Team:Berkeley/Project/Zippers

From 2012.igem.org

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What the point of our leucine zipper experiment was.
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Protein interaction domains (coiled coils, PDZ, SH3, GBD, etc) have been used by synthetic biologists to oligomerize or localize proteins. In particular, they have been applied to both scaffolding and signaling applications with impressive results. However, the limited number and diversity of well-characterized, orthogonal protein interaction domains restricts the complexity of systems that can be designed.
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Revision as of 00:49, 3 October 2012

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iGEM Berkeley iGEMBerkeley iGEMBerkeley

Mercury

Protein interaction domains (coiled coils, PDZ, SH3, GBD, etc) have been used by synthetic biologists to oligomerize or localize proteins. In particular, they have been applied to both scaffolding and signaling applications with impressive results. However, the limited number and diversity of well-characterized, orthogonal protein interaction domains restricts the complexity of systems that can be designed.


Why we bound mKate to bait and PTS1 to prey. Includes images showing preliminary results.


Our home run experiment with 40 bait and 40 prey.