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From 2012.igem.org

Revision as of 01:09, 4 October 2012 (view source) Ashourz (Talk | contribs) ← Older edit		Latest revision as of 01:28, 4 October 2012 (view source) Leah3393 (Talk | contribs)
(One intermediate revision not shown)
Line 4:		Line 4:
	June 6		June 6
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 27:		Line 27:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	June 7		June 7
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked and glycerol stocked made		Colonies picked and glycerol stocked made
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 54:		Line 54:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page3" class='notebook_page'>		<div id="page3" class='notebook_page'>
Line 60:		Line 60:
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	June 8		June 8
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid isolation		Plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 95:		Line 95:
	</ul>		</ul>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page4" class='notebook_page'>		<div id="page4" class='notebook_page'>
Line 102:		Line 102:
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	June 11		June 11
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 125:		Line 125:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	June 12		June 12
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 146:		Line 146:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 161:		Line 161:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page5" class='notebook_page'>		<div id="page5" class='notebook_page'>
Line 167:		Line 167:
	June 13		June 13
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Incubate constitutive promoter-RBS-GFP in LB carb broth overnight @ 37*C and 220 rpm		Incubate constitutive promoter-RBS-GFP in LB carb broth overnight @ 37*C and 220 rpm
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 177:		Line 177:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	June 14		June 14
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol stock and miniprep		Glycerol stock and miniprep
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 206:		Line 206:
	Test Digestion for each plasmid: correct results received		Test Digestion for each plasmid: correct results received
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	June 15		June 15
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid isolation		Plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	prom-RBS-GFP: Concentration: 127 ng/μl	+	prom-RBS-GFP: Concentration: 127 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page6" class='notebook_page'>		<div id="page6" class='notebook_page'>
Line 232:		Line 232:
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 16		July 16
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Transformations		Transformations
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 255:		Line 255:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 17		July 17
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 301:		Line 301:
	<div id="page7" class='notebook_page'>		<div id="page7" class='notebook_page'>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 18		July 18
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol stocks made		Glycerol stocks made
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 320:		Line 320:
	<ul>		<ul>
	<li>		<li>
-	Concentration: 236.2 ng/μl	+	Concentration: 236.2 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 328:		Line 328:
	<ul>		<ul>
	<li>		<li>
-	Concentration: 92.9 ng/μl	+	Concentration: 92.9 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 336:		Line 336:
	<ul>		<ul>
	<li>		<li>
-	Concentration: 60.6 ng/μl	+	Concentration: 60.6 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 344:		Line 344:
	<ul>		<ul>
	<li>		<li>
-	Concentration: 110.9 ng/μl	+	Concentration: 110.9 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 351:		Line 351:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 366:		Line 366:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page8" class='notebook_page'>		<div id="page8" class='notebook_page'>
Line 373:		Line 373:
	Gel Extraction		Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 380:		Line 380:
	<ul>		<ul>
	<li>		<li>
-	Concentration: 10.8 ng/μl	+	Concentration: 10.8 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 388:		Line 388:
	<ul>		<ul>
	<li>		<li>
-	Concentration: 19.6 ng/μl	+	Concentration: 19.6 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 396:		Line 396:
	<ul>		<ul>
	<li>		<li>
-	Concentration: 6.2 ng/μl	+	Concentration: 6.2 ng/µl
	</li>		</li>
	</ul>		</ul>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 413:		Line 413:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 434:		Line 434:
	</ul>		</ul>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page9" class='notebook_page'>		<div id="page9" class='notebook_page'>
Line 441:		Line 441:
	July 19		July 19
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Transformations		Transformations
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 470:		Line 470:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	July 20		July 20
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 508:		Line 508:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	July 20		July 20
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol stocks &amp; plasmid isolation		Glycerol stocks &amp; plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 527:		Line 527:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 537:		Line 537:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Gel Extraction		Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pLac-TetR: Concentration: 20.5 ng/μl	+	pLac-TetR: Concentration: 20.5 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 550:		Line 550:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 562:		Line 562:
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 580:		Line 580:
	</ul>		</ul>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 20		July 20
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Repeat Transformation		Repeat Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 609:		Line 609:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 21		July 21
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock		Glycerol Stock
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 636:		Line 636:
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 655:		Line 655:
	Digestion &amp; Gel Extraction		Digestion &amp; Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 661:		Line 661:
	</li>		</li>
	<li>		<li>
-	LuxR: Concentration: 11.1 ng/μl	+	LuxR: Concentration: 11.1 ng/µl
	</li>		</li>
	<li>		<li>
-	LuxI: Concentration: 7.1 ng/μl	+	LuxI: Concentration: 7.1 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page13" class='notebook_page'>		<div id="page13" class='notebook_page'>
Line 674:		Line 674:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
Line 680:		Line 680:
	July 22		July 22
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LuxS: Concentration: 75.2 ng/μl	+	LuxS: Concentration: 75.2 ng/µl
	</li>		</li>
	<li>		<li>
-	LasI: Concentration: 90.1 ng/μl	+	LasI: Concentration: 90.1 ng/µl
	</li>		</li>
	<li>		<li>
-	RBS-YFP: Concentration: 76.4 ng/μl	+	RBS-YFP: Concentration: 76.4 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 23		July 23
	</p>		</p>
-	<br/>	+
	<p>		<p>
	DIgestion and gel extraction		DIgestion and gel extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 717:		Line 717:
	</li>		</li>
	<li>		<li>
-	LasI (E/S) Concentration: 20.3 ng/μl	+	LasI (E/S) Concentration: 20.3 ng/µl
	</li>		</li>
	<li>		<li>
-	RBS-YFP (X/P) Concentration: 18.2 ng/μl	+	RBS-YFP (X/P) Concentration: 18.2 ng/µl
	</li>		</li>
	<li>		<li>
Line 729:		Line 729:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 741:		Line 741:
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 760:		Line 760:
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 772:		Line 772:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	July 23		July 23
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol stock and plasmid isolation		Glycerol stock and plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 791:		Line 791:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 803:		Line 803:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 824:		Line 824:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 24		July 24
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LuxS: Concentration: 85.9 ng/μl	+	LuxS: Concentration: 85.9 ng/µl
	</li>		</li>
	<li>		<li>
-	LuxI: Concentration:85.7 ng/μl	+	LuxI: Concentration:85.7 ng/µl
	</li>		</li>
	<li>		<li>
-	LasI: Concentration: 224.1 ng/μl	+	LasI: Concentration: 224.1 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 847:		Line 847:
	Digestion and gel extraction		Digestion and gel extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LuxI (S/P): Concentration: 25.5 ng/μl	+	LuxI (S/P): Concentration: 25.5 ng/µl
	</li>		</li>
	<li>		<li>
-	LasI (S/P): Concentration: 30.7 ng/μl	+	LasI (S/P): Concentration: 30.7 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 859:		Line 859:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 874:		Line 874:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
Line 882:		Line 882:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 24		July 24
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 906:		Line 906:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 25		July 25
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 930:		Line 930:
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 945:		Line 945:
	Digestion and gel extraction		Digestion and gel extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pLsrA (S/P): Concentration: 20.6 ng/μl	+	pLsrA (S/P): Concentration: 20.6 ng/µl
	</li>		</li>
Line 955:		Line 955:
	<li>		<li>
-	pLuxR (S/P): Concentration: 18.7 ng/μl	+	pLuxR (S/P): Concentration: 18.7 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 961:		Line 961:
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 973:		Line 973:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 25		July 25
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock and plasmid isolation		Glycerol Stock and plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 992:		Line 992:
	<ul>		<ul>
	<li>		<li>
-	Concentration 1: 194.8 ng/μl	+	Concentration 1: 194.8 ng/µl
	</li>		</li>
	<li>		<li>
-	Concentartion 2: 117.2 ng/μl	+	Concentartion 2: 117.2 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 1,002:		Line 1,002:
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,015:		Line 1,015:
	</ul>		</ul>
	<p>		<p>
-	Tetracycle Stock: 50 ng/чl	+	Tetracycle Stock: 50 ng/?l
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page18" class='notebook_page'>		<div id="page18" class='notebook_page'>
	<p>		<p>
-	IPTG stock: 50 ng/чl	+	IPTG stock: 50 ng/?l
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 26		July 26
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,052:		Line 1,052:
	Glycerol stock and plasmid isolation		Glycerol stock and plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LuxI-RBS-YFP: Concentration: 70.8 ng/μl	+	LuxI-RBS-YFP: Concentration: 70.8 ng/µl
	</li>		</li>
	<li>		<li>
-	LasI-RBS-RFP: Concentration: 4.0 (to be repeated) ng/μl	+	LasI-RBS-RFP: Concentration: 4.0 (to be repeated) ng/µl
	</li>		</li>
	</ul>		</ul>
Line 1,064:		Line 1,064:
	Digestion and gel extraction		Digestion and gel extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,070:		Line 1,070:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 26		July 26
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,095:		Line 1,095:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 27		July 27
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol stock and plasmid isolation		Glycerol stock and plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pTetR: Concentration: 42.4 ng/μl	+	pTetR: Concentration: 42.4 ng/µl
	</li>		</li>
	<li>		<li>
-	pLasR: Concentration: 62.2 ng/μl	+	pLasR: Concentration: 62.2 ng/µl
	</li>		</li>
	<li>		<li>
-	LuxI-RBS-YFP: Concentration: 120.9 ng/μl	+	LuxI-RBS-YFP: Concentration: 120.9 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LasI-RBS-RFP: Concentration: 85 ng/μl	+	LasI-RBS-RFP: Concentration: 85 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 1,129:		Line 1,129:
	Digestion and gel extraction		Digestion and gel extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pTetR (S/P): Concentration: 17.4 ng/μl	+	pTetR (S/P): Concentration: 17.4 ng/µl
	</li>		</li>
	<li>		<li>
-	pLasR (S/P): Concentration: 23.8 ng/μl	+	pLasR (S/P): Concentration: 23.8 ng/µl
	</li>		</li>
	<li>		<li>
-	LuxI-RBS-YFP (X/P): Concentration: 13.8 ng/μl	+	LuxI-RBS-YFP (X/P): Concentration: 13.8 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LasI-RBS-RFP (X/P): Concentration: 8.9 ng/μl	+	LasI-RBS-RFP (X/P): Concentration: 8.9 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 1,150:		Line 1,150:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,172:		Line 1,172:
	Transformation:		Transformation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,181:		Line 1,181:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,187:		Line 1,187:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 27		July 27
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,213:		Line 1,213:
	Plasmid isolation		Plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LasR: Concentration: 95.8 ng/μl	+	LasR: Concentration: 95.8 ng/µl
	</li>		</li>
	<li>		<li>
-	Term-Term: Concentration: 120.1 ng/μl	+	Term-Term: Concentration: 120.1 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 1,225:		Line 1,225:
	Toggle switch test		Toggle switch test
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,240:		Line 1,240:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 28		July 28
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,265:		Line 1,265:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 28		July 28
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol stock and plasmid isolation		Glycerol stock and plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LacI: Concentration: 73.9 ng/μl	+	LacI: Concentration: 73.9 ng/µl
	</li>		</li>
	<li>		<li>
-	LacI (2): Concentration: 109.8 ng/μl	+	LacI (2): Concentration: 109.8 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page22" class='notebook_page'>		<div id="page22" class='notebook_page'>
Line 1,294:		Line 1,294:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 29		July 29
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stocks		Glycerol Stocks
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pLsrA-LuxI-RBS-YFP: Concentration: 201.6 ng/μl	+	pLsrA-LuxI-RBS-YFP: Concentration: 201.6 ng/µl
	</li>		</li>
	<li>		<li>
-	pLuxR-LasI-RBS-RFP: Concentration: 127.1 ng/μl	+	pLuxR-LasI-RBS-RFP: Concentration: 127.1 ng/µl
	</li>		</li>
	<li>		<li>
-	RBS-CFP: Concentration: 84.0 ng/μl	+	RBS-CFP: Concentration: 84.0 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 30		July 30
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pLsrA-LuxI-RBS-YFP: Concentration: 201.6 ng/μl	+	pLsrA-LuxI-RBS-YFP: Concentration: 201.6 ng/µl
	</li>		</li>
	<li>		<li>
-	pLuxR-LasI-RBS-RFP: Concentration: 127.1 ng/μl	+	pLuxR-LasI-RBS-RFP: Concentration: 127.1 ng/µl
	</li>		</li>
	<li>		<li>
-	RBS-CFP: Concentration: 84.0 ng/μl	+	RBS-CFP: Concentration: 84.0 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 1,341:		Line 1,341:
	Digestion and gel extraction		Digestion and gel extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,350:		Line 1,350:
	</li>		</li>
	<li>		<li>
-	RBS-CFP (X/P): Concentration: 10.4 ng/μl	+	RBS-CFP (X/P): Concentration: 10.4 ng/µl
	</li>		</li>
	<li>		<li>
-	pLac-LuxR (S/P): Concentration: 21.0 ng/μl	+	pLac-LuxR (S/P): Concentration: 21.0 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	</div>		</div>
Line 1,376:		Line 1,376:
	Ligation and transformation:		Ligation and transformation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,388:		Line 1,388:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 30		July 30
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Experiment 7		Experiment 7
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	150 ng/чl Tet	+	150 ng/?l Tet
	</li>		</li>
	<li>		<li>
-	200 ng/чl Tet	+	200 ng/?l Tet
	</li>		</li>
	<li>		<li>
-	300 ng/чl Tet	+	300 ng/?l Tet
	</li>		</li>
	<li>		<li>
Line 1,423:		Line 1,423:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,432:		Line 1,432:
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 31		July 31
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid isolation		Plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LuxS: Concentration: 78.1 ng/μl	+	LuxS: Concentration: 78.1 ng/µl
	</li>		</li>
	<li>		<li>
-	pLuxR-LasI-RBS-RFP: Concentration: 48.7 ng/μl	+	pLuxR-LasI-RBS-RFP: Concentration: 48.7 ng/µl
	</li>		</li>
	</ul>		</ul>
Line 1,456:		Line 1,456:
	Digestion:		Digestion:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,462:		Line 1,462:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,468:		Line 1,468:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Test Digestion:		Test Digestion:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,478:		Line 1,478:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,484:		Line 1,484:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Colonies:		Colonies:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,494:		Line 1,494:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,500:		Line 1,500:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page25" class='notebook_page'>		<div id="page25" class='notebook_page'>
Line 1,508:		Line 1,508:
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	July 31		July 31
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation:		Plasmid Isolation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	GFP: 137.5 ng/μl	+	GFP: 137.5 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	August 1, 2012:		August 1, 2012:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation:		Plasmid Isolation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pLuxR-LasI-RBS-RFP: 61.2 ng/μl	+	pLuxR-LasI-RBS-RFP: 61.2 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pLasR-RBS-CFP: 49.2 ng/μl	+	pLasR-RBS-CFP: 49.2 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion:		Digestion:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,555:		Line 1,555:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,561:		Line 1,561:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,567:		Line 1,567:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,573:		Line 1,573:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Gel Exraction:		Gel Exraction:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	GFP: 8.5 ng/μl	+	GFP: 8.5 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LacI: 12.7 ng/μl	+	LacI: 12.7 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
Line 1,596:		Line 1,596:
	Colony:		Colony:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,602:		Line 1,602:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Transformation:		Transformation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,612:		Line 1,612:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,618:		Line 1,618:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 1		August 1
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligation:		Ligation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,638:		Line 1,638:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,644:		Line 1,644:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,650:		Line 1,650:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Transformation:		Transformation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,660:		Line 1,660:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,666:		Line 1,666:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,672:		Line 1,672:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page27" class='notebook_page'>		<div id="page27" class='notebook_page'>
Line 1,681:		Line 1,681:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 2, 2012:		August 2, 2012:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies:		Colonies:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,695:		Line 1,695:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,701:		Line 1,701:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation:		Plasmid Isolation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LuxS: Concentration – 40.5 ng/μl	+	LuxS: Concentration – 40.5 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pLasR-CFP: 95.8 ng/μl	+	pLasR-CFP: 95.8 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Digestion:		Digestion:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,727:		Line 1,727:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,733:		Line 1,733:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,739:		Line 1,739:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,745:		Line 1,745:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Gel Extraction:		Gel Extraction:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LasI: Concentration – 4.1 ng/μl	+	LasI: Concentration – 4.1 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	LasR: Concentration – 5.3 ng/μl	+	LasR: Concentration – 5.3 ng/µl
	</li>		</li>
Line 1,764:		Line 1,764:
	<div id="page28" class='notebook_page'>		<div id="page28" class='notebook_page'>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pLasR-RBS-CFP – 13.4 ng/μl	+	pLasR-RBS-CFP – 13.4 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Ligation:		Ligation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,780:		Line 1,780:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,786:		Line 1,786:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,792:		Line 1,792:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 2		August 2
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies:		Colonies:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,812:		Line 1,812:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,818:		Line 1,818:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,824:		Line 1,824:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 3, 2012:		August 3, 2012:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stocks/Miniprep:		Glycerol Stocks/Miniprep:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pTet-GFP: Concentration-142.2 ng/μl	+	pTet-GFP: Concentration-142.2 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pTet-LacI: Concentration-67.2 ng/μl	+	pTet-LacI: Concentration-67.2 ng/µl
	</li>		</li>
	</div>		</div>
Line 1,854:		Line 1,854:
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pLac-GFP: Concentration-50.5 ng/μl	+	pLac-GFP: Concentration-50.5 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Digestion:		Digestion:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,870:		Line 1,870:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,876:		Line 1,876:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,882:		Line 1,882:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,888:		Line 1,888:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 3		August 3
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligation:		Ligation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,907:		Line 1,907:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,913:		Line 1,913:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,919:		Line 1,919:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Ligation:		Ligation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,929:		Line 1,929:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,935:		Line 1,935:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,941:		Line 1,941:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page30" class='notebook_page'>		<div id="page30" class='notebook_page'>
Line 1,948:		Line 1,948:
	Glycerol Stock:		Glycerol Stock:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pLuxR-LasI-RBS-RFP: Concentration – 45.1 ng/μl	+	pLuxR-LasI-RBS-RFP: Concentration – 45.1 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
-	pTetR-LuxI: Concentration – 105.1 ng/μl	+	pTetR-LuxI: Concentration – 105.1 ng/µl
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Digestion:		Digestion:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,970:		Line 1,970:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Ligation:		Ligation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,986:		Line 1,986:
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 1,995:		Line 1,995:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 4		August 4
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,016:		Line 2,016:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
-	<br/>	+
	</div>		</div>
Line 2,027:		Line 2,027:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 5		August 5
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock		Glycerol Stock
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,050:		Line 2,050:
	Transformation (repeated)		Transformation (repeated)
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,056:		Line 2,056:
	</li>		</li>
	<li>		<li>
-	<br/>	+
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 6		August 6
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,080:		Line 2,080:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,090:		Line 2,090:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Plasmid isolation		Plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,109:		Line 2,109:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Digestion and gel extraction (improperly prepared for gel)		Digestion and gel extraction (improperly prepared for gel)
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,125:		Line 2,125:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,141:		Line 2,141:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 6, 2012:		August 6, 2012:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	PCR:		PCR:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA created		RFP-LVA created
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Experiments:		Experiments:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Test mechanism shows TetR and LacI are medium strength repressors.		Test mechanism shows TetR and LacI are medium strength repressors.
Line 2,171:		Line 2,171:
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	Toggle Experiment fails to show significant changes in fluorescence protein after 2 or 6 hours.		Toggle Experiment fails to show significant changes in fluorescence protein after 2 or 6 hours.
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 7		August 7
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Glycerol Stock		Glycerol Stock
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,197:		Line 2,197:
	Plasmid isolation		Plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,218:		Line 2,218:
	Digestion and gel extraction		Digestion and gel extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,239:		Line 2,239:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,254:		Line 2,254:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,267:		Line 2,267:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 7, 2012		August 7, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock/Miniprep		Glycerol Stock/Miniprep
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI		pTet-LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-GFP		pLac-GFP
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion &amp; Gel Extraction		Digestion &amp; Gel Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI: XbaI and PstI		pTet-LacI: XbaI and PstI
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page35" class='notebook_page'>		<div id="page35" class='notebook_page'>
Line 2,305:		Line 2,305:
	pLac-GFP: SpeI and PstI		pLac-GFP: SpeI and PstI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Ligation/Transformation		Ligation/Transformation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Vector: pLac-GFP		Vector: pLac-GFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Insert: pTet-LacI		Insert: pTet-LacI
	</p>		</p>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 8		August 8
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,347:		Line 2,347:
	Repeat transformations from ligation		Repeat transformations from ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,356:		Line 2,356:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 8, 2012		August 8, 2012
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Transformations:		Transformations:
Line 2,374:		Line 2,374:
	<div id="page36" class='notebook_page'>		<div id="page36" class='notebook_page'>
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Negative control had ~20 colonies		Negative control had ~20 colonies
	</p>		</p>
-	<br/>	+
	<p>		<p>
	2:1 sample had ~ 40 colonies, we picked 2		2:1 sample had ~ 40 colonies, we picked 2
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Positive had too many colonies to count		Positive had too many colonies to count
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	PCR Reaction (Gradient)		PCR Reaction (Gradient)
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	-55, 60, and 65 degrees		-55, 60, and 65 degrees
Line 2,398:		Line 2,398:
	-55 degrees found to be most ideal		-55 degrees found to be most ideal
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Gel Extraction (of PCR)		Gel Extraction (of PCR)
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	Poor results, no DNA extracted		Poor results, no DNA extracted
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plate test for toggle switch:		Plate test for toggle switch:
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	1 tube inoculated with IPTG 10 microliters of .2mM solution		1 tube inoculated with IPTG 10 microliters of .2mM solution
Line 2,417:		Line 2,417:
	1 tube inoculated with ATC 10 microliters of 20ng/microliter		1 tube inoculated with ATC 10 microliters of 20ng/microliter
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 9		August 9
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol stocks		Glycerol stocks
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,447:		Line 2,447:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,459:		Line 2,459:
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,465:		Line 2,465:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,477:		Line 2,477:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,483:		Line 2,483:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,489:		Line 2,489:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,501:		Line 2,501:
	Gel Extraction		Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,507:		Line 2,507:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,516:		Line 2,516:
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,528:		Line 2,528:
	Innoculation: pLac-LuxR-pLuxR-LasI-RBS-RFP		Innoculation: pLac-LuxR-pLuxR-LasI-RBS-RFP
	</p>		</p>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 9, 2012		August 9, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol stock/miniprep:		Glycerol stock/miniprep:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-GFP-ST-pTet-LacI		pLac-GFP-ST-pTet-LacI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	PCR Reaction		PCR Reaction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA		RFP-LVA
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Experiment: Repressor Test Mechanism (LacI)		Experiment: Repressor Test Mechanism (LacI)
	</p>		</p>
-	<br/>	+
	<p>		<p>
	1 - Induced 10microliters of 10mM IPTG		1 - Induced 10microliters of 10mM IPTG
	</p>		</p>
-	<br/>	+
	<p>		<p>
	1 - No induction		1 - No induction
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion and Gel Extraction:		Digestion and Gel Extraction:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA: XbaI and PstI		RFP-LVA: XbaI and PstI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ampicillin Backbone: SpeI and PstI		Ampicillin Backbone: SpeI and PstI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
Line 2,590:		Line 2,590:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 10		August 10
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock		Glycerol Stock
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,607:		Line 2,607:
	Plasmid isolation		Plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,616:		Line 2,616:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,625:		Line 2,625:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,643:		Line 2,643:
	Transformation:		Transformation:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,655:		Line 2,655:
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 10, 2012		August 10, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligation/Transformation		Ligation/Transformation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA into Ampicillin backbone		RFP-LVA into Ampicillin backbone
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Inoculations:		Inoculations:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Toggle		Toggle
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas		pLas
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI		LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxR		LuxR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet		pTet
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLux		pLux
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxI		LuxI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	August 11, 2012		August 11, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid isolation		Plasmid isolation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Toggle		Toggle
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas		pLas
Line 2,724:		Line 2,724:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI		LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxR		LuxR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet		pTet
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLux		pLux
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxI		LuxI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion/Extraction		Digestion/Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxR: X and P		LuxR: X and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLux: S and P		pLux: S and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxI: S and P		LuxI: S and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasR: X and P		LasR: X and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Double Terminators (DT): X and P		Double Terminators (DT): X and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasI: X and P		LasI: X and P
	</p>		</p>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 11		August 11
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,797:		Line 2,797:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
Line 2,804:		Line 2,804:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 12		August 12
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock and Plasmid isolation		Glycerol Stock and Plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,824:		Line 2,824:
	Transformation repeat		Transformation repeat
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,833:		Line 2,833:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,845:		Line 2,845:
	Gel Extraction		Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,857:		Line 2,857:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,876:		Line 2,876:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 13		August 13
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,897:		Line 2,897:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 13, 2012		August 13, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Miniprep/Glycerol Stock		Miniprep/Glycerol Stock
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA		RFP-LVA
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestions/Extraction		Digestions/Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas: S and P		pLas: S and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI: X and P		LacI: X and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA: E and P		RFP-LVA: E and P
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Inoculations		Inoculations
Line 2,939:		Line 2,939:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Chlorabackbone		Chlorabackbone
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA		RFP-LVA
	</p>		</p>
-	<br/>	+
	<p>		<p>
	DT		DT
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 14		August 14
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock and Plasmid isolation		Glycerol Stock and Plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,976:		Line 2,976:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 2,985:		Line 2,985:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Parts Recieved		Parts Recieved
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,010:		Line 3,010:
	Streaking		Streaking
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,019:		Line 3,019:
	Ligation repeat		Ligation repeat
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,034:		Line 3,034:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 14, 2012		August 14, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Miniprep/Glycerol Stock		Miniprep/Glycerol Stock
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Chlorabackbone		Chlorabackbone
	</p>		</p>
-	<br/>	+
	<p>		<p>
	DT		DT
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion/Extraction:		Digestion/Extraction:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	DT: X and P		DT: X and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Chlorabackbone: E and P		Chlorabackbone: E and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Toggle: E and P		Toggle: E and P
Line 3,075:		Line 3,075:
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Ligation/Transformation:		Ligation/Transformation:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	DT into LuxR		DT into LuxR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	TetR into pLux		TetR into pLux
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasI into pTet		LasI into pTet
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasR into DT		LasR into DT
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas into LacI		pLas into LacI
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pLac into LuxI		pLac into LuxI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 15		August 15
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,125:		Line 3,125:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,137:		Line 3,137:
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,150:		Line 3,150:
	Testing		Testing
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,184:		Line 3,184:
	Streaking		Streaking
	</p>		</p>
-	<br/>	+
	<p>		<p>
	BBa_K091109		BBa_K091109
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,197:		Line 3,197:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 15, 2012		August 15, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page47" class='notebook_page'>		<div id="page47" class='notebook_page'>
Line 3,215:		Line 3,215:
	LuxR-DT: 15 colonies, picked 1 colony		LuxR-DT: 15 colonies, picked 1 colony
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLux-TetR: 35 colonies, picked 1		pLux-TetR: 35 colonies, picked 1
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasI: Negative control growth; unsuccessful		pTet-LasI: Negative control growth; unsuccessful
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasR-DT: 25 colonies, picked 1		LasR-DT: 25 colonies, picked 1
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas-LacI: 0 growth		pLas-LacI: 0 growth
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LuxI: 15 colonies, picked 1		pLac-LuxI: 15 colonies, picked 1
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasI into pTet		LasI into pTet
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasI		pTet-LasI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas-LacI		pLas-LacI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Inoculations		Inoculations
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Chlorabackbone		Chlorabackbone
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 16		August 16
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies		Colonies
Line 3,282:		Line 3,282:
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,291:		Line 3,291:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,309:		Line 3,309:
	Glycerol Stock and plasmid isolation		Glycerol Stock and plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,324:		Line 3,324:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,345:		Line 3,345:
	Testing		Testing
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,414:		Line 3,414:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,435:		Line 3,435:
	Gel Extraction		Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,447:		Line 3,447:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,453:		Line 3,453:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
Line 3,460:		Line 3,460:
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 16, 2012		August 16, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Miniprep/Glycerol Stock		Miniprep/Glycerol Stock
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxR-DT		LuxR-DT
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLux-TetR		pLux-TetR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasR-DT		LasR-DT
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacI		pLac-LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Chlorabackbone		Chlorabackbone
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Transformation Results		Transformation Results
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Negative controls had too much growth; results thrown out		Negative controls had too much growth; results thrown out
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion/Extraction		Digestion/Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxR-DT: S and P		LuxR-DT: S and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLux-TetR: X and P		pLux-TetR: X and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Chlorabackbone: E and P		Chlorabackbone: E and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligations/Transformation		Ligations/Transformation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxR-DT and pLux-TetR		LuxR-DT and pLux-TetR
Line 3,524:		Line 3,524:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Chlorabackbone with Toggle Switch		Chlorabackbone with Toggle Switch
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 17		August 17
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock		Glycerol Stock
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,556:		Line 3,556:
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,574:		Line 3,574:
	Digestion and Gel Extraction		Digestion and Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,595:		Line 3,595:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,631:		Line 3,631:
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,646:		Line 3,646:
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 17, 2012		August 17, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas with LacI		pLas with LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet with LasI		pTet with LasI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Inoculations		Inoculations
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas		pLas
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI		LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet		pTet
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac		pLac
	</p>		</p>
-	<br/>	+
	<p>		<p>
	GFP		GFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA		RFP-LVA
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasI		LasI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Bba_K081014 Kit Plate 3 Well 12O Resistance AK; RFP		Bba_K081014 Kit Plate 3 Well 12O Resistance AK; RFP
	</p>		</p>
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page55" class='notebook_page'>		<div id="page55" class='notebook_page'>
Line 3,718:		Line 3,718:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 18		August 18
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Innoculations		Innoculations
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,741:		Line 3,741:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 18, 2012		August 18, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colony Picking		Colony Picking
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxR-DT-pLux-TetR: 30 colonies, 1 picked		LuxR-DT-pLux-TetR: 30 colonies, 1 picked
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Toggle Switch in Chlorabackbone: 4 colonies, 1 picked		Toggle Switch in Chlorabackbone: 4 colonies, 1 picked
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas with LacI: negative control growth; results thrown out		pLas with LacI: negative control growth; results thrown out
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet with LasI: negative control growth; results thrown out		pTet with LasI: negative control growth; results thrown out
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	RFP: 20 colonies, 1 picked		RFP: 20 colonies, 1 picked
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock		Glycerol Stock
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page56" class='notebook_page'>		<div id="page56" class='notebook_page'>
Line 3,784:		Line 3,784:
	LuxR-DT-pLux-TetR		LuxR-DT-pLux-TetR
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Minipreps		Minipreps
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxR-DT-pLux-TetR		LuxR-DT-pLux-TetR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas		pLas
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI		LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet		pTet
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac		pLac
	</p>		</p>
-	<br/>	+
	<p>		<p>
	GFP		GFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasI		LasI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page57" class='notebook_page'>		<div id="page57" class='notebook_page'>
Line 3,829:		Line 3,829:
	August 19		August 19
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stocks		Glycerol Stocks
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,863:		Line 3,863:
	Oscillator Population Tests (population with induction plasmid)		Oscillator Population Tests (population with induction plasmid)
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,890:		Line 3,890:
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,920:		Line 3,920:
	Digestion and gel extraction		Digestion and gel extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 3,956:		Line 3,956:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,004:		Line 4,004:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 19, 2012		August 19, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock/Plasmid Isolation		Glycerol Stock/Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP		RFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Toggle Switch in Chlorabackbone		Toggle Switch in Chlorabackbone
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Inoculation		Inoculation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA		RFP-LVA
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion/Extraction		Digestion/Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac: S and P		pLac: S and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxR-DT-pLux-TetR: X and P		LuxR-DT-pLux-TetR: X and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet: S and P		pTet: S and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasI: X and P		LasI: X and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasR-DT: X and P		LasR-DT: X and P
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page61" class='notebook_page'>		<div id="page61" class='notebook_page'>
Line 4,065:		Line 4,065:
	LacI: S and P		LacI: S and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP: X and P		RFP: X and P
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 20		August 20
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,109:		Line 4,109:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,134:		Line 4,134:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 20, 2012		August 20, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligations/Transformations		Ligations/Transformations
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LuxR-DT-pLux-TetR into pLac		LuxR-DT-pLux-TetR into pLac
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasI into pTet		LasI into pTet
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LasR-DT into pTet		LasR-DT into pTet
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP into LacI		RFP into LacI
	</p>		</p>
-	<br/>	+
-	<br/>	+
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 21		August 21
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock and plasmid isolation		Glycerol Stock and plasmid isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,206:		Line 4,206:
	Digestion and gel extraction		Digestion and gel extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,236:		Line 4,236:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,245:		Line 4,245:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,263:		Line 4,263:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 21, 2012		August 21, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colony Pickings		Colony Pickings
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LuxR-DT-pLux-TetR: 24 colonies, 1 picked		pLac-LuxR-DT-pLux-TetR: 24 colonies, 1 picked
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasI: 30 colonies, 3 picked		pTet-LasI: 30 colonies, 3 picked
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasR-DT: 100 colonies, 2 picked		pTet-LasR-DT: 100 colonies, 2 picked
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI-RFP: 24 colonies, 2 picked		LacI-RFP: 24 colonies, 2 picked
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 22		August 22
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Digestion (test)		Digestion (test)
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,339:		Line 4,339:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,348:		Line 4,348:
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,363:		Line 4,363:
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,372:		Line 4,372:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,385:		Line 4,385:
	Population Testing Prep (pop2, pop3, pop2+pop3)		Population Testing Prep (pop2, pop3, pop2+pop3)
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,408:		Line 4,408:
	</ul>		</ul>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 22, 2012		August 22, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock/Miniprep		Glycerol Stock/Miniprep
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LuxR-DT-pLux-TetR		pLac-LuxR-DT-pLux-TetR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasI		pTet-LasI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasR-DT		pTet-LasR-DT
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI-RFP		LacI-RFP
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion/Extraction:		Digestion/Extraction:
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LuxR-DT-pLux-TetR: S and P		pLac-LuxR-DT-pLux-TetR: S and P
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page67" class='notebook_page'>		<div id="page67" class='notebook_page'>
Line 4,452:		Line 4,452:
	GFP: X and P		GFP: X and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI-RFP: S and P		LacI-RFP: S and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LuxI: X and P		pLac-LuxI: X and P
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Test Digestions		Test Digestions
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pTet-LasI: E and P		pTet-LasI: E and P
Line 4,471:		Line 4,471:
	pTet-LasR-DT: E and P		pTet-LasR-DT: E and P
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Ligations/Transformations		Ligations/Transformations
	</p>		</p>
-	<br/>	+
	<p>		<p>
	GFP into pLac-LuxR-DT-pLux-TetR		GFP into pLac-LuxR-DT-pLux-TetR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LuxI into LacI-RFP		pLac-LuxI into LacI-RFP
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Inoculations		Inoculations
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA		RFP-LVA
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-GFP		pTet-GFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RBS-YFP		RBS-YFP
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	August 22		August 22
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Digestion (test)		Digestion (test)
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,545:		Line 4,545:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,554:		Line 4,554:
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,569:		Line 4,569:
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,578:		Line 4,578:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page69" class='notebook_page'>		<div id="page69" class='notebook_page'>
Line 4,590:		Line 4,590:
	Population Testing Prep (pop2, pop3, pop2+pop3)		Population Testing Prep (pop2, pop3, pop2+pop3)
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,613:		Line 4,613:
	</ul>		</ul>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 23, 2012		August 23, 2012
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Colony Picking		Colony Picking
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI-RFP-ST-pLac-LuxI; 40 colonies, 1 picked		LacI-RFP-ST-pLac-LuxI; 40 colonies, 1 picked
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI-RFP-ST-pLac-LuxI; 30 colonies, 1 picked		LacI-RFP-ST-pLac-LuxI; 30 colonies, 1 picked
	</p>		</p>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 23		August 23
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock		Glycerol Stock
	</p>		</p>
-	<br/>	+
	</div>		</div>
Line 4,667:		Line 4,667:
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,676:		Line 4,676:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,691:		Line 4,691:
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,700:		Line 4,700:
	Population Testing from 8/22 prep after 24 hours		Population Testing from 8/22 prep after 24 hours
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,706:		Line 4,706:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<ul>		<ul>
Line 4,765:		Line 4,765:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,803:		Line 4,803:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,833:		Line 4,833:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,842:		Line 4,842:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 24, 2012		August 24, 2012
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI-RFP-ST-pLac-LuxI		LacI-RFP-ST-pLac-LuxI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion/Extraction		Digestion/Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasR-DT		pTet-LasR-DT
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasI		pTet-LasI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI-RFP-ST-pLac-LuxI		LacI-RFP-ST-pLac-LuxI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas		pLas
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasI into pLac-LuxR-DT-pLux-TetR-GFP-ST		pTet-LasI into pLac-LuxR-DT-pLux-TetR-GFP-ST
	</p>		</p>
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page74" class='notebook_page'>		<div id="page74" class='notebook_page'>
Line 4,897:		Line 4,897:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	</div>		</div>
	August 24		August 24
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,914:		Line 4,914:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,926:		Line 4,926:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 4,938:		Line 4,938:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 25, 2012		August 25, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock/Plasmid Isolation		Glycerol Stock/Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI-RFP-ST-pLac-LuxI		LacI-RFP-ST-pLac-LuxI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion/Extraction		Digestion/Extraction
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page75" class='notebook_page'>		<div id="page75" class='notebook_page'>
Line 4,968:		Line 4,968:
	LacI-RFP-ST-pLac-LuxI		LacI-RFP-ST-pLac-LuxI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	LacI-RFP-ST-pLac-LuxI into pLas		LacI-RFP-ST-pLac-LuxI into pLas
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 25		August 25
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock		Glycerol Stock
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,008:		Line 5,008:
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,026:		Line 5,026:
	Digestion and Gel Extraction		Digestion and Gel Extraction
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page76" class='notebook_page'>		<div id="page76" class='notebook_page'>
Line 5,048:		Line 5,048:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,072:		Line 5,072:
	Primer Dilution		Primer Dilution
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,087:		Line 5,087:
	Colony PCR		Colony PCR
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page77" class='notebook_page'>		<div id="page77" class='notebook_page'>
Line 5,110:		Line 5,110:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,141:		Line 5,141:
	August 26, 2012		August 26, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colony Picking		Colony Picking
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas-LacI-RFP-ST-pLac-LuxI; 50 colonies, 1 picked		pLas-LacI-RFP-ST-pLac-LuxI; 50 colonies, 1 picked
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Inoculation		Inoculation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP		pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas-LacI-RFP-ST-pLac-LuxI		pLas-LacI-RFP-ST-pLac-LuxI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 26		August 26
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies Picked		Colonies Picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,184:		Line 5,184:
	Population testing		Population testing
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,204:		Line 5,204:
	</ul>		</ul>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 27, 2012		August 27, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock/Plasmid Isolation		Glycerol Stock/Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP		pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas-LacI-RFP-ST-pLac-LuxI		pLas-LacI-RFP-ST-pLac-LuxI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	PCR		PCR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Add second LacO site to pLac		Add second LacO site to pLac
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion/Extraction		Digestion/Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP		pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas-LacI-RFP-ST-pLac-LuxI		pLas-LacI-RFP-ST-pLac-LuxI
	</p>		</p>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 27		August 27
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,269:		Line 5,269:
	Digestion and Gel extraction		Digestion and Gel extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,281:		Line 5,281:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,308:		Line 5,308:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,341:		Line 5,341:
	</ul>		</ul>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 28, 2012		August 28, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Primer Dilutions		Primer Dilutions
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 28		August 28
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colony PCR		Colony PCR
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,375:		Line 5,375:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,387:		Line 5,387:
	Population Testing		Population Testing
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,393:		Line 5,393:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,419:		Line 5,419:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,442:		Line 5,442:
	Digestion and Gel Extraction		Digestion and Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,451:		Line 5,451:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,471:		Line 5,471:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 29, 2012		August 29, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLas-LacI-RFP-ST-pLac-LuxI into pTet-LasR-DT		pLas-LacI-RFP-ST-pLac-LuxI into pTet-LasR-DT
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 29		August 29
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Population Test Prep		Population Test Prep
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,529:		Line 5,529:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 30		August 30
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,560:		Line 5,560:
	Colony PCR		Colony PCR
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,566:		Line 5,566:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,578:		Line 5,578:
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,584:		Line 5,584:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page85" class='notebook_page'>		<div id="page85" class='notebook_page'>
Line 5,590:		Line 5,590:
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 31, 2012		August 31, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colony picking		Colony picking
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI; 50 colonies, 1 picked		pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI; 50 colonies, 1 picked
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	PCR Reaction		PCR Reaction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Add LacO binding site to pLac; 65.2 degrees found to be optimal temperature for elongation		Add LacO binding site to pLac; 65.2 degrees found to be optimal temperature for elongation
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO		pLac-LacO
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	August 31		August 31
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Glycerol Stock and Plasmid Isolation		Glycerol Stock and Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,645:		Line 5,645:
	Digestion and Gel Extraction		Digestion and Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,659:		Line 5,659:
	Colony PCR		Colony PCR
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,665:		Line 5,665:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,677:		Line 5,677:
	Population Test Results		Population Test Results
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,702:		Line 5,702:
	</ul>		</ul>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 1, 2012		September 1, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock/Plasmid Isolation		Glycerol Stock/Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI		pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI
Line 5,720:		Line 5,720:
	</div>		</div>
	<div id="page87" class='notebook_page'>		<div id="page87" class='notebook_page'>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Colony Picking		Colony Picking
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO		pLac-LacO
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Inoculations		Inoculations
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-TetR		pLac-TetR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-TetR-RBS-RFP		pLac-TetR-RBS-RFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI		pTet-LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-RBS-GFP-T		pTet-RBS-GFP-T
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 1		September 1
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligations		Ligations
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,798:		Line 5,798:
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,810:		Line 5,810:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,816:		Line 5,816:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page89" class='notebook_page'>		<div id="page89" class='notebook_page'>
Line 5,823:		Line 5,823:
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 2, 2012		September 2, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock/Plasmid Isolation		Glycerol Stock/Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO		pLac-LacO
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 3, 2012		September 3, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-TetR		pLac-TetR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-TetR-RBS-RFP		pLac-TetR-RBS-RFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI		pTet-LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-RBS-GFP-T		pTet-RBS-GFP-T
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion/Extraction		Digestion/Extraction
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pLac-TetR		pLac-TetR
Line 5,876:		Line 5,876:
	pLac-TetR-RBS-RFP		pLac-TetR-RBS-RFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI		pTet-LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-RBS-GFP-T		pTet-RBS-GFP-T
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page90" class='notebook_page'>		<div id="page90" class='notebook_page'>
Line 5,890:		Line 5,890:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 3		September 3
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock		Glycerol Stock
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,910:		Line 5,910:
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,925:		Line 5,925:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,940:		Line 5,940:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,964:		Line 5,964:
	Colony PCR for I3-98-2 and I4-1-2-		Colony PCR for I3-98-2 and I4-1-2-
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 5,977:		Line 5,977:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 4, 2012		September 4, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Inoculations		Inoculations
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI (Atc 10 microliters)		pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI (Atc 10 microliters)
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI (IPTG 10 microliters)		pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI (IPTG 10 microliters)
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 4		September 4
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,021:		Line 6,021:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,030:		Line 6,030:
	Colonies picked from I4-1-2		Colonies picked from I4-1-2
	</p>		</p>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 5, 2012		September 5, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Inoculations		Inoculations
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI		pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI
Line 6,051:		Line 6,051:
	pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI		pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	1 with Atc		1 with Atc
	</p>		</p>
-	<br/>	+
	<p>		<p>
	1 with IPTG		1 with IPTG
	</p>		</p>
-	<br/>	+
	<p>		<p>
	1 with No induction		1 with No induction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 5		September 5
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock and Plasmid Isolation		Glycerol Stock and Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,101:		Line 6,101:
	Digestion and Gel Extraction		Digestion and Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,125:		Line 6,125:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,134:		Line 6,134:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page94" class='notebook_page'>		<div id="page94" class='notebook_page'>
Line 6,140:		Line 6,140:
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 6, 2012		September 6, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Sequencing		Sequencing
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI		pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI		pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO		pLac-LacO
	</p>		</p>
-	<br/>	+
	<p>		<p>
	RFP-LVA		RFP-LVA
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Inoculations		Inoculations
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Chlorabackbone		Chlorabackbone
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI		pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI
Line 6,181:		Line 6,181:
	pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI		pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	1 with Atc		1 with Atc
	</p>		</p>
-	<br/>	+
	<p>		<p>
	1 with IPTG		1 with IPTG
	</p>		</p>
-	<br/>	+
	<p>		<p>
	1 with No induction		1 with No induction
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion/Extraction		Digestion/Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Chlorabackbone		Chlorabackbone
	</p>		</p>
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page95" class='notebook_page'>		<div id="page95" class='notebook_page'>
Line 6,209:		Line 6,209:
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 6		September 6
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation and Glycerol Stock		Plasmid Isolation and Glycerol Stock
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,232:		Line 6,232:
	Digestion and Gel Extraction		Digestion and Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,247:		Line 6,247:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,276:		Line 6,276:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 7, 2012		September 7, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Inoculation		Inoculation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP		pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-RBS-GFP-T		pLac-RBS-GFP-T
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pTet-RBS-GFP-T-pLac-TetR		pTet-RBS-GFP-T-pLac-TetR
Line 6,359:		Line 6,359:
	RBS-GFP-ST		RBS-GFP-ST
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 7		September 7
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Test Digestions		Test Digestions
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,388:		Line 6,388:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,394:		Line 6,394:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 8, 2012		September 8, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Time-Lapse Experiment to observe toggle switch		Time-Lapse Experiment to observe toggle switch
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP		pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP
Line 6,417:		Line 6,417:
	pLac-RBS-GFP-T		pLac-RBS-GFP-T
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pTet-RBS-GFP-T-pLac-TetR		pTet-RBS-GFP-T-pLac-TetR
Line 6,477:		Line 6,477:
	GFP		GFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligations		Ligations
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-TetR into Chlora		pLac-TetR into Chlora
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-RBS-GFP-T into Chlora		pLac-RBS-GFP-T into Chlora
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-RBS-GFP-T into Chlora		pTet-RBS-GFP-T into Chlora
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI into Chlora		pTet-LacI into Chlora
	</p>		</p>
-	<br/>	+
-	<br/>]	+	]
	</div>		</div>
	<div id="page100" class='notebook_page'>		<div id="page100" class='notebook_page'>
Line 6,504:		Line 6,504:
	Digestion/Extraction		Digestion/Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	GFP		GFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO		pLac-LacO
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP		pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP
Line 6,519:		Line 6,519:
	pLac-RBS-GFP-T		pLac-RBS-GFP-T
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pTet-RBS-GFP-T-pLac-TetR		pTet-RBS-GFP-T-pLac-TetR
Line 6,567:		Line 6,567:
	pLac-LuxI		pLac-LuxI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 8		September 8
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies Picked		Colonies Picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,591:		Line 6,591:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page102" class='notebook_page'>		<div id="page102" class='notebook_page'>
Line 6,598:		Line 6,598:
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 9, 2012		September 9, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colony Picking		Colony Picking
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-TetR, 1 colony picked		pLac-TetR, 1 colony picked
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-RBS-GFP-T, 1 colony picked		pLac-RBS-GFP-T, 1 colony picked
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI, 1 colony picked		pTet-LacI, 1 colony picked
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO-GFP		pLac-LacO-GFP
	</p>		</p>
-	<br/>	+
-	<br/>	+
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 9		September 9
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock and Plasmid Isolation		Glycerol Stock and Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,654:		Line 6,654:
	Digestion and Gel Extraction		Digestion and Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,671:		Line 6,671:
	Ligations		Ligations
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,698:		Line 6,698:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 10, 2012		September 10, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock/Miniprep		Glycerol Stock/Miniprep
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-TetR		pLac-TetR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-RBS-GFP-T		pLac-RBS-GFP-T
Line 6,724:		Line 6,724:
	pTet-LacI		pTet-LacI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Gel Extraction		Gel Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO-GFP		pLac-LacO-GFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI		pTet-LacI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI into pLac-LacO-RBS-GFP-ST		pTet-LacI into pLac-LacO-RBS-GFP-ST
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-RBS-GFP-T-pTet-LacI into chlora backbone		pLac-RBS-GFP-T-pTet-LacI into chlora backbone
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate Projects		Triple Population and AND Gate Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 10		September 10
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Colonies Picked		Colonies Picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,795:		Line 6,795:
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,804:		Line 6,804:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 11, 2012		September 11, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	PCR		PCR
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO		pLac-LacO
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO x2		pLac-LacO x2
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 12, 2012		September 12, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Digestion/Gel Extraction		Digestion/Gel Extraction
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI-pLac-LacO-RBS-GFP-ST		pTet-LacI-pLac-LacO-RBS-GFP-ST
	</p>		</p>
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page106" class='notebook_page'>		<div id="page106" class='notebook_page'>
Line 6,848:		Line 6,848:
	Experiments		Experiments
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Toggle with 20 micro liters IPTG		Toggle with 20 micro liters IPTG
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Toggle with 40 micro liters ATc		Toggle with 40 micro liters ATc
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Strain 1 of dual population toggle with inducer strain		Strain 1 of dual population toggle with inducer strain
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Strain 2 of dual population toggle with inducer strain		Strain 2 of dual population toggle with inducer strain
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO-RBS-GFP-ST-pTet-LacI into chlora backbone		pLac-LacO-RBS-GFP-ST-pTet-LacI into chlora backbone
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate		Triple Population and AND Gate
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 13		September 13
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock and Plasmid Isolation		Glycerol Stock and Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,925:		Line 6,925:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,949:		Line 6,949:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 6,987:		Line 6,987:
	Ligations for Chlora		Ligations for Chlora
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,002:		Line 7,002:
	Transformation		Transformation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,020:		Line 7,020:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,037:		Line 7,037:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 14, 2012		September 14, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP into Chlora backbone		pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP into Chlora backbone
Line 7,056:		Line 7,056:
	pLac-RBS-GFP-T into Chlora backbone		pLac-RBS-GFP-T into Chlora backbone
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pTet-RBS-GFP-T-pLac-TetR into Chlora backbone		pTet-RBS-GFP-T-pLac-TetR into Chlora backbone
Line 7,108:		Line 7,108:
	pLac-LacO x2-RBS-GFP-ST-pTet-LacI		pLac-LacO x2-RBS-GFP-ST-pTet-LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock/Plasmid Isolation		Glycerol Stock/Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO-GFP		pLac-LacO-GFP
	</p>		</p>
-	<br/>	+
-	<br/>	+
	<p>		<p>
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-LacO-GFP		pLac-LacO-GFP
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pTet-LacI		pTet-LacI
	</p>		</p>
-	<br/>	+
	<p dir="ltr">		<p dir="ltr">
	pLac-LacO x2-RBS-GFP-ST-pTet-LacI		pLac-LacO x2-RBS-GFP-ST-pTet-LacI
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Inoculation		Inoculation
	</p>		</p>
-	<br/>	+
	<p>		<p>
	pLac-GFP-pTet-LacI		pLac-GFP-pTet-LacI
	</p>		</p>
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page111" class='notebook_page'>		<div id="page111" class='notebook_page'>
Line 7,148:		Line 7,148:
	Triple Population and AND Gate		Triple Population and AND Gate
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 15		September 15
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,180:		Line 7,180:
	Colonies picked		Colonies picked
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,198:		Line 7,198:
	Digestion		Digestion
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,224:		Line 7,224:
	Ligations		Ligations
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,248:		Line 7,248:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,271:		Line 7,271:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page114" class='notebook_page'>		<div id="page114" class='notebook_page'>
Line 7,279:		Line 7,279:
	Triple Population and AND Gate		Triple Population and AND Gate
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 18		September 18
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock and Plasmid Isolation		Glycerol Stock and Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,302:		Line 7,302:
	Digestion and Gel Extraction		Digestion and Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,320:		Line 7,320:
	Ligation		Ligation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,355:		Line 7,355:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,370:		Line 7,370:
	Testing AND Gate		Testing AND Gate
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,382:		Line 7,382:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Triple Population and AND Gate		Triple Population and AND Gate
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 19		September 19
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plate Experiments for AND Gate		Plate Experiments for AND Gate
	</p>		</p>
-	<br/>	+
-	<br/>	+
-	<br/>	+
-	<br/>	+
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page116" class='notebook_page'>		<div id="page116" class='notebook_page'>
Line 7,405:		Line 7,405:
	Triple Population and AND Gate		Triple Population and AND Gate
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 20		September 20
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Glycerol Stock and Plasmid Isolation		Glycerol Stock and Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,434:		Line 7,434:
	Digestions (repeated 3X)		Digestions (repeated 3X)
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,452:		Line 7,452:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page117" class='notebook_page'>		<div id="page117" class='notebook_page'>
Line 7,458:		Line 7,458:
	Triple Population and AND Gate		Triple Population and AND Gate
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 22		September 22
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligations (Repeated)		Ligations (Repeated)
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,496:		Line 7,496:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	<p>		<p>
	Single and Double Population Projects		Single and Double Population Projects
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 22, 2012		September 22, 2012
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Single toggle experiment - No induction at start, check every 6 hours, induce with IPTG after first 6 hours, and induce with ATc in new tube after 12 Hours		Single toggle experiment - No induction at start, check every 6 hours, induce with IPTG after first 6 hours, and induce with ATc in new tube after 12 Hours
	</p>		</p>
-	<br/>	+
	</div>		</div>
	<div id="page118" class='notebook_page'>		<div id="page118" class='notebook_page'>
Line 7,514:		Line 7,514:
	Triple Population and AND Gate		Triple Population and AND Gate
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 22		September 22
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Ligations (Repeated)		Ligations (Repeated)
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,552:		Line 7,552:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
	</div>		</div>
	<div id="page119" class='notebook_page'>		<div id="page119" class='notebook_page'>
Line 7,558:		Line 7,558:
	Triple Population and AND Gate		Triple Population and AND Gate
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 23		September 23
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Digestions		Digestions
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,587:		Line 7,587:
	Innoculations for AND Gate		Innoculations for AND Gate
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,602:		Line 7,602:
	Testing AND Gate		Testing AND Gate
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,619:		Line 7,619:
	Innoculation		Innoculation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,640:		Line 7,640:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+
-	<br/>	+
	</div>		</div>
	<div id="page121" class='notebook_page'>		<div id="page121" class='notebook_page'>
Line 7,647:		Line 7,646:
	Triple Population and AND Gate		Triple Population and AND Gate
	</p>		</p>
-	<br/>	+
	<p>		<p>
	September 23		September 23
	</p>		</p>
-	<br/>	+
	<p>		<p>
	Plasmid Isolation		Plasmid Isolation
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,673:		Line 7,672:
	Digestions and Gel Extraction		Digestions and Gel Extraction
	</p>		</p>
-	<br/>	+
	<ul>		<ul>
	<li>		<li>
Line 7,688:		Line 7,687:
	</li>		</li>
	</ul>		</ul>
-	<br/>	+	</div>
	</html>		</html>

---

Latest revision as of 01:28, 4 October 2012

August 24

Plasmid Isolation

• pluxR-LasI-RBS-RFP: Concentration: 42.6

Digestion

• pLuxR-LasI-RBS-RFP (X/P)
• pLuxR-araC-lasR-TT (X/P)

Innoculation

• pLuxR-LasI-RBS-RFP
• pLuxR-araC-LasR-TT
• pLsrA-LuxI-YFP

Single and Double Population Projects

August 25, 2012

Glycerol Stock/Plasmid Isolation

LacI-RFP-ST-pLac-LuxI

Digestion/Extraction