Team:LMU-Munich/Data

From 2012.igem.org

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{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo2.jpg}}
{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo2.jpg}}
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[[File:Data_banner.jpg|620px|link=]]
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[[File:Data banner.resized WORDS.JPG|620px|link=]]
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=Data=
 
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Here you will find all of our project data.
 
[[Image:BacillusBioBrickBox.png|right|100px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
[[Image:BacillusBioBrickBox.png|right|100px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
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====Evaluation of ''B. subtilis'' vectors====
====Evaluation of ''B. subtilis'' vectors====
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">Seven novel vectors were constructed and four already proven to work in ''B. subtilis''</p>
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|<p align="justify">Seven novel vectors were constructed and four already proven to work in ''B. subtilis''.</p>
|[[File:LacZ plate.png|right|100px|link=Team:LMU-Munich/Data/Vectors]]
|[[File:LacZ plate.png|right|100px|link=Team:LMU-Munich/Data/Vectors]]
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====Constitutive ''Bacillus'' Promoters====
====Constitutive ''Bacillus'' Promoters====
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">Three novel ''B. subtilis'' promoter BioBricks ('''P<sub>''liaG''</sub>''', '''P<sub>''veg''</sub>''', '''P<sub>''lepA''</sub>''') were constructed and functionality verified by luminescence and β-galactosidase assays.</p>
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|<p align="justify">Three novel ''B. subtilis'' promoter BioBricks (P<sub>''liaG''</sub>, P<sub>''veg''</sub>, P<sub>''lepA''</sub>) were constructed and functionality verified by luminescence and β-galactosidase assays.</p>
|[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]]
|[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]]
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====Inducible ''Bacillus'' Promoters====
====Inducible ''Bacillus'' Promoters====
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">A novel antibiotic-inducible promoter,'''P<sub>''liaI''</sub>''', was constructed and carefully evaluated.</p>
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|<p align="justify">A novel antibiotic-inducible promoter, P<sub>''liaI''</sub>, was constructed and thoroughly evaluated.</p>
|[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]]
|[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]]
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===Crust Promoter Evaluation===
===Crust Promoter Evaluation===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">Three sporulation-dependent promoters (blablabla) were constructed, two of which show the expected behavior.</p>
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|<p align="justify">Three sporulation-dependent promoters (P<sub>''cotYZ''</sub>, P<sub>''cotV''</sub>, P<sub>''cgeA''</sub>) were constructed, two of which show the expected behavior.</p>
|[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]]
|[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]]
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===GFP-'''Sporo'''bead Evaluation===
===GFP-'''Sporo'''bead Evaluation===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">They glow! GFP was successfully displayed on the spore crust as demonstreted by fluorescence microscopy</p>
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|<p align="justify">They glow! GFP was successfully displayed on the spore crust as demonstrated by fluorescence microscopy.</p>
|[[File:Sporobead Constructs Dada sheet preview.png|200px|link=Team:LMU-Munich/Data/gfp_spore]]
|[[File:Sporobead Constructs Dada sheet preview.png|200px|link=Team:LMU-Munich/Data/gfp_spore]]
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==='''Sporo'''bead Purification===
==='''Sporo'''bead Purification===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells: Lysosome is the best!</p>
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|<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells: Lysozyme is the best!</p>
|[[File:Purification preview.png|200px|link=Team:LMU-Munich/Data/Sporepurification]]
|[[File:Purification preview.png|200px|link=Team:LMU-Munich/Data/Sporepurification]]
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===Knockouts of germination genes ===
===Knockouts of germination genes ===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">We successfully prevented spore germination by multiple gene knockouts</p>
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|<p align="justify">We successfully prevented spore germination by multiple gene knockouts.</p>
|[[File:LMU Knockout previewii.jpg|200px|link=Team:LMU-Munich/Data/Knockout]]
|[[File:LMU Knockout previewii.jpg|200px|link=Team:LMU-Munich/Data/Knockout]]
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</div>
</div>
<div class="box">
<div class="box">
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===Suicide Switch ===
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==='''Suicide'''Switch ===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
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|<p align="justify">Death upon germination: We developed a novel safety strategy as a back-up to knockouts.</p>
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|<p align="justify">Death upon germination: We developed a novel safety strategy as a back-up to the gene knockouts.</p>
|[[File:PlatereaderSuicideSwitch1.jpg|200px|link=Team:LMU-Munich/Data/Suicideswitch]]
|[[File:PlatereaderSuicideSwitch1.jpg|200px|link=Team:LMU-Munich/Data/Suicideswitch]]
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{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
|<p align="justify">
|<p align="justify">
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The story of trying to clone a toxine from ''E. coli'' in ''E. coli'': They die dammit (not a surprise, isn´t it?!
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The story of trying to clone a toxin from ''E. coli'' in ''E. coli'': They die, dammit! (...not a surprise, really...)
</p>
</p>
|[[File:MazFtrashcan.jpg|200px|link=Team:LMU-Munich/Data/MazF]]
|[[File:MazFtrashcan.jpg|200px|link=Team:LMU-Munich/Data/MazF]]
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{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
|<p align="justify">
|<p align="justify">
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All constructs involving xylose-inducible promoters refused cloning or evaluation (Sorry Grohningen...!).
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All constructs involving xylose-inducible promoters refused cloning or evaluation (sorry, Groningen...!).
</p>
</p>
|[[File:100px-D-Xylose Keilstrich.png|100px|link=Team:LMU-Munich/Data/Pxyl]]
|[[File:100px-D-Xylose Keilstrich.png|100px|link=Team:LMU-Munich/Data/Pxyl]]

Latest revision as of 14:31, 22 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo2.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde

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