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Team:Northwestern/Modeling
From 2012.igem.org
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<p>The purpose of our modeled system is to tune this system as a specific pH-sensing lysis device for releasing our phytase into the stomach. We aim to characterize the interaction between the H+/Cl- antiporter and the chloride-induced lysis cassette in our E. coli chassis. This model examines the effects of varied promoter strengths for the ClC-ec1 antiporter, as well as the plausibility of utilizing the system for nutritional purposes. | <p>The purpose of our modeled system is to tune this system as a specific pH-sensing lysis device for releasing our phytase into the stomach. We aim to characterize the interaction between the H+/Cl- antiporter and the chloride-induced lysis cassette in our E. coli chassis. This model examines the effects of varied promoter strengths for the ClC-ec1 antiporter, as well as the plausibility of utilizing the system for nutritional purposes. | ||
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| + | <p>We start with a model cell that has entered the stomach. In this high pH environment, protons begin to leak into the cell. For our purposes, this flux of H+ ions into the cell is modeled by simple diffusion. | ||
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Revision as of 03:52, 4 October 2012
Modeling
Overview
The purpose of our modeled system is to tune this system as a specific pH-sensing lysis device for releasing our phytase into the stomach. We aim to characterize the interaction between the H+/Cl- antiporter and the chloride-induced lysis cassette in our E. coli chassis. This model examines the effects of varied promoter strengths for the ClC-ec1 antiporter, as well as the plausibility of utilizing the system for nutritional purposes.
We start with a model cell that has entered the stomach. In this high pH environment, protons begin to leak into the cell. For our purposes, this flux of H+ ions into the cell is modeled by simple diffusion.
