Team:Alberta

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This is a template page. READ THESE INSTRUCTIONS.
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Towards a Microbial Colour Wheel: Spatial Control of Gene Expression
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2012/b/b2/Rainbow_copy_copy_2.jpg" alt="rinbow picture" height="400" width="600"/>
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Concept
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What can we do with synthetic biology, and how easily can we do it? We picked a straightforward test: making and combining gradients of color to create patterns such as a rainbow and a color wheel. Doing this would require gaining control over several color outputs in a smoothly varying manner, and being able to pattern the inputs to create the pattern desired. Achieving this would demonstrate a degree of fine control over engineered organisms.
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Alberta_logo.png|200px|right|frame]]
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Approach
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|align="center"|[[Team:Alberta | Team Alberta]]
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We used and improved the great colors from Uppsala 2011 as outputs. We first tried putting them under IPTG control, which was successful, but the dynamic range was disappointing. Next, we developed and tested a means of controlling copy number, which allowed us to both increase the dynamic range of control, and also create a permanently settable switch through plasmid loss. (Incidentally, this could make a great safety switch for environmental release.) Finally, we designed a system that would use these parts to create a rainbow of color, although we didn’t have time to finish putting the pieces together.
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!align="center"|[[Team:Alberta|Home]]
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2012 Sponsors
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!align="center"|[[Team:Alberta/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Alberta Official Team Profile]
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!align="center"|[[Team:Alberta/Project|Project]]
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!align="center"|[[Team:Alberta/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Alberta/Modeling|Modeling]]
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!align="center"|[[Team:Alberta/Safety|Safety]]
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.albertatechfutures.ca/Home.aspx">
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!align="center"|[[Team:Alberta/Attributions|Attributions]]
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<img src="https://static.igem.org/mediawiki/2012/8/8b/Alberta_innovates.jpg" alt="Alberta innovates" width="150" height="75" />
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://geneious.com/web/geneious/home">
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<img src="https://static.igem.org/mediawiki/2012/5/5e/Sponsor_geneious.png" alt="Geneious" width="150" height="75" />
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.idtdna.com/site">
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.neb.ca/">
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<img src="https://static.igem.org/mediawiki/2012/b/bb/Bioenb.png" alt="NEB" width="150" height="75" />
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.osli.ca/">
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<img src="https://static.igem.org/mediawiki/2012/8/82/OSLI.png" alt="OSLI" width="150" height="75" />
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.ualberta.ca/">
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<img src="https://static.igem.org/mediawiki/2012/e/e9/U_of_a.jpg" alt="U oF A" width="150" height="50" />
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Latest revision as of 03:01, 4 October 2012

   Team Alberta




Towards a Microbial Colour Wheel: Spatial Control of Gene Expression

               rinbow picture


Concept

What can we do with synthetic biology, and how easily can we do it? We picked a straightforward test: making and combining gradients of color to create patterns such as a rainbow and a color wheel. Doing this would require gaining control over several color outputs in a smoothly varying manner, and being able to pattern the inputs to create the pattern desired. Achieving this would demonstrate a degree of fine control over engineered organisms.

Approach

We used and improved the great colors from Uppsala 2011 as outputs. We first tried putting them under IPTG control, which was successful, but the dynamic range was disappointing. Next, we developed and tested a means of controlling copy number, which allowed us to both increase the dynamic range of control, and also create a permanently settable switch through plasmid loss. (Incidentally, this could make a great safety switch for environmental release.) Finally, we designed a system that would use these parts to create a rainbow of color, although we didn’t have time to finish putting the pieces together.

2012 Sponsors
                Alberta innovates             Geneious             IDT

                NEB             OSLI             U oF A

Retrieved from "http://2012.igem.org/Team:Alberta"