Team:Lethbridge/notebook

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<li><a href="https://2012.igem.org/Team:Lethbridge"><img src="http://reamsbottom.net/igem/images/homeicon.png"/></a></li>
<li><a href="https://2012.igem.org/Team:Lethbridge"><img src="http://reamsbottom.net/igem/images/homeicon.png"/></a></li>
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<li><a class="active" href="https://2012.igem.org/Team:Lethbridge/projectoverview">The Project</a></li>
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<li><a href="https://2012.igem.org/Team:Lethbridge/projectoverview">The Project</a></li>
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<li><a href="#">Results</a></li>
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<li><a href="https://2012.igem.org/Team:Lethbridge/results">Results</a></li>
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<li><a href="https://2012.igem.org/Team:Lethbridge/notebook">Notebook</a></li>
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<li><a class="active" href="https://2012.igem.org/Team:Lethbridge/notebook">Notebook</a></li>
<li><a href="https://2012.igem.org/Team:Lethbridge/parts">Parts</a></li>
<li><a href="https://2012.igem.org/Team:Lethbridge/parts">Parts</a></li>
<li><a href="https://2012.igem.org/Team:Lethbridge/ethics">Human Practices</a></li>
<li><a href="https://2012.igem.org/Team:Lethbridge/ethics">Human Practices</a></li>
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<h2 class="pagetitle">June 2012</h2>
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<p>Content Here</p>
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<p>Read More...</p>
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<h2>July 2012</h2>
<h2>July 2012</h2>
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<p>Date: July 13th, 2012</p>
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<p>Date: July 13, 2012</p>
<p>Experimenters: Jenn & Boris</p>
<p>Experimenters: Jenn & Boris</p>
<p>Transformation of Multiple Kill Switches</p>
<p>Transformation of Multiple Kill Switches</p>
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<p>Objective: To extract DNA from kit plates and transform into E. coli DH5</p>
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<p>Objective: To extract DNA from kit plates and transform into E. coli DH5a</p>
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<p>
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<li>K112806 from 2012 kit plate 4 well 6M </li>
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<li>K112808 from 2012 kit plate 3 well 6G </li>
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<p><a href="http://reamsbottom.net/igem/docs/July2012Notebook.pdf">Read More Entries from July by clicking here...</a></p>  
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<li>K117000 from 2012 kit plate 2 well 14D </li>
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<li>K124014 from 2012 kit plate 3 well 20K </li>
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<p>Procedure:</p>
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<p>Extraction of DNA from kit plates</p>
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<ul>
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<li>10 µL of Milli-Q H2O and extract parts from the kit plates
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Transformation into E. coli DH5</li>
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<li>Add 2 µL of DNA extract to 20 µL of E. coli DH5</li>
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<li>Incubate on ice for 30 min; 30 sec in water bath at 42˚C</li>
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<li>Immediately on ice for 5 min</li>
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<li>Add 400 µL of SOC media & resuspend</li>
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<li>Incubate for 1 hr at 37˚C in shaker</li>
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<li>Plate 200 µL of transformed E. coli on correct antibiotic plate</li>
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<li>Leave overnight in incubator at 37˚C</li>
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<p><a href="http://reamsbottom.net/igem/docs/JulyKillSwitchNotebook.pdf">Read More Entries from July by clicking here...</a></p>  
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<h2>August and September 2012</h2>
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<h2>August 2012</h2>
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<p>Content Here</p>  
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<p>Date: August 2, 2012</p>
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<p><a href="http://reamsbottom.net/igem/docs/July30-SeptemberKillswitchNotebook.pdf">Read More Entries from August by clicking here...</a></p>  
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<p>Experimenters: Iain</p>
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<p>Objective: To assemble the following DNA parts</p>
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<p><a href="http://reamsbottom.net/igem/docs/August2012Notebook.pdf">Read More Entries from August by clicking here...</a></p>  
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<h2>September 2012</h2>
<h2>September 2012</h2>
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<p>Content Here</p>  
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<p>Date: September 9, 2012</p>
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<p>Read More...</p>  
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<p>Experimenters: Iain</p>
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<p>Objective: To run a confirmation gel for each part</p>
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<p><a href="http://reamsbottom.net/igem/docs/September2012Notebook.pdf">Read More Entries from September by clicking here...</a></p>  
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Latest revision as of 03:04, 4 October 2012

2012 iGEM - University of Lethbridge

Notebook

Our notebooks document the University of Lethbridge's iGEM Team through their scientific journey

July 2012

Date: July 13, 2012

Experimenters: Jenn & Boris

Transformation of Multiple Kill Switches

Objective: To extract DNA from kit plates and transform into E. coli DH5a

Read More Entries from July by clicking here...

August 2012

Date: August 2, 2012

Experimenters: Iain

Objective: To assemble the following DNA parts

Read More Entries from August by clicking here...

September 2012

Date: September 9, 2012

Experimenters: Iain

Objective: To run a confirmation gel for each part

Read More Entries from September by clicking here...

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