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Team:RHIT/Notebook
From 2012.igem.org
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| - | <img src="https://static.igem.org/mediawiki/igem.org/8/87/Banner_only2.png" width="100%"/> | + | <a href="https://2012.igem.org/Team:RHIT"><img src="https://static.igem.org/mediawiki/igem.org/8/87/Banner_only2.png" width="100%"/></a> |
<div id="logged_out"> | <div id="logged_out"> | ||
<a href="https://2012.igem.org/Main_Page">iGEM Home</a> | <a href="https://2012.igem.org/Main_Page">iGEM Home</a> | ||
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<h3><i>S. Cerevisiae</i></h3> | <h3><i>S. Cerevisiae</i></h3> | ||
<p>BY4741 (ATCC #201388): <i>MATa his3-?0 leu2-?0 met15-?0 ura3-?0</i></p><br /> | <p>BY4741 (ATCC #201388): <i>MATa his3-?0 leu2-?0 met15-?0 ura3-?0</i></p><br /> | ||
| - | <p>BY4742 (ATCC #201389): <i> | + | <p>BY4742 (ATCC #201389): <i>MATalpha his3-?0 leu2-?0 lys2-?0 ura3-?0</i></p><br /><br /> |
<h2>Lab Protocols</h2> | <h2>Lab Protocols</h2> | ||
<p>Transformations were carried out on competent E.Coli using the procedure available on the <a href="http://www.neb.com/nebecomm/products/protocol119.asp">NEB website</a>.</p><br /><br /> | <p>Transformations were carried out on competent E.Coli using the procedure available on the <a href="http://www.neb.com/nebecomm/products/protocol119.asp">NEB website</a>.</p><br /><br /> | ||
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<div class="rhit-fourthSheet" id="rhit-fourthSheet"> | <div class="rhit-fourthSheet" id="rhit-fourthSheet"> | ||
| - | The official RHIT Safety page can be found <a href="https://2012.igem.org/Team:RHIT/Safety">here | + | The official RHIT Safety page can be found <a href="https://2012.igem.org/Team:RHIT/Safety">here</a>. |
<h2>Researcher, Public, and Environmental Safety</h2> | <h2>Researcher, Public, and Environmental Safety</h2> | ||
<p>The Rose-Hulman iGEM team has considered safety a top priority since the early stages of project development. | <p>The Rose-Hulman iGEM team has considered safety a top priority since the early stages of project development. | ||
As a result, projects that could pose a significant risk were dismissed. | As a result, projects that could pose a significant risk were dismissed. | ||
Our laboratory space is considered a Basic Biosafety Level 1 laboratory but it does have a few characteristics of higher categories. | Our laboratory space is considered a Basic Biosafety Level 1 laboratory but it does have a few characteristics of higher categories. | ||
| - | It has controlled access, biohazard signs and waste disposal bins, and an autoclave. | + | It has controlled access, biohazard signs and waste disposal bins, and an autoclave. Other safety features are demonstrated below.<br /></p> |
| - | All participating students received standard safety and good laboratory practice training as part of their academic laboratory course-work. | + | <div align="center"><img src="https://static.igem.org/mediawiki/igem.org/2/2a/Safety_pics.png" width="60%"></div><br /> |
| + | <p>All participating students received standard safety and good laboratory practice training as part of their academic laboratory course-work. | ||
The Instructor provided more specific safety instruction, as necessary. | The Instructor provided more specific safety instruction, as necessary. | ||
Proper attire was worn at all times in the laboratory. Gloves and glasses were donned as warranted. | Proper attire was worn at all times in the laboratory. Gloves and glasses were donned as warranted. | ||
| - | The only organisms used in our project are common laboratory strains of < | + | The only organisms used in our project are common laboratory strains of <i>Saccharomyces cerevisiae</i> (BY4741 and BY4742) and <i>Escherichia coli</i> (NEB5alpa), |
which are both considered Risk Group 1 microorganisms according to the Laboratory Biosafety Manual published by the World Health Organization. | which are both considered Risk Group 1 microorganisms according to the Laboratory Biosafety Manual published by the World Health Organization. | ||
Group 1 organisms are defined as “no or low individual and community risk, a microorganism that is unlikely to cause human or animal disease.” | Group 1 organisms are defined as “no or low individual and community risk, a microorganism that is unlikely to cause human or animal disease.” | ||
Latest revision as of 03:57, 4 October 2012
