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Team:Queens Canada/Notebook/Week4
From 2012.igem.org
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| + | <tr class="tableizer-firstrow"><th>Date</th><th>Protocol</th><th>People</th><th>DNA (if relevant)</th><th>Quantities and Parameters (if relevant)</th></tr> <tr><td>May-22</td><td>Heat Shock Transformation</td><td>Kevin and Friends</td><td>BBa_J04450 - RFP</td><td> </td></tr> <tr><td> </td><td>Prepared Antibiotic plates</td><td>Kevin and Friends</td><td> </td><td>Added 25 uL of chloramphenicol </td></tr> <tr><td>May-23</td><td>Heat Shock Transformation</td><td>Victor, Aaron, Hope, David Faisal</td><td>BBa_J04450 - RFP</td><td> </td></tr> <tr><td>May-24</td><td>Liquid Cultures</td><td>Victor, Kevin, Faisal, Maggie</td><td>BBa_J04450 - RFP</td><td>Added 160 mL of absolute ethanol into Solution 1 </td></tr> <tr><td>May-25</td><td>Miniprep</td><td>Victor, Kevin, Faisal, David, Phillip</td><td>RFP</td><td>Four trials were minipreped</td></tr></table> | ||
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<h1> Monday May 21</h1> <p> <h2> Today was Victoria Day, a statutory holiday in Canada, so we took the day off to rest and relax and prepare for the week ahead!</p> </h2> <h1>Tuesday May 22</h1> <h2> <p> First day in lab hurray! </p> | <h1> Monday May 21</h1> <p> <h2> Today was Victoria Day, a statutory holiday in Canada, so we took the day off to rest and relax and prepare for the week ahead!</p> </h2> <h1>Tuesday May 22</h1> <h2> <p> First day in lab hurray! </p> | ||
Latest revision as of 03:50, 4 October 2012
Notebook - Week 4
| Date | Protocol | People | DNA (if relevant) | Quantities and Parameters (if relevant) |
|---|---|---|---|---|
| May-22 | Heat Shock Transformation | Kevin and Friends | BBa_J04450 - RFP | |
| Prepared Antibiotic plates | Kevin and Friends | Added 25 uL of chloramphenicol | ||
| May-23 | Heat Shock Transformation | Victor, Aaron, Hope, David Faisal | BBa_J04450 - RFP | |
| May-24 | Liquid Cultures | Victor, Kevin, Faisal, Maggie | BBa_J04450 - RFP | Added 160 mL of absolute ethanol into Solution 1 |
| May-25 | Miniprep | Victor, Kevin, Faisal, David, Phillip | RFP | Four trials were minipreped |
Monday May 21
Today was Victoria Day, a statutory holiday in Canada, so we took the day off to rest and relax and prepare for the week ahead!
Tuesday May 22
First day in lab hurray!
In the morning we went over lab training/safety orientation, as well as completed WHMIS training necessary to work in the lab. In the afternoon we cleaned up our assigned lab bench in preparation for our work in the lab tomorrow.
Wednesday May 23
This morning we worked mainly on primer design in preparation for PCR work. Kevin also met with one of our advisors, Dr. Allingham, in order to discuss modelling protein designs. In the afternoon, we tested different protocols on transformation of the Red Fluorescent Protein (RFP) BioBrick into LB plates with chloramphenicol resistance. Four trials were incubated at 37 degrees Celsius overnight.
Thursday May 24
We spent the majority of the day working on sponsorship, following up on emails sent. We also spent some time in the lab. We got red colonies in the liquid medium shown as a cloudy red liquid, and we moved on to miniprep (isolating the plasmid from the colonies). We spent our time in the lab today preparing for the miniprep procedure tomorrow.
