Team:Alberta

From 2012.igem.org

(Difference between revisions)
 
(145 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
{{:Team:Alberta/Templates/Header}}
-
 
+
<html>
<html>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
<div class="roundBox">
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
<font size=5>
-
This is a template page. READ THESE INSTRUCTIONS.
+
Towards a Microbial Colour Wheel: Spatial Control of Gene Expression
-
</div>
+
</font>
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
+
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace. 
+
-
</div>
+
</div>
</div>
</html>
</html>
 +
<br>
-
<!-- *** End of the alert box *** -->
+
<html>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2012/b/b2/Rainbow_copy_copy_2.jpg" alt="rinbow picture" height="400" width="600"/>
 +
</html>
 +
<br>
 +
<html>
 +
<div class="underline">
 +
<font size=4>
 +
Concept
 +
</font>
 +
</div>
 +
</html>
 +
<br>
 +
<font size=2>
 +
What can we do with synthetic biology, and how easily can we do it? We picked a straightforward test: making and combining gradients of color to create patterns such as a rainbow and a color wheel. Doing this would require gaining control over several color outputs in a smoothly varying manner, and being able to pattern the inputs to create the pattern desired. Achieving this would demonstrate a degree of fine control over engineered organisms.
 +
</font>
 +
<br>
 +
<br>
-
{|align="justify"
+
<html>
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
<div class="underline">
-
|[[Image:ALBERTA|200px|right|frame]]
+
<font size=4>
-
|-
+
Approach
-
|
+
</font>
-
 
+
</div>
-
''Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
+
</html>
-
|[[Image:Alberta_team.png|right|frame|Your team picture]]
+
<br>
-
|-
+
<font size=2>
-
|
+
We used and improved the great colors from Uppsala 2011 as outputs. We first tried putting them under IPTG control, which was successful, but the dynamic range was disappointing. Next, we developed and tested a means of controlling copy number, which allowed us to both increase the dynamic range of control, and also create a permanently settable switch through plasmid loss. (Incidentally, this could make a great safety switch for environmental release.) Finally, we designed a system that would use these parts to create a rainbow of color, although we didn’t have time to finish putting the pieces together.
-
|align="center"|[[Team:Alberta | Team Alberta]]
+
</font>
-
|}
+
<br>
 +
<br>
-
<!--- The Mission, Experiments --->
+
<html>
-
 
+
<div class="roundBox">
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
<font size=4> 
-
!align="center"|[[Team:Alberta|Home]]
+
2012 Sponsors
-
!align="center"|[[Team:Alberta/Team|Team]]
+
</font>
-
!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Alberta Official Team Profile]
+
</div>
-
!align="center"|[[Team:Alberta/Project|Project]]
+
</html>
-
!align="center"|[[Team:Alberta/Parts|Parts Submitted to the Registry]]
+
<html>
-
!align="center"|[[Team:Alberta/Modeling|Modeling]]
+
<div class="roundBox">
-
!align="center"|[[Team:Alberta/Notebook|Notebook]]
+
    <div class="roundBoxInner">
-
!align="center"|[[Team:Alberta/Safety|Safety]]
+
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.albertatechfutures.ca/Home.aspx">
-
!align="center"|[[Team:Alberta/Attributions|Attributions]]
+
<img src="https://static.igem.org/mediawiki/2012/8/8b/Alberta_innovates.jpg" alt="Alberta innovates" width="150" height="75" />
-
|}
+
</a>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://geneious.com/web/geneious/home">
 +
<img src="https://static.igem.org/mediawiki/2012/5/5e/Sponsor_geneious.png" alt="Geneious" width="150" height="75" />
 +
</a>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.idtdna.com/site">
 +
<img src="https://static.igem.org/mediawiki/2012/2/22/IDT-Logo.jpg" alt="IDT" width="150" height="75" />
 +
</a>
 +
<br>
 +
<br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.neb.ca/">
 +
<img src="https://static.igem.org/mediawiki/2012/b/bb/Bioenb.png" alt="NEB" width="150" height="75" />
 +
</a>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.osli.ca/">
 +
<img src="https://static.igem.org/mediawiki/2012/8/82/OSLI.png" alt="OSLI" width="150" height="75" />
 +
</a>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.ualberta.ca/">
 +
<img src="https://static.igem.org/mediawiki/2012/e/e9/U_of_a.jpg" alt="U oF A" width="150" height="50" />
 +
</a>
 +
    </div>
 +
</div>
 +
</html>
 +
<br>

Latest revision as of 03:01, 4 October 2012

   Team Alberta




Towards a Microbial Colour Wheel: Spatial Control of Gene Expression

               rinbow picture


Concept

What can we do with synthetic biology, and how easily can we do it? We picked a straightforward test: making and combining gradients of color to create patterns such as a rainbow and a color wheel. Doing this would require gaining control over several color outputs in a smoothly varying manner, and being able to pattern the inputs to create the pattern desired. Achieving this would demonstrate a degree of fine control over engineered organisms.

Approach

We used and improved the great colors from Uppsala 2011 as outputs. We first tried putting them under IPTG control, which was successful, but the dynamic range was disappointing. Next, we developed and tested a means of controlling copy number, which allowed us to both increase the dynamic range of control, and also create a permanently settable switch through plasmid loss. (Incidentally, this could make a great safety switch for environmental release.) Finally, we designed a system that would use these parts to create a rainbow of color, although we didn’t have time to finish putting the pieces together.

2012 Sponsors
                Alberta innovates             Geneious             IDT

                NEB             OSLI             U oF A

Retrieved from "http://2012.igem.org/Team:Alberta"