Team:Utah State
From 2012.igem.org
(Difference between revisions)
Cwbarentine (Talk | contribs) |
Cwbarentine (Talk | contribs) |
||
Line 445: | Line 445: | ||
<div style="height: 1000px;"> | <div style="height: 1000px;"> | ||
- | <div style="height: | + | <div style="height: 650px; border: 10px solid #d6d3d3; width1:1020px; z-index: 10;"> |
<div style="height:750px"> | <div style="height:750px"> | ||
- | <div style="border-bottom: 10px solid #d6d3d3; width1:1020px; height:250px;"> | + | <div style="border-bottom: 10px solid #d6d3d3; width1:1020px; height:250px; z-index: 10;"> |
<div class="bucket1"> | <div class="bucket1"> | ||
Line 459: | Line 459: | ||
</li> | </li> | ||
- | < | + | <p style="list-style: none;"> |
- | + | Team Successes | |
<br> | <br> | ||
- | <ul> | + | <ul style="list-style: none;"> |
<li>First ever spider silk BioBrick parts</li> | <li>First ever spider silk BioBrick parts</li> | ||
<li>First spun spider silk fiber from composite BioBrick part</li> | <li>First spun spider silk fiber from composite BioBrick part</li> | ||
<li>Improved His-tag for better protein purification</li> | <li>Improved His-tag for better protein purification</li> | ||
<li>First spider silk GFP fusion protein from BioBrick parts</li> | <li>First spider silk GFP fusion protein from BioBrick parts</li> | ||
- | |||
</ul> | </ul> | ||
+ | </p> | ||
+ | |||
- | |||
</div> | </div> | ||
Line 514: | Line 514: | ||
</div> | </div> | ||
</div> | </div> | ||
+ | </div> | ||
</div> | </div> | ||
Revision as of 08:29, 17 October 2012
![USU 2012](https://static.igem.org/mediawiki/2012/f/f0/Igem-Banner-2.png)
![](https://static.igem.org/mediawiki/igem.org/4/4b/Img1.png)
Spider silk is the strongest known biomaterial, with a large variety of potential applications. These potential applications include artificial tendons and ligaments, biomedical sutures, athletic gear, parachute cords, air bags, and other yet discovered products which require a high tensile strength with amazing extendability. Spiders, however, cannot be farmed because they are territorial and cannibalistic. Thus, an alternative to producing spider silk must be found. We aim to engineer spider silk genes into E. coli to produce this highly valuable product. Spider silk production in bacteria has been limited due to the highly repetitive nature of the amino acids in the spider silk protein. To overcome this obstacle, we are using various synthetic biology techniques to boost spider silk protein production and increase cellular fitness. After successful production, spider silk protein is artificially spun into usable fibers and tested for mechanical and physical properties.
Team Successes
- First ever spider silk BioBrick parts
- First spun spider silk fiber from composite BioBrick part
- Improved His-tag for better protein purification
- First spider silk GFP fusion protein from BioBrick parts