"
Team:Queens Canada/Notebook/Week4
From 2012.igem.org
(Difference between revisions)
| (2 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
<html> | <html> | ||
<head> | <head> | ||
| - | + | <style type="text/css"> | |
| + | table.tableizer-table {border: 1px solid #CCC; font-size: 12px;} .tableizer-table td {padding: 4px; margin: 3px; border: 1px solid #ccc;} | ||
| + | .tableizer-table th {background-color: #104E8B; color: #FFF; font-weight: bold;} | ||
| + | </style> | ||
<style type="text/css"> | <style type="text/css"> | ||
#NotebookNav .menu .primary-menu #w3 a{ | #NotebookNav .menu .primary-menu #w3 a{ | ||
| Line 10: | Line 13: | ||
background: none repeat scroll 0 0 #00438F; | background: none repeat scroll 0 0 #00438F; | ||
} | } | ||
| - | + | #navtoggle | |
| + | { | ||
| + | height:75px; | ||
| + | width:75px; | ||
| + | position:absolute; | ||
| + | left:-75 px; | ||
| + | margin-left: -75px; | ||
| + | margin-bottom: -75px; | ||
| + | margin-top: 35 px; | ||
| + | text-align: center; | ||
| + | background-color: maroon; | ||
| + | border-radius: 5px 0px 0px 5px; | ||
| + | } | ||
| + | #navtoggle a | ||
| + | { | ||
| + | color: white; | ||
| + | font-size: 1.1em; | ||
| + | vertical-align: middle; | ||
| + | } | ||
</style> | </style> | ||
| Line 17: | Line 38: | ||
<div id="labcontent"> | <div id="labcontent"> | ||
| - | |||
| - | |||
| - | |||
<div id="NotebookTitle"> | <div id="NotebookTitle"> | ||
| - | <p>Notebook - Week | + | <p>Notebook - Week 4</p> |
</div> | </div> | ||
<div id="notebookmenu"> | <div id="notebookmenu"> | ||
| Line 65: | Line 83: | ||
</script> | </script> | ||
| - | <div id="protocolcontent | + | <div id="protocolcontent"> |
| - | + | ||
| + | <table class="tableizer-table"> | ||
| + | <tr class="tableizer-firstrow"><th>Date</th><th>Protocol</th><th>People</th><th>DNA (if relevant)</th><th>Quantities and Parameters (if relevant)</th></tr> <tr><td>May-22</td><td>Heat Shock Transformation</td><td>Kevin and Friends</td><td>BBa_J04450 - RFP</td><td> </td></tr> <tr><td> </td><td>Prepared Antibiotic plates</td><td>Kevin and Friends</td><td> </td><td>Added 25 uL of chloramphenicol </td></tr> <tr><td>May-23</td><td>Heat Shock Transformation</td><td>Victor, Aaron, Hope, David Faisal</td><td>BBa_J04450 - RFP</td><td> </td></tr> <tr><td>May-24</td><td>Liquid Cultures</td><td>Victor, Kevin, Faisal, Maggie</td><td>BBa_J04450 - RFP</td><td>Added 160 mL of absolute ethanol into Solution 1 </td></tr> <tr><td>May-25</td><td>Miniprep</td><td>Victor, Kevin, Faisal, David, Phillip</td><td>RFP</td><td>Four trials were minipreped</td></tr></table> | ||
| + | </div> | ||
| + | <div id="notebookcontent"> | ||
<h1> Monday May 21</h1> <p> <h2> Today was Victoria Day, a statutory holiday in Canada, so we took the day off to rest and relax and prepare for the week ahead!</p> </h2> <h1>Tuesday May 22</h1> <h2> <p> First day in lab hurray! </p> | <h1> Monday May 21</h1> <p> <h2> Today was Victoria Day, a statutory holiday in Canada, so we took the day off to rest and relax and prepare for the week ahead!</p> </h2> <h1>Tuesday May 22</h1> <h2> <p> First day in lab hurray! </p> | ||
<p>In the morning we went over lab training/safety orientation, as well as completed WHMIS training necessary to work in the lab. In the afternoon we cleaned up our assigned lab bench in preparation for our work in the lab tomorrow.</p> <h1> Wednesday May 23 </h1> <h2> <p> This morning we worked mainly on primer design in preparation for PCR work. Kevin also met with one of our advisors, Dr. Allingham, in order to discuss modelling protein designs. In the afternoon, we tested different protocols on transformation of the Red Fluorescent Protein (RFP) BioBrick into LB plates with chloramphenicol resistance. Four trials were incubated at 37 degrees Celsius overnight. </h2> </p> <h1> Thursday May 24 </h1> <h2> <p> We spent the majority of the day working on sponsorship, following up on emails sent. We also spent some time in the lab. We got red colonies in the liquid medium shown as a cloudy red liquid, and we moved on to miniprep (isolating the plasmid from the colonies). We spent our time in the lab today preparing for the miniprep procedure tomorrow. </h2> </p> <h1> Friday May 25 </h1> <h2> Today we worked more on sponsorship in the morning, and got into the lab in the afternoon. We performed the miniprep procedure on the liquid colonies, which involved adding a lot of different solutions as well as centrifugation. We isolated BBa_J04450, and stored it in the fridge at -20 degrees Celsius. We also looked at the red colonies under the microscope, and they did fluoresce. After work, Kevin and the rest of the team involved in SynthetiQ brainstormed some ideas for videos. </p> </h2> | <p>In the morning we went over lab training/safety orientation, as well as completed WHMIS training necessary to work in the lab. In the afternoon we cleaned up our assigned lab bench in preparation for our work in the lab tomorrow.</p> <h1> Wednesday May 23 </h1> <h2> <p> This morning we worked mainly on primer design in preparation for PCR work. Kevin also met with one of our advisors, Dr. Allingham, in order to discuss modelling protein designs. In the afternoon, we tested different protocols on transformation of the Red Fluorescent Protein (RFP) BioBrick into LB plates with chloramphenicol resistance. Four trials were incubated at 37 degrees Celsius overnight. </h2> </p> <h1> Thursday May 24 </h1> <h2> <p> We spent the majority of the day working on sponsorship, following up on emails sent. We also spent some time in the lab. We got red colonies in the liquid medium shown as a cloudy red liquid, and we moved on to miniprep (isolating the plasmid from the colonies). We spent our time in the lab today preparing for the miniprep procedure tomorrow. </h2> </p> <h1> Friday May 25 </h1> <h2> Today we worked more on sponsorship in the morning, and got into the lab in the afternoon. We performed the miniprep procedure on the liquid colonies, which involved adding a lot of different solutions as well as centrifugation. We isolated BBa_J04450, and stored it in the fridge at -20 degrees Celsius. We also looked at the red colonies under the microscope, and they did fluoresce. After work, Kevin and the rest of the team involved in SynthetiQ brainstormed some ideas for videos. </p> </h2> | ||
| + | </div> | ||
<script> | <script> | ||
$("#notebook").click(function () { | $("#notebook").click(function () { | ||
| - | $("#protocolcontent").fadeOut( | + | $("#protocolcontent").fadeOut(400, function () { |
| - | $("#notebookcontent").fadeIn( | + | $("#notebookcontent").fadeIn(400); |
}); | }); | ||
return false; | return false; | ||
| Line 83: | Line 105: | ||
<script> | <script> | ||
$("#protocols").click(function () { | $("#protocols").click(function () { | ||
| - | $("#notebookcontent").fadeOut( | + | $("#notebookcontent").fadeOut(400, function () { |
| - | $("#protocolcontent").fadeIn( | + | $("#protocolcontent").fadeIn(400); |
}); | }); | ||
return false; | return false; | ||
| Line 116: | Line 138: | ||
</div> | </div> | ||
</body> | </body> | ||
| - | |||
Latest revision as of 03:50, 4 October 2012
Control
Notebook - Week 4
| Date | Protocol | People | DNA (if relevant) | Quantities and Parameters (if relevant) |
|---|---|---|---|---|
| May-22 | Heat Shock Transformation | Kevin and Friends | BBa_J04450 - RFP | |
| Prepared Antibiotic plates | Kevin and Friends | Added 25 uL of chloramphenicol | ||
| May-23 | Heat Shock Transformation | Victor, Aaron, Hope, David Faisal | BBa_J04450 - RFP | |
| May-24 | Liquid Cultures | Victor, Kevin, Faisal, Maggie | BBa_J04450 - RFP | Added 160 mL of absolute ethanol into Solution 1 |
| May-25 | Miniprep | Victor, Kevin, Faisal, David, Phillip | RFP | Four trials were minipreped |
Monday May 21
Today was Victoria Day, a statutory holiday in Canada, so we took the day off to rest and relax and prepare for the week ahead!
Tuesday May 22
First day in lab hurray!
In the morning we went over lab training/safety orientation, as well as completed WHMIS training necessary to work in the lab. In the afternoon we cleaned up our assigned lab bench in preparation for our work in the lab tomorrow.
Wednesday May 23
This morning we worked mainly on primer design in preparation for PCR work. Kevin also met with one of our advisors, Dr. Allingham, in order to discuss modelling protein designs. In the afternoon, we tested different protocols on transformation of the Red Fluorescent Protein (RFP) BioBrick into LB plates with chloramphenicol resistance. Four trials were incubated at 37 degrees Celsius overnight.
Thursday May 24
We spent the majority of the day working on sponsorship, following up on emails sent. We also spent some time in the lab. We got red colonies in the liquid medium shown as a cloudy red liquid, and we moved on to miniprep (isolating the plasmid from the colonies). We spent our time in the lab today preparing for the miniprep procedure tomorrow.
