|
|
| (14 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | {{:Team:Michigan/Template:Michigan Header}} | + | {{:Team:Michigan/Template:New}} |
| - | | + | |
| - | [[Team:Michigan/Notebook/Protocols]]
| + | |
| | | | |
| | + | <!-- No need for this |
| | {| style="color:#ffffff;background-color:#929487;" cellpadding="3" cellspacing="1" border="0" width="100%" align="center" | | {| style="color:#ffffff;background-color:#929487;" cellpadding="3" cellspacing="1" border="0" width="100%" align="center" |
| | !align="center"|[[Team:Michigan|Home]] | | !align="center"|[[Team:Michigan|Home]] |
| Line 14: |
Line 13: |
| | !align="center"|[[Team:Michigan/Attributions|Attributions]] | | !align="center"|[[Team:Michigan/Attributions|Attributions]] |
| | |} | | |} |
| - | | + | --> |
| - | <!-- *** Here is where you should enter your lab notebook! Remember to name and date your entries. Use the icons on the top left corner to embed image, bold, italicize, and so forth. For technical support, email huetone@gmail.com or ask Candy. *** -->
| + | |
| - | | + | |
| | | | |
| | <html> | | <html> |
| - | <h2>Increasing Recombinase HbiF and FimE Turnover Rate with LVA Degradation Tag </h2> | + | <div class="body"> |
| - | <p>HbiF and LVA degradation tag were ordered from IDT with the following specifications.</p> | + | <div id="basic"> |
| | | | |
| - | <p> <span style="background-color:#E0771B;font-family:Courier, monospace;">EcoRI</span></p> | + | <!-- What is this? |
| - | <p> <span style="background-color:#E0B91B;font-family:Courier, monospace;">XbaI</span></p> | + | <div class="locator"> |
| - | <p> <span style="background-color:#C8E0DB;font-family:Courier, monospace;">SpeI</span></p> | + | <p>< a href=" /Team: Michigan/Project"> Project</ a> > < a href=" /Team: Michigan/Noteboook"> Notebook</ a></p> |
| - | <p> <span style="background-color:#A9C9A4;font-family:Courier, monospace;">PstI</span></p> | + | </div> |
| - | <p> <span style="background-color:#86C67C;font-family:Courier, monospace;">AgeI</span></p>
| + | --> |
| - | <p> <span style="background-color:#CD96CD;font-family:Courier, monospace;">NgoMIV</span></p>
| + | |
| | | | |
| - | <p><br/>HbiF possesses an N-terminus in the (truncated) RFC10 format :</p>
| |
| | | | |
| - | <p style="font-family:Courier, monospace;"><span style="background-color:#E0771B">GAATTC</span> GCGGCCGC T <span style="background-color:#E0B91B;">TCTAG</span> [ATG]</p> | + | <!-- *** Here is where you should enter your lab notebook! Remember to name and date your entries. Use the icons on the top left corner to embed image, bold, italicize, and so forth. For technical support, email huetone@gmail.com or ask Candy. *** --> |
| - | | + | |
| - | <p><br/>No extra amino acids are created by this prefix, in comparison to the standard RFC25 prefix, which creates a serine and glycine. The suffix proceeds as follows:</p>
| + | |
| - | | + | |
| - | <p style="font-family:Courier, monospace;"><span style="background-color:#86C67C;">ACCGGT</span> TAAT <span style="background-color:#C8E0DB">ACTAGT</span> A GCGGCCG <span style="background-color:#A9C9A4">CTGCAG</span> </p>
| + | |
| | | | |
| - | <p>In order to properly attach the LVA degradation tag, the prefix of the tag sequence must be complementary to the suffix of Hbif. For this part, the prefix and suffix are reversed in comparison to above: the prefix is RFC25, and the suffix is RFC10, for reasons that are discussed below.</p> | + | <h2>Cloning Team Notebook</h2> |
| | + | <iframe width=100% height=800; src="https://docs.google.com/document/pub?id=1bDaN8iqQjBHllPLosoQUs9Z9MZT4Q3pjOG3M_9UMEho&embedded=true"></iframe> |
| | | | |
| | | | |
| - | <h2>Prepare FimE for protein expression</h2>
| + | </div> |
| - | <iframe width=100% height=500; src="https://docs.google.com/document/pub?id=1bDaN8iqQjBHllPLosoQUs9Z9MZT4Q3pjOG3M_9UMEho&embedded=true"></iframe>
| + | </div> |
| - | <h2>Team Reports</h2>
| + | |
| - | <iframe src="https://docs.google.com/presentation/embed?id=17czITWHCoyzbzdYCvXWmwpDbl31jJyhgNmb32IXX3aY&start=false&loop=false&delayms=8000" frameborder="0" width=60% height=400 allowfullscreen="true" mozallowfullscreen="true" webkitallowfullscreen="true"></iframe>
| + | |
| | | | |
| | </html> | | </html> |
| | | | |
| | <!-- *** End of the alert box *** --> | | <!-- *** End of the alert box *** --> |
"
