promoters of ''cotZ'', P<sub>''cotYZ''</sub> and  P<sub>''cotV''</sub>, show a clear signal late in stationary phase, as expected. P<sub>''cotYZ''</sub> is activated two hours earlier than P<sub>''cotV''</sub> and both promoters are active for about 12 hours. In contrast, P<sub>''cgeA''</sub> does not show any activity. </p>
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Latest revision as of 14:06, 24 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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