Team:Caltech

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<h1>From Biofilms to Biofuel</h1>
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<h1>Biofuels and Bacterial Animations</h1>
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Caltech’s 2012 iGEM <a href="https://2012.igem.org/Team:Caltech/Team">team</a> aims to manipulate bacteria to <a href="https://2012.igem.org/Team:Caltech/Project#Degradation_Project">degrade</a> stable organic polymers such as lignin and alginate; to use these substrates to <a href="https://2012.igem.org/Team:Caltech/Project#Biofuel_Project">synthesize biofuels</a>, specifically ethanol; and to direct their ATP synthesis mechanism to rely on <a href="https://2012.igem.org/Team:Caltech/Project#Proteorhodopsin_Project">proteorhodopsin</a> as the source of the proton gradient, freeing NADH to interact in the synthetic pathway instead.  We will develop the three facets of the project in different strains of E. coli.  We will then splice the manipulated sequences into one new organism.
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The Caltech 2012 iGEM <a href="https://2012.igem.org/Team:Caltech/Team">team's</a> project focused on manipulating bacteria to <a href="https://2012.igem.org/Team:Caltech/Project#Degradation_Project">degrade</a> stable organic polymers such as lignin and alginate; using these substrates to synthesize biofuels, specifically ethanol; and directing their ATP synthesis mechanism to rely on <a href="https://2012.igem.org/Team:Caltech/Project#Proteorhodopsin_Project">proteorhodopsin</a> as the source of the proton gradient, freeing NADH to interact in the synthetic pathway.  Caltech also collaborated with California Institute of the Arts to create an animation using a plate of E. coli transformed with mCherry plasmid.  To learn more about our collaboration, view our <a href="https://2012.igem.org/Team:Caltech/Human_Practice">Human Practice</a> page.
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<img src="https://static.igem.org/mediawiki/2012/c/ca/Caltech_Flowchart.png" alt="Project Flowchart"/>
<img src="https://static.igem.org/mediawiki/2012/c/ca/Caltech_Flowchart.png" alt="Project Flowchart"/>
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Caltech is also collaborating with California Institute of the Arts to create an animation using a plate of E. coli transformed with mcherry plasmid.
 
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Latest revision as of 00:42, 4 October 2012



Biofuels and Bacterial Animations

The Caltech 2012 iGEM team's project focused on manipulating bacteria to degrade stable organic polymers such as lignin and alginate; using these substrates to synthesize biofuels, specifically ethanol; and directing their ATP synthesis mechanism to rely on proteorhodopsin as the source of the proton gradient, freeing NADH to interact in the synthetic pathway. Caltech also collaborated with California Institute of the Arts to create an animation using a plate of E. coli transformed with mCherry plasmid. To learn more about our collaboration, view our Human Practice page.

Project Flowchart

Retrieved from "http://2012.igem.org/Team:Caltech"