Team:Tianjin/Data

From 2012.igem.org

(Difference between revisions)
 
(15 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Tianjin/frame/data}}
{{:Team:Tianjin/frame/data}}
 +
__NOTOC__
 +
<html>
 +
<style type="text/css">
 +
#secondpane {color:#fff;
 +
width: 190px;
 +
margin-left:5px;
 +
}
 +
.menu_list {
 +
position:fixed;top:20px;}
 +
.menu_head {
 +
padding: 5px 10px;
 +
cursor: pointer;
 +
position: relative;
 +
border:1px solid #fff;
 +
margin:1px;
 +
    font-weight:bold;
 +
    background: #ec0089;
 +
color:#fff
 +
}
 +
.menu_head:hover
 +
{
 +
cursor: pointer;
 +
color: #fff;
 +
background: #960048;
 +
text-decoration: none;
 +
background-position: 0 0;
 +
}
 +
.menu_body {
 +
display:block;
 +
}
 +
.menu_children {
 +
background: #aaa;
 +
cursor: pointer;
 +
padding: 5px 10px;
 +
font-size:12px;
 +
border-top: 1px solid #fff;
 +
border-bottom: 1px solid #fff;
 +
border-left: 1px solid #fff;
 +
border-right: 1px solid #fff;
 +
}
 +
.menu_body a{
 +
  display:block;
 +
  color:#fff;
 +
  padding-left:10px;
 +
  font-weight:bold;
 +
  text-decoration:none;
 +
}
 +
.menu_children:hover{
 +
cursor: pointer;
 +
  color: #fff;
 +
  text-decoration:none;
 +
  background:#777777;
 +
  }
 +
 +
</style>
 +
<div style="float:left;">
 +
  <div id="secondpane"> <!--Code for menu starts here-->
 +
<p class="menu_head">In this page</p>
 +
<div class="menu_body">
 +
        <div class="menu_children">
 +
<a href="#BioBrick">BioBrick</a>
 +
        </div>
 +
        <div class="menu_children">
 +
<a href="#Agarose_Gel_Electrophoresis">Agarose Gel Electrophoresis</a>
 +
        </div>
 +
      </div>
 +
  </div>      <!--Code for menu ends here-->
 +
</div>
 +
</html>
 +
<div id="text-content">
=BioBrick=
=BioBrick=
===Favorite Tianjin 2012 iGEM Team Parts===
===Favorite Tianjin 2012 iGEM Team Parts===
<html>
<html>
 +
<table border="0"
 +
cellpadding="5"
 +
style="border:2px solid #760044;">
 +
<tr>
<tr>
-
<td>1</td>
+
<th style="background:#f680c4;">&nbsp;</th>
-
<td>Working</td>
+
<th style="background:#f680c4;">&nbsp;</th>
-
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821001">BBa_K821001</a></td>
+
<th style="background:#f680c4;">Name</th>
-
<td>Reporter</td>
+
<th style="background:#f680c4;">Type</th>
-
<td>RFP Reporter with o-RBS</td>
+
<th style="background:#f680c4;">Description</th>
-
<td>Qinfeng Wu, Bin Jia</td>
+
<th style="background:#f680c4;">Designer</th>
-
<td>1059</td>
+
<th style="background:#f680c4;">Length</th>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="background:#fabfe1;">1</td>
 +
<td style="background:#fabfe1;">W</td>
 +
<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821001">BBa_K821001</a></td>
 +
<td style="background:#fabfe1;">Reporter</td>
 +
<td style="background:#fabfe1;">RFP Reporter with o-RBS</td>
 +
<td style="background:#fabfe1;">Qinfeng Wu, Bin Jia</td>
 +
<td style="background:#fabfe1;">1059</td>
</tr>
</tr>
<tr>
<tr>
<td>2</td>
<td>2</td>
-
<td>Working</td>
+
<td>W</td>
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821002">BBa_K821002</a></td>
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821002">BBa_K821002</a></td>
<td>Coding</td>
<td>Coding</td>
Line 24: Line 108:
<tr>
<tr>
-
<td>3</td>
+
<td style="background:#fabfe1;">3</td>
-
<td>Working</td>
+
<td style="background:#fabfe1;">W</td>
-
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821012">BBa_K821012</a></td>
+
<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821012">BBa_K821012</a></td>
-
<td>Generator</td>
+
<td style="background:#fabfe1;">Generator</td>
-
<td>PBad(promoter)+orthogonal 16S RNA</td>
+
<td style="background:#fabfe1;">PBad(promoter)+orthogonal 16S RNA</td>
-
<td>Jinlai Zhang, Dongchang Qin</td>
+
<td style="background:#fabfe1;">Jinlai Zhang, Dongchang Qin</td>
-
<td>3226</td>
+
<td style="background:#fabfe1;">3226</td>
</tr>
</tr>
 +
</table>
</html>
</html>
===Tianjin 2012 iGEM Team Parts Sandbox===
===Tianjin 2012 iGEM Team Parts Sandbox===
<html>
<html>
 +
<table border="0"
 +
cellpadding="5"
 +
style="border:2px solid #760044;">
 +
<tr>
<tr>
-
<td>1</td>
+
<th style="background:#f680c4;">&nbsp;</th>
-
<td>Working</td>
+
<th style="background:#f680c4;">&nbsp;</th>
-
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
+
<th style="background:#f680c4;">Name</th>
-
<td>Coding</td>
+
<th style="background:#f680c4;">Type</th>
-
<td>16S DNA sequence from rrnB</td>
+
<th style="background:#f680c4;">Description</th>
-
<td>Jinlai Zhang, Dongchang Qin</td>
+
<th style="background:#f680c4;">Designer</th>
-
<td>1894</td>
+
<th style="background:#f680c4;">Length</th>
 +
</tr>
 +
 
 +
<tr>
 +
<td style="background:#fabfe1;">1</td>
 +
<td style="background:#fabfe1;">W</td>
 +
<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
 +
<td style="background:#fabfe1;">Coding</td>
 +
<td style="background:#fabfe1;">16S DNA sequence from rrnB</td>
 +
<td style="background:#fabfe1;">Jinlai Zhang, Dongchang Qin</td>
 +
<td style="background:#fabfe1;">1894</td>
</tr>
</tr>
<tr>
<tr>
<td>2</td>
<td>2</td>
-
<td>Working</td>
+
<td>W</td>
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821004">BBa_K821004</a></td>
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821004">BBa_K821004</a></td>
<td>Regulatory</td>
<td>Regulatory</td>
Line 57: Line 156:
<tr>
<tr>
-
<td>3</td>
+
<td style="background:#fabfe1;">3</td>
-
<td>Working</td>
+
<td style="background:#fabfe1;">W</td>
-
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821005">BBa_K821005</a></td>
+
<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821005">BBa_K821005</a></td>
-
<td>Reporter</td>
+
<td style="background:#fabfe1;">Reporter</td>
-
<td>CmR label from pKD3</td>
+
<td style="background:#fabfe1;">CmR label from pKD3</td>
-
<td>Qinfeng Wu, Bin Jia</td>
+
<td style="background:#fabfe1;">Qinfeng Wu, Bin Jia</td>
-
<td>1014</td>
+
<td style="background:#fabfe1;">1014</td>
</tr>
</tr>
<tr>
<tr>
<td>4</td>
<td>4</td>
-
<td>Working</td>
+
<td>W</td>
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821006">BBa_K821006</a></td>
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821006">BBa_K821006</a></td>
<td>Project</td>
<td>Project</td>
Line 77: Line 176:
<tr>
<tr>
-
<td>5</td>
+
<td style="background:#fabfe1;">5</td>
-
<td>Working</td>
+
<td style="background:#fabfe1;">W</td>
-
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821007">BBa_K821007</a></td>
+
<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821007">BBa_K821007</a></td>
-
<td>Composite</td>
+
<td style="background:#fabfe1;">Composite</td>
-
<td>PL(promoter)+orthogonal 16S RNA</td>
+
<td style="background:#fabfe1;">PL(promoter)+orthogonal 16S RNA</td>
-
<td>Qinfeng Wu, Bin Jia</td>
+
<td style="background:#fabfe1;">Qinfeng Wu, Bin Jia</td>
-
<td>1957</td>
+
<td style="background:#fabfe1;">1957</td>
</tr>
</tr>
<tr>
<tr>
<td>6</td>
<td>6</td>
-
<td>Working</td>
+
<td>W</td>
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821008">BBa_K821008</a></td>
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821008">BBa_K821008</a></td>
<td>Composite</td>
<td>Composite</td>
Line 97: Line 196:
<tr>
<tr>
-
<td>7</td>
+
<td style="background:#fabfe1;">7</td>
-
<td>Working</td>
+
<td style="background:#fabfe1;">W</td>
-
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821009">BBa_K821009</a></td>
+
<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821009">BBa_K821009</a></td>
-
<td>Composite</td>
+
<td style="background:#fabfe1;">RBS</td>
-
<td>Orthogonal RBS 1</td>
+
<td style="background:#fabfe1;">Orthogonal RBS 1</td>
-
<td>Qinfeng Wu, Bin Jia</td>
+
<td style="background:#fabfe1;">Qinfeng Wu, Bin Jia</td>
-
<td>6</td>
+
<td style="background:#fabfe1;">6</td>
</tr>
</tr>
<tr>
<tr>
<td>8</td>
<td>8</td>
-
<td>Working</td>
+
<td>W</td>
-
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
+
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821010">BBa_K821010</a></td>
-
<td>Coding</td>
+
<td>Composite</td>
-
<td>16S DNA sequence from rrnB</td>
+
<td>PLacI+Native RBS+RFP+Native RBS+GFP+Terminator</td>
-
<td>Jinlai Zhang, Dongchang Qin</td>
+
<td>Qinfeng Wu, Bin Jia </td>
-
<td>1894</td>
+
<td>1833</td>
</tr>
</tr>
<tr>
<tr>
-
<td>1</td>
+
<td style="background:#fabfe1;">9</td>
-
<td>Working</td>
+
<td style="background:#fabfe1;">W</td>
-
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
+
<td style="background:#fabfe1;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
-
<td>Coding</td>
+
<td style="background:#fabfe1;">Composite</td>
-
<td>16S DNA sequence from rrnB</td>
+
<td style="background:#fabfe1;">PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator </td>
-
<td>Jinlai Zhang, Dongchang Qin</td>
+
<td style="background:#fabfe1;">Jinlai Zhang, Dongchang Qin</td>
-
<td>1894</td>
+
<td style="background:#fabfe1;">1827</td>
</tr>
</tr>
 +
</table>
</html>
</html>
-
=Agarose gel electrophoresis=
+
 
 +
=Agarose Gel Electrophoresis=
[[file:TJU12-data13.JPG|thumb|left|7.14 t7 rbs' crtE]][[file:TJU12-data14.JPG|thumb|none|7.31 16s' RFP-GFP AMP]]
[[file:TJU12-data13.JPG|thumb|left|7.14 t7 rbs' crtE]][[file:TJU12-data14.JPG|thumb|none|7.31 16s' RFP-GFP AMP]]
Line 148: Line 249:
-
[[file:TJU12-data11.JPG|thumb|left|9.16 yeast cPCR 2]][[file:TJU12-data12.JPG|thumb|none|81 16s GFP & RFP]]
+
[[file:TJU12-data11.JPG|thumb|left|9.16 yeast cPCR 2]][[file:TJU12-data12.JPG|thumb|none|8.01 16s GFP & RFP]]
 +
 
 +
 
 +
 

Latest revision as of 13:30, 24 October 2012

BioBrick

Favorite Tianjin 2012 iGEM Team Parts

    Name Type Description Designer Length
1 W BBa_K821001 Reporter RFP Reporter with o-RBS Qinfeng Wu, Bin Jia 1059
2 W BBa_K821002 Coding Orthogonal 16S DNA sequence from rrnB Qinfeng Wu, Bin Jia 1894
3 W BBa_K821012 Generator PBad(promoter)+orthogonal 16S RNA Jinlai Zhang, Dongchang Qin 3226

Tianjin 2012 iGEM Team Parts Sandbox

    Name Type Description Designer Length
1 W BBa_K821003 Coding 16S DNA sequence from rrnB Jinlai Zhang, Dongchang Qin 1894
2 W BBa_K821004 Regulatory PBad Promoter Jinlai Zhang, Dongchang Qin 1324
3 W BBa_K821005 Reporter CmR label from pKD3 Qinfeng Wu, Bin Jia 1014
4 W BBa_K821006 Project KanR label from pKD3 Jinlai Zhang, Dongchang Qin 1304
5 W BBa_K821007 Composite PL(promoter)+orthogonal 16S RNA Qinfeng Wu, Bin Jia 1957
6 W BBa_K821008 Composite PLtet+orthogonal 16S RNA Jinlai Zhang, Dongchang Qin 1956
7 W BBa_K821009 RBS Orthogonal RBS 1 Qinfeng Wu, Bin Jia 6
8 W BBa_K821010 Composite PLacI+Native RBS+RFP+Native RBS+GFP+Terminator Qinfeng Wu, Bin Jia 1833
9 W BBa_K821003 Composite PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator Jinlai Zhang, Dongchang Qin 1827


Agarose Gel Electrophoresis

7.14 t7 rbs' crtE
7.31 16s' RFP-GFP AMP


8.03 pSB1C3 K3 Digestion
8.16 16s' mutate to remove EcoRI


8.17 16S' pSB1C3
8.17 Mutate the RBS of Amp using PYES2


8.17 Yeast Assembly using pSB3C5R & pSB3K3
8.18 pBad-16S' remove EcorI, PSB1C3


9.2 6I 6G Gel extraction and verification
9.2 6I,6G P16S Gel extraction and verification


9.2 P16S digestion
9.16 yeast cPCR 1


9.16 yeast cPCR 2
8.01 16s GFP & RFP




Retrieved from "http://2012.igem.org/Team:Tianjin/Data"