Team:Penn State/Modeling
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+ | |||
+ | |||
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+ | <div id="navitabs"> | ||
+ | <h2 class="hide">Sample navigation menu:</h2> | ||
+ | <a class="navitab"https://2012.igem.org/Team:Penn_State">Home</a> | ||
+ | <span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a> | ||
+ | <span class="hide"> | </span> <a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a> | ||
+ | <span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a> | ||
+ | <span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Modeling</a> | ||
+ | <span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a> | ||
+ | <span class="hide"> | </span> <a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a> | ||
+ | </div> | ||
+ | |||
+ | <div id="desc"> | ||
+ | <h2>Bidirectional Promoters</h2> | ||
+ | <p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce | ||
+ | the expected proteins. Some bidirectional promoters are known to exist, but which way they promote | ||
+ | and the degree of expression has not been quantified. This project will test the directionality of | ||
+ | several BioBrick promoters to answer these questions. </p> | ||
+ | </div> | ||
+ | |||
+ | <div id="main"> | ||
+ | <h1>BiDirectional Promoter Project Data</h1> | ||
+ | <p>Here we can see some of the data collected for the Bidirectional Promoter Project. The data shows the fluorescence of GFP and RFP from different promoters collected by the TCAN. | ||
+ | </p> | ||
+ | <p>GFP Data for all of the samples tested and the average of those samples for a given construct. The promoters tested so far are <a href="http://partsregistry.org/Part:BBa_J23102">BBa_J23102</a>, and three custom palindromic promoters. | ||
+ | </p> | ||
+ | <a href="https://static.igem.org/mediawiki/2012/3/3c/PennState_BiDir_GFP_All_Graph.jpg"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/3/3c/PennState_BiDir_GFP_All_Graph.jpg" width="575px" height="275px"> | ||
+ | </a> | ||
+ | <br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2012/a/a9/PennState_BiDir_GFP_Combined_Graph.jpg"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/a/a9/PennState_BiDir_GFP_Combined_Graph.jpg" width="380px" height="270px"> | ||
+ | </a> | ||
+ | <br/> | ||
+ | <p>RFP Data for all of the samples tested and the average of those samples is shown below.</p> | ||
+ | </p> | ||
+ | <a href="https://static.igem.org/mediawiki/2012/b/b6/PennState_BiDir_RFP_All_Graph.jpg"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/b/b6/PennState_BiDir_RFP_All_Graph.jpg" width="575px" height="275px"> | ||
+ | </a> | ||
+ | <br/> | ||
+ | <a href="https://static.igem.org/mediawiki/2012/9/9f/PennState_BiDir_RFP_Combined_Graph.jpg"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/9/9f/PennState_BiDir_RFP_Combined_Graph.jpg" width="380px" height="270px"> | ||
+ | </a> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div id="sidebar"> | ||
+ | <h3>Check these Out!</h3> | ||
+ | <ul class="sidelink"> | ||
+ | <li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li> | ||
+ | </ul> | ||
+ | <h3>Picture Gallery</h3> | ||
+ | <img class="photo" src="../images/test.jpg" height="100" width="130" alt="" /> | ||
+ | <h3>Projects</h3> | ||
+ | <ul> | ||
+ | <li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div id="footer"> | ||
+ | <p><strong>© Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </body> | ||
+ | </html> |
Revision as of 03:17, 4 October 2012
Bidirectional Promoters
Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce the expected proteins. Some bidirectional promoters are known to exist, but which way they promote and the degree of expression has not been quantified. This project will test the directionality of several BioBrick promoters to answer these questions.
BiDirectional Promoter Project Data
Here we can see some of the data collected for the Bidirectional Promoter Project. The data shows the fluorescence of GFP and RFP from different promoters collected by the TCAN.
GFP Data for all of the samples tested and the average of those samples for a given construct. The promoters tested so far are BBa_J23102, and three custom palindromic promoters.
RFP Data for all of the samples tested and the average of those samples is shown below.