Team:LMU-Munich/Data/crustpromoters

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Latest revision as of 14:06, 24 October 2012

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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Crust promoter evaluation

The figure below shows the result of the evaluation of the three promoters (P_cgeA, P_cotV, P_cotYZ) for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 pSB_Bs3C-luxABCDE]. For each promoter two independent clones were evaluated three times. To link the promoter activities to the growth phase, the optical density (OD₆₀₀) was measured throughout the whole experiment.

Protein distribution in spore coat of Bacillus subtilis

Fig. 1: The result of the measurement of the three promoters P_cgeA, P_cotV and P_cotYZ fused to the lux operon. The left vertical axis displays the luminescence divided by the OD₆₀₀ and the left one shows the OD₆₀₀. On the horizontal axis the time is illustrated. The continuous lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.

The native promoters of cotZ, P_cotYZ and P_cotV, show a clear signal late in stationary phase, as expected. P_cotYZ is activated two hours earlier than P_cotV and both promoters are active for about 12 hours. In contrast, P_cgeA does not show any activity.

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Data/crustpromoters"

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-====Crust promoter measurements====
-<p align="justify">The figure below shows the result of the evaluation of the three promoters for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 BBa_K823025]. Each promoter (P<sub>''cgeA''</sub>, P<sub>''cotV''</sub>, P<sub>''cotYZ''</sub>) has two independent clones and both were measured three times. To be able to know in which growth phase the cells are the optical density (OD<sub>600</sub>) was measured throughout the whole experiment. </p>
+====Crust promoter evaluation====
+<p align="justify">The figure below shows the result of the evaluation of the three promoters (P<sub>''cgeA''</sub>, P<sub>''cotV''</sub>, P<sub>''cotYZ''</sub>) for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 pSB<sub>''Bs''</sub>3C-<i>lux</i>ABCDE]. For each promoter two independent clones were evaluated three times. To link the promoter activities to the growth phase, the optical density (OD<sub>600</sub>) was measured throughout the whole experiment. </p>
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 {| style="color:black;" cellpadding="0" width="90%" cellspacing="0" border="0" align="center" style="text-align:left;"
 |style="width: 70%;background-color: #EBFCE4;" |
-<font color="#000000"; size="2"><p align="justify">The result of the measurement of the three promoters P<sub>''cgeA''</sub>, P<sub>''cotV''</sub> and P<sub>''cotYZ''</sub>. The left vertical axis displays the luminescence divided by the OD<sub>600</sub> and the left one shows the OD<sub>600</sub>. On the horizontal axis the time is illustrated. The drawn through lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.</p></font>
+<font color="#000000"; size="2"><p align="justify">Fig. 1: The result of the measurement of the three promoters P<sub>''cgeA''</sub>, P<sub>''cotV''</sub> and P<sub>''cotYZ''</sub> fused to the lux operon. The left vertical axis displays the luminescence divided by the OD<sub>600</sub> and the left one shows the OD<sub>600</sub>. On the horizontal axis the time is illustrated. The continuous lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.</p></font>
 |}
 |}
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-<p align="justify">The native promoter of ''cotZ'' P<sub>''cotYZ''</sub> and  P<sub>''cotV''</sub> show a signal in stationary phase whereas P<sub>''cotYZ''</sub> is activated two hours earlier than P<sub>''cotV''</sub>. It is displayed that these promoters are active for about 12 hours while  P<sub>''cgeA''</sub> does not show any acitvity. </p>
+<p align="justify">The native promoters of ''cotZ'', P<sub>''cotYZ''</sub> and  P<sub>''cotV''</sub>, show a clear signal late in stationary phase, as expected. P<sub>''cotYZ''</sub> is activated two hours earlier than P<sub>''cotV''</sub> and both promoters are active for about 12 hours. In contrast, P<sub>''cgeA''</sub> does not show any activity. </p>
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-[[File:Arrow purple Data.png|100px|link=Team:LMU-Munich/Data]]
+[[File:Arrow purple Data.png|center|100px|link=Team:LMU-Munich/Data]]
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