Team:LMU-Munich/Data/crustpromoters

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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Crust promoter evaluation

The figure below shows the result of the evaluation of the three promoters (P_cgeA, P_cotV, P_cotYZ) for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 pSB_Bs3C-luxABCDE]. For each promoter two independent clones were evaluated three times. To link the promoter activities to the growth phase, the optical density (OD₆₀₀) was measured throughout the whole experiment.

Protein distribution in spore coat of Bacillus subtilis

Fig. 1: The result of the measurement of the three promoters P_cgeA, P_cotV and P_cotYZ fused to the lux operon. The left vertical axis displays the luminescence divided by the OD₆₀₀ and the left one shows the OD₆₀₀. On the horizontal axis the time is illustrated. The continuous lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.

The native promoters of cotZ, P_cotYZ and P_cotV, show a clear signal late in stationary phase, as expected. P_cotYZ is activated two hours earlier than P_cotV and both promoters are active for about 12 hours. In contrast, P_cgeA does not show any activity.

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