Team:NYU Gallatin/Project/Transforming

From 2012.igem.org

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<li class="menu-312"><a href="/Team:NYU_Gallatin/Safety" title="Our commitment to safety.">Safety</a></li>
<li class="menu-312"><a href="/Team:NYU_Gallatin/Safety" title="Our commitment to safety.">Safety</a></li>
<li class="menu-313"><a href="/Team:NYU_Gallatin/Attributions" title="Give credit where credit is due.">Attributions</a></li>
<li class="menu-313"><a href="/Team:NYU_Gallatin/Attributions" title="Give credit where credit is due.">Attributions</a></li>
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<li class="menu-306 last"><a href="https://igem.org/Team.cgi?year=2012&team_name=NYU_Gallatin" title="Official iGEM 2012 profile.">Profile</a></li>
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<li class="menu-306 last"><a href="https://igem.org/Team.cgi?year=2012&amp;team_name=NYU_Gallatin" title="Official iGEM 2012 profile.">Profile</a></li>
</ul></div> <!-- /#main-menu -->
</ul></div> <!-- /#main-menu -->
<div id="page-wrapper"><div id="page">
<div id="page-wrapper"><div id="page">
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     <ul class="menu clearfix"><li class="first last expanded active-trail"><a href="/Team:NYU_Gallatin/Project" title="" class="active-trail">Project</a><ul class="menu clearfix"><li class="first leaf"><a href="/Team:NYU_Gallatin/Project/Cloning" title="">Cloning</a></li>
     <ul class="menu clearfix"><li class="first last expanded active-trail"><a href="/Team:NYU_Gallatin/Project" title="" class="active-trail">Project</a><ul class="menu clearfix"><li class="first leaf"><a href="/Team:NYU_Gallatin/Project/Cloning" title="">Cloning</a></li>
<li class="leaf active-trail"><a href="/Team:NYU_Gallatin/Project/Transforming" title="We transformed stuff." class="active-trail active">Transforming</a></li>
<li class="leaf active-trail"><a href="/Team:NYU_Gallatin/Project/Transforming" title="We transformed stuff." class="active-trail active">Transforming</a></li>
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<li class="leaf"><a href="/Team:NYU_Gallatin/Project/Characterization" title="">Characterizing</a></li>
<li class="leaf"><a href="/Team:NYU_Gallatin/Project/Growing" title="">Growing</a></li>
<li class="leaf"><a href="/Team:NYU_Gallatin/Project/Growing" title="">Growing</a></li>
<li class="leaf"><a href="/Team:NYU_Gallatin/Project/Designing" title="">Designing</a></li>
<li class="leaf"><a href="/Team:NYU_Gallatin/Project/Designing" title="">Designing</a></li>
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   <div class="content clearfix">
   <div class="content clearfix">
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     <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><p><img src="http://farm9.staticflickr.com/8454/8052039349_973bb2a2b1_m.jpg" class="border" />   <img src="http://farm9.staticflickr.com/8452/8052046690_74e9f03b01_n.jpg" class="border" /></p>
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     <center><img style="border: solid black 1px; margin-bottom: 20px;" src="http://farm9.staticflickr.com/8311/8052386481_6e29d9d7c0_c.jpg" width=683 /></center><div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><p><img src="http://farm9.staticflickr.com/8452/8052046690_74e9f03b01_n.jpg" class="border" style="float: left; margin-right: 20px; margin-bottom: 15px;" />With regard to transformation, electroporation is well-known for its higher cell wall penetration rate and its efficiency on higher-organism cells, mammalian cells in particular. We used electroporation on E. Coli cells, adapting and improving available electroporation protocols to optimize transformed colony yield. <img src="http://farm9.staticflickr.com/8454/8052039349_973bb2a2b1_m.jpg" class="border" style="float: right; margin-left: 20px; margin-bottom: 15px; margin-top: 20px;" /> We hypothesized that corrected voltage and cell preparation procedures would allow us to successfully transform.  Acetobacter Xylinum through electroporation. Tests included E.Coli and Acetobacter Xylinum transformation for RFP and GFP expression. Pure Acetobacter Xylinum cultures were also grown in media for several weeks and a chart has been prepared to describe and detail growth patterns. Using a spectrophotometer, light absorbency readings were taken regularly to allow for the collection of daily data. Through this measurement technique, we also determined optimal growth phases for the preparation of Acetobacter Xylinum as an electro-competent cell. </p>
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<p><img src="http://farm9.staticflickr.com/8317/8052051236_7e6f368694_n.jpg" class="border" />   <img src="http://farm9.staticflickr.com/8182/8052044117_7b58cb9ea3_n.jpg" class="border" /></p>
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<p><img src="http://farm9.staticflickr.com/8317/8052051236_7e6f368694_n.jpg" class="border" style="clear: left" />   <img src="http://farm9.staticflickr.com/8182/8052044117_7b58cb9ea3_n.jpg" class="border" /></p>
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<p></p><center><img src="http://farm9.staticflickr.com/8450/8052524036_2d2900d98a_z.jpg" class="border" /></center>
<h1>Protocols</h1>
<h1>Protocols</h1>
<h2>Modified E. Coli and Acetobacter Electroporation Protocol</h2>
<h2>Modified E. Coli and Acetobacter Electroporation Protocol</h2>
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<li>Incubate the culture for 1 hour at 37 degrees with gentle agitation</li>
<li>Incubate the culture for 1 hour at 37 degrees with gentle agitation</li>
<li>After incubation, take 200mL of the sample and plate onto appropriate agar, use beads to spread the sample around the plate</li>
<li>After incubation, take 200mL of the sample and plate onto appropriate agar, use beads to spread the sample around the plate</li>
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</ol></div></div></div>  </div>
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</ol><p></p><center><img src="http://farm8.staticflickr.com/7121/7864390690_87fb603e4b_n.jpg" class="border" />   <img src="http://farm9.staticflickr.com/8431/7864392046_b300d7dd16_n.jpg" class="border" /></center>
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</div></div></div>  </div>
    
    
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           <tr class="odd views-row-first">
           <tr class="odd views-row-first">
                   <td class="views-field views-field-field-sub-team" >
                   <td class="views-field views-field-field-sub-team" >
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             Sarah<br />Transformation          </td>
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             Transformation          </td>
                   <td class="views-field views-field-created" >
                   <td class="views-field views-field-created" >
             Aug/10/2012          </td>
             Aug/10/2012          </td>
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           <tr class="even">
           <tr class="even">
                   <td class="views-field views-field-field-sub-team" >
                   <td class="views-field views-field-field-sub-team" >
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             Sarah<br />Transformation          </td>
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             Transformation          </td>
                   <td class="views-field views-field-created" >
                   <td class="views-field views-field-created" >
             Aug/07/2012          </td>
             Aug/07/2012          </td>
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           <tr class="odd">
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             Sarah<br />Transformation          </td>
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             Transformation          </td>
                   <td class="views-field views-field-created" >
                   <td class="views-field views-field-created" >
             Aug/04/2012          </td>
             Aug/04/2012          </td>
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           <tr class="even">
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             Sarah<br />Transformation          </td>
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             Transformation          </td>
                   <td class="views-field views-field-created" >
                   <td class="views-field views-field-created" >
             Aug/03/2012          </td>
             Aug/03/2012          </td>
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           <tr class="odd">
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                   <td class="views-field views-field-field-sub-team" >
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             Sarah<br />Transformation          </td>
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             Transformation          </td>
                   <td class="views-field views-field-created" >
                   <td class="views-field views-field-created" >
             Aug/02/2012          </td>
             Aug/02/2012          </td>
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             Sarah<br />Transformation          </td>
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             Transformation          </td>
                   <td class="views-field views-field-created" >
                   <td class="views-field views-field-created" >
             Aug/01/2012          </td>
             Aug/01/2012          </td>
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           <tr class="odd">
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             Sarah<br />Transformation          </td>
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             Transformation          </td>
                   <td class="views-field views-field-created" >
                   <td class="views-field views-field-created" >
             Jul/30/2012          </td>
             Jul/30/2012          </td>
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           <tr class="even views-row-last">
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             Transformation          </td>
                   <td class="views-field views-field-created" >
                   <td class="views-field views-field-created" >
             Jul/20/2012          </td>
             Jul/20/2012          </td>

Latest revision as of 03:29, 4 October 2012