Team:LMU-Munich/Data

From 2012.igem.org

(Difference between revisions)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo2.jpg}}
{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo2.jpg}}
-
[[File:Data_banner.jpg|620px|link=]]
+
[[File:Data banner.resized WORDS.JPG|620px|link=]]
-
 
-
 
-
=Data=
 
-
 
-
Here you will find all of our project data.
 
[[Image:BacillusBioBrickBox.png|right|100px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
[[Image:BacillusBioBrickBox.png|right|100px|link=https://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks]]
Line 18: Line 13:
====Evaluation of ''B. subtilis'' vectors====
====Evaluation of ''B. subtilis'' vectors====
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
-
|<p align="justify">Seven novel vectors were constructed and four already proven to work in ''B. subtilis''</p>
+
|<p align="justify">Seven novel vectors were constructed and four already proven to work in ''B. subtilis''.</p>
|[[File:LacZ plate.png|right|100px|link=Team:LMU-Munich/Data/Vectors]]
|[[File:LacZ plate.png|right|100px|link=Team:LMU-Munich/Data/Vectors]]
|-
|-
Line 36: Line 31:
====Constitutive ''Bacillus'' Promoters====
====Constitutive ''Bacillus'' Promoters====
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
-
|<p align="justify">Three novel ''B. subtilis'' promoter BioBricks ('''P<sub>''liaG''</sub>''', '''P<sub>''veg''</sub>''', '''P<sub>''lepA''</sub>''') were constructed and functionality verified by luminescence and β-galactosidase assays.</p>
+
|<p align="justify">Three novel ''B. subtilis'' promoter BioBricks (P<sub>''liaG''</sub>, P<sub>''veg''</sub>, P<sub>''lepA''</sub>) were constructed and functionality verified by luminescence and β-galactosidase assays.</p>
|[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]]
|[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]]
|-
|-
Line 45: Line 40:
====Inducible ''Bacillus'' Promoters====
====Inducible ''Bacillus'' Promoters====
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
-
|<p align="justify">A novel antibiotic-inducible promoter,'''P<sub>''liaI''</sub>''', was constructed and carefully evaluated.</p>
+
|<p align="justify">A novel antibiotic-inducible promoter, P<sub>''liaI''</sub>, was constructed and thoroughly evaluated.</p>
|[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]]
|[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]]
|-
|-
Line 61: Line 56:
===Crust Promoter Evaluation===
===Crust Promoter Evaluation===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
-
|<p align="justify">Three sporulation-dependent promoters (blablabla) were constructed, two of which show the expected behavior.</p>
+
|<p align="justify">Three sporulation-dependent promoters (P<sub>''cotYZ''</sub>, P<sub>''cotV''</sub>, P<sub>''cgeA''</sub>) were constructed, two of which show the expected behavior.</p>
|[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]]
|[[File:Promotoren.png|200px|link=Team:LMU-Munich/Data/crustpromoters]]
|-
|-
Line 71: Line 66:
===GFP-'''Sporo'''bead Evaluation===
===GFP-'''Sporo'''bead Evaluation===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
-
|<p align="justify">They glow! GFP was successfully displayed on the spore crust as demonstreted by fluorescence microscopy</p>
+
|<p align="justify">They glow! GFP was successfully displayed on the spore crust as demonstrated by fluorescence microscopy.</p>
|[[File:Sporobead Constructs Dada sheet preview.png|200px|link=Team:LMU-Munich/Data/gfp_spore]]
|[[File:Sporobead Constructs Dada sheet preview.png|200px|link=Team:LMU-Munich/Data/gfp_spore]]
|-
|-
Line 81: Line 76:
==='''Sporo'''bead Purification===
==='''Sporo'''bead Purification===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
-
|<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells: Lysosome is the best!</p>
+
|<p align="justify">We compared different ways of purifying ''Bacillus'' spores from vegetative cells: Lysozyme is the best!</p>
|[[File:Purification preview.png|200px|link=Team:LMU-Munich/Data/Sporepurification]]
|[[File:Purification preview.png|200px|link=Team:LMU-Munich/Data/Sporepurification]]
|-
|-
Line 99: Line 94:
===Knockouts of germination genes ===
===Knockouts of germination genes ===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
-
|<p align="justify">We successfully prevented spore germination by multiple gene knockouts</p>
+
|<p align="justify">We successfully prevented spore germination by multiple gene knockouts.</p>
|[[File:LMU Knockout previewii.jpg|200px|link=Team:LMU-Munich/Data/Knockout]]
|[[File:LMU Knockout previewii.jpg|200px|link=Team:LMU-Munich/Data/Knockout]]
|-
|-
Line 106: Line 101:
</div>
</div>
<div class="box">
<div class="box">
-
===Suicide Switch ===
+
==='''Suicide'''Switch ===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
-
|<p align="justify">Death upon germination: We developed a novel safety strategy as a back-up to knockouts.</p>
+
|<p align="justify">Death upon germination: We developed a novel safety strategy as a back-up to the gene knockouts.</p>
|[[File:PlatereaderSuicideSwitch1.jpg|200px|link=Team:LMU-Munich/Data/Suicideswitch]]
|[[File:PlatereaderSuicideSwitch1.jpg|200px|link=Team:LMU-Munich/Data/Suicideswitch]]
|-
|-
Line 124: Line 119:
===β-galactosidase assay of Inverter with ''lacZα'' ===
===β-galactosidase assay of Inverter with ''lacZα'' ===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
-
|<p align="justify">β-galactosidase assay of Inverter with ''lacZα'' dependent on Arabinose concentrations</p>
+
|<p align="justify">It works! Invert the output of your promoter of choice.</p>
|[[File:LMU Inverter graph.png|200px|link=Team:LMU-Munich/Data/Inverter]]
|[[File:LMU Inverter graph.png|200px|link=Team:LMU-Munich/Data/Inverter]]
|-
|-
Line 141: Line 136:
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
|<p align="justify">
|<p align="justify">
-
Unexpected restriction sites combined with no activity at all</p>
+
Unexpected restriction sites combined with no activity at all - what a bummer!</p>
|[[File:LMU cgea preview.jpg|200px|link=Team:LMU-Munich/Data/cgeA]]
|[[File:LMU cgea preview.jpg|200px|link=Team:LMU-Munich/Data/cgeA]]
|-
|-
Line 152: Line 147:
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
|<p align="justify">
|<p align="justify">
-
The story of trying to clone a toxine from ''E. coli'' in ''E. coli''
+
The story of trying to clone a toxin from ''E. coli'' in ''E. coli'': They die, dammit! (...not a surprise, really...)
</p>
</p>
|[[File:MazFtrashcan.jpg|200px|link=Team:LMU-Munich/Data/MazF]]
|[[File:MazFtrashcan.jpg|200px|link=Team:LMU-Munich/Data/MazF]]
Line 161: Line 156:
<div class="box">
<div class="box">
-
===Xylose-promoters===
+
===Xylose promoters===
{| "width=100%" style="text-align:center;"|
{| "width=100%" style="text-align:center;"|
|<p align="justify">
|<p align="justify">
-
All constructs involving Xylose-inducible promoter refused cloning or evaluation.
+
All constructs involving xylose-inducible promoters refused cloning or evaluation (sorry, Groningen...!).
</p>
</p>
|[[File:100px-D-Xylose Keilstrich.png|100px|link=Team:LMU-Munich/Data/Pxyl]]
|[[File:100px-D-Xylose Keilstrich.png|100px|link=Team:LMU-Munich/Data/Pxyl]]

Latest revision as of 14:31, 22 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo2.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde