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Team:Michigan/Notebook
From 2012.igem.org
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<p> <span style="background-color:#E0B91B;font-family:Courier, monospace;">XbaI</span></p> | <p> <span style="background-color:#E0B91B;font-family:Courier, monospace;">XbaI</span></p> | ||
<p> <span style="background-color:#C8E0DB;font-family:Courier, monospace;">SpeI</span></p> | <p> <span style="background-color:#C8E0DB;font-family:Courier, monospace;">SpeI</span></p> | ||
| - | <p> <span style="background-color:# | + | <p> <span style="background-color:#A9C9A4;font-family:Courier, monospace;">PstI</span></p> |
| - | <p> <span style="background-color:# | + | <p> <span style="background-color:#86C67C;font-family:Courier, monospace;">AgeI</span></p> |
| - | <p> <span style="background-color:# | + | <p> <span style="background-color:#CD96CD;font-family:Courier, monospace;">NgoMIV</span></p> |
| - | <p><br/>HbiF possesses an N-terminus in the (truncated) RFC10 format : | + | <p><br/>HbiF possesses an N-terminus in the (truncated) RFC10 format :</p> |
<p style="font-family:Courier, monospace;"><span style="background-color:#E0771B">GAATTC</span> GCGGCCGC T <span style="background-color:#E0B91B;">TCTAG</span> [ATG]</p> | <p style="font-family:Courier, monospace;"><span style="background-color:#E0771B">GAATTC</span> GCGGCCGC T <span style="background-color:#E0B91B;">TCTAG</span> [ATG]</p> | ||
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| + | <p><br/>No extra amino acids are created by this prefix, in comparison to the standard RFC25 prefix, which creates a serine and glycine. The suffix proceeds as follows:</p> | ||
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| + | <p style="font-family:Courier, monospace;"><span style="background-color:#86C67C;">ACCGGT</span> TAAT <span style="background-color:#C8E0DB">ACTAGT</span> A GCGGCCG <span style="background-color:#A9C9A4">CTGCAG</span> </p> | ||
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<h2>Prepare FimE for protein expression</h2> | <h2>Prepare FimE for protein expression</h2> | ||
Revision as of 00:07, 6 July 2012
Team:Michigan/Notebook/Protocols
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Modify HbiF into a Biobrick compatible format
HbiF and LVA degradation tag were ordered from IDT with the following specifications.
EcoRI
XbaI
SpeI
PstI
AgeI
NgoMIV
HbiF possesses an N-terminus in the (truncated) RFC10 format :
GAATTC GCGGCCGC T TCTAG [ATG]
No extra amino acids are created by this prefix, in comparison to the standard RFC25 prefix, which creates a serine and glycine. The suffix proceeds as follows:
ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG
Prepare FimE for protein expression
Team Reports
