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Team:UCSF/Medal
From 2012.igem.org
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<regulartext> YoeB and YefM are new submissions to the registry and we have demonstrated that both constructs and parts work as desired. <br> Additionally, VioABE is an improvement on the previous submitted part by the 2009 Cambridge iGEM team (<a href=http://partsregistry.org/wiki/index.php/Part:BBa_K274003>BBa_K274003</a>). This is because our part contains only the three necessary genes to produce the green pigment. It is smaller and easier to clone, yet achieves the desired output. | <regulartext> YoeB and YefM are new submissions to the registry and we have demonstrated that both constructs and parts work as desired. <br> Additionally, VioABE is an improvement on the previous submitted part by the 2009 Cambridge iGEM team (<a href=http://partsregistry.org/wiki/index.php/Part:BBa_K274003>BBa_K274003</a>). This is because our part contains only the three necessary genes to produce the green pigment. It is smaller and easier to clone, yet achieves the desired output. | ||
| + | <br> As reported by other teams in the Experience section, attempts to retrieve the Cambridge submitted part from the registry have failed. The restriction sites were not correct and we found that it failed as a PCR template for any of the four genes reported. Therefore, we obtained a template from another source (Dueber Lab, UC Berkeley) and are submitting the DNA for these three enzymes for use by future iGEM teams. | ||
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<div id="rightcontent"> | <div id="rightcontent"> | ||
Revision as of 19:37, 3 October 2012
- Part: BBa_K726012
- Link: BBa_K726012
- Part: BBa_K726014
- Link: BBa_K726014
- Part: BBa_K726016
- Link: BBa_K726016
Additionally, VioABE is an improvement on the previous submitted part by the 2009 Cambridge iGEM team (BBa_K274003). This is because our part contains only the three necessary genes to produce the green pigment. It is smaller and easier to clone, yet achieves the desired output.
As reported by other teams in the Experience section, attempts to retrieve the Cambridge submitted part from the registry have failed. The restriction sites were not correct and we found that it failed as a PCR template for any of the four genes reported. Therefore, we obtained a template from another source (Dueber Lab, UC Berkeley) and are submitting the DNA for these three enzymes for use by future iGEM teams.
